Specific Detection and Identification of Herpes B Virus by a PCR-Microplate Hybridization Assay
ABSTRACT
Herpes B virus DNA was specifically amplified by PCR, targeting the regions that did not cross-react with herpes simplex virus (HSV). The amplified products, which were shown to be highly genetic polymorphisms among herpes B virus isolates, were identified by microplate hybridization with probes generated by PCR. The products immobilized in microplate wells were hybridized with the biotin-labeled probes derived from the SMHV strain of herpes B virus. The amplified products derived from the SMHV and E2490 strains of herpes B virus were identified by microplate hybridization. PCR products amplified from the trigeminal ganglia of seropositive cynomolgus macaques were identified as herpes B virus DNA. The utility of the PCR-microplate hybridization assay for genetic detection and identification of the polymorphic region of herpes B virus was determined.
FOOTNOTES
- Received 2 September 2003.
- Returned for modification 9 November 2003.
- Accepted 1 February 2004.
- ↵*Corresponding author. Mailing address: Department of Veterinary Public Health, Nippon Veterinary and Animal Science University, 1-7-1 Kyonan, Musashino, Tokyo 180-8602, Japan. Phone: 81-422-31-4151, ext. 282. Fax: 81-422-30-7531. E-mail: nvau-vph{at}interlink.or.jp.
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↵† Present address: Department of Molecular Immunology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan.
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↵‡ Present address: Laboratory Animal Research Center, The Institute of Medical Science, The University of Tokyo, Minato-ku, Tokyo 108-8639, Japan.
- American Society for Microbiology
