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Journal of Clinical Microbiology
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Advanced Search

  • Home
  • Articles
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    • Submit a Manuscript
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  • Specimen Collection from Both Ends of At-Home Midturbinate Swabs
    Specimen Collection from Both Ends of At-Home Midturbinate Swabs
  • Salmonella enterica Serovar Typhi in a Central American Refugee
    Salmonella enterica Serovar Typhi in a Central American Refugee
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  • Current Issue
  • Accepted Manuscripts
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Photo Quiz

  • Photo Quiz: Strength in Numbers—a Disseminated Infection Causing Shortness of Breath
    Photo Quiz
    Photo Quiz: Strength in Numbers—a Disseminated Infection Causing Shortness of Breath
    Chelsea E. Modlin, Jessica Howard-Anderson, Ashley M. Greer, Christine E. Marchioli, Edmund K. Waller, Aneesh K. Mehta, Eileen M. Burd, Colleen S. Kraft, Ahmed Babiker
  • Answer to May 2021 Photo Quiz
    Photo Quiz
    Answer to May 2021 Photo Quiz
    Chelsea E. Modlin, Jessica Howard-Anderson, Ashley M. Greer, Christine E. Marchioli, Edmund K. Waller, Aneesh K. Mehta, Eileen M. Burd, Colleen S. Kraft, Ahmed Babiker

Editorials

  • Free
    2021 American Society for Microbiology Awards Program: Clinical Microbiology Honorees
    Editorial
    2021 American Society for Microbiology Awards Program: Clinical Microbiology Honorees
    Erik Munson
  • Free
    Fun Classifying Fungal Infections: a Micro-Comic Strip
    Editorial
    Fun Classifying Fungal Infections: a Micro-Comic Strip
    Alexander J. McAdam

The Brief Case

  • Free
    The Brief Case: <span class="named-content genus-species" id="named-content-1">Salmonella enterica</span> Serovar Typhi in a Central American Refugee
    The Brief Case
    The Brief Case: Salmonella enterica Serovar Typhi in a Central American Refugee
    Joel Addams, William Lainhart
  • Free
    Closing the Brief Case: <span class="named-content genus-species" id="named-content-1">Salmonella enterica</span> Serovar Typhi in a Central American Refugee
    The Brief Case
    Closing the Brief Case: Salmonella enterica Serovar Typhi in a Central American Refugee
    Joel Addams, William Lainhart

Commentary

  • Free
    Approach to Assessment of New Swabs and Viral Transport Media for SARS-CoV-2 Testing
    Commentary
    Approach to Assessment of New Swabs and Viral Transport Media for SARS-CoV-2 Testing

    In light of the present pandemic of novel coronavirus disease 2019 (COVID-19) and the unprecedented high demand for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) testing worldwide, there are shortages of established specimen collection devices for respiratory viral testing for diagnostic microbiology laboratories. This creates the need to validate unverified collection devices from manufacturers that may not be a...

    Kerstin Locher, Billie Velapatino, Mélissa Caza, Lisa Li, Charlene Porter, Marthe Charles

Minireviews

  • Unstimulated Pleural Fluid Interferon Gamma for Diagnosis of Tuberculous Pleural Effusion: a Systematic Review and Meta-analysis
    Minireview
    Unstimulated Pleural Fluid Interferon Gamma for Diagnosis of Tuberculous Pleural Effusion: a Systematic Review and Meta-analysis

    Unstimulated interferon gamma may be a useful pleural fluid biomarker in the diagnosis of tuberculous pleural effusion (TPE). However, the exact threshold of pleural fluid interferon gamma and its accuracy during routine clinical decision-making is not clear.

    Ashutosh Nath Aggarwal, Ritesh Agarwal, Sahajal Dhooria, Kuruswamy Thurai Prasad, Inderpaul Singh Sehgal, Valliappan Muthu
  • Free
    Immunologic Testing for SARS-CoV-2 Infection from the Antigen Perspective
    Minireview
    Immunologic Testing for SARS-CoV-2 Infection from the Antigen Perspective

    Coronavirus disease 2019 (COVID-19), caused by the novel coronavirus severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has spread globally as a severe pandemic. SARS-CoV-2 infection stimulates antigen-specific antibody responses.

    Dandan Li, Jinming Li
  • Free
    Performance of Saliva, Oropharyngeal Swabs, and Nasal Swabs for SARS-CoV-2 Molecular Detection: a Systematic Review and Meta-analysis
    Minireview
    Performance of Saliva, Oropharyngeal Swabs, and Nasal Swabs for SARS-CoV-2 Molecular Detection: a Systematic Review and Meta-analysis

    Nasopharyngeal (NP) swabs are considered the highest-yield sample for diagnostic testing for respiratory viruses, including SARS-CoV-2. The need to increase capacity for SARS-CoV-2 testing in a variety of settings, combined with shortages of sample collection supplies, have motivated a search for alternative sample types with high sensitivity.

    Rose A. Lee, Joshua C. Herigon, Andrea Benedetti, Nira R. Pollock, Claudia M. Denkinger

Bacteriology

  • Open Access
    Reflex Detection of Ciprofloxacin Resistance in <span class="named-content genus-species" id="named-content-1">Neisseria gonorrhoeae</span> by Use of the SpeeDx ResistancePlus GC Assay
    Bacteriology
    Reflex Detection of Ciprofloxacin Resistance in Neisseria gonorrhoeae by Use of the SpeeDx ResistancePlus GC Assay

    Resistance-guided therapy (RGT) for gonorrhoea may reduce unnecessary use of broad-spectrum antibiotics. When reflexed from the Aptima Combo 2 assay, the ResistancePlus GC assay demonstrated 94.8% sensitivity and 100.0% specificity for Neisseria gonorrhoeae detection.

    Darren Y. J. Lee, Melinda M. Ashcroft, Eric P. F. Chow, Michelle Sait, Vesna De Petra, Marlene Tschaepe, Sigrid Lange, George Taiaroa, Catriona S. Bradshaw, David M. Whiley, Christopher K. Fairley, Benjamin P. Howden, Marcus Y. Chen, Shivani Pasricha, Deborah A. Williamson
  • Evaluation of the Accelerate Pheno System for Rapid Identification and Antimicrobial Susceptibility Testing of Positive Blood Culture Bottles Inoculated with Primary Sterile Specimens from Patients with Suspected Severe Infections
    Bacteriology
    Evaluation of the Accelerate Pheno System for Rapid Identification and Antimicrobial Susceptibility Testing of Positive Blood Culture Bottles Inoculated with Primary Sterile Specimens from Patients with Suspected Severe Infections

    The Accelerate Pheno system is approved for rapid identification and phenotypic antimicrobial susceptibility testing (AST) of microorganisms grown from positive blood cultures inoculated with blood from septic patients. We evaluated the performance of the system for identification and AST from positive blood culture bottles inoculated with primary sterile nonblood specimens from patients with suspected severe infections.

    ...
    V. Chapot, L. Effenberg, J. Dohmen-Ruetten, J. Buer, J. Kehrmann
  • Genomic Surveillance and Improved Molecular Typing of <span class="named-content genus-species" id="named-content-1">Bordetella pertussis</span> Using wgMLST
    Bacteriology
    Genomic Surveillance and Improved Molecular Typing of Bordetella pertussis Using wgMLST

    Multilocus sequence typing (MLST) provides allele-based characterization of bacterial pathogens in a standardized framework. However, classical MLST schemes for Bordetella pertussis, the causative agent of whooping cough, seldom reveal diversity among the small number of gene targets and thereby fail to delineate population structure.

    Michael R. Weigand, Yanhui Peng, Hannes Pouseele, Dane Kania, Katherine E. Bowden, Margaret M. Williams, M. Lucia Tondella
  • Clinical Evaluation of a Real-Time PCR Assay for Simultaneous Detection of <span class="named-content genus-species" id="named-content-1">Helicobacter pylori</span> and Genotypic Markers of Clarithromycin Resistance Directly from Stool
    Bacteriology
    Clinical Evaluation of a Real-Time PCR Assay for Simultaneous Detection of Helicobacter pylori and Genotypic Markers of Clarithromycin Resistance Directly from Stool

    Helicobacter pylori infection is mainly diagnosed noninvasively, with susceptibility testing traditionally requiring endoscopy. Treatment is empirical, with clarithromycin-based triple therapy recommended where resistance rates are below 15%. Rising rates of clarithromycin resistance, resulting in high therapy failure rates, are seen worldwide, but U.S. data are...

    Rebecca Marrero Rolon, Scott A. Cunningham, Jayawant N. Mandrekar, Erin T. Polo, Robin Patel
  • Detection of Tick-Borne Bacteria from Whole Blood Using 16S Ribosomal RNA Gene PCR Followed by Next-Generation Sequencing
    Bacteriology
    Detection of Tick-Borne Bacteria from Whole Blood Using 16S Ribosomal RNA Gene PCR Followed by Next-Generation Sequencing

    Reported cases of tick-borne diseases have steadily increased for more than a decade. In the United States, a majority of tick-borne infections are caused by bacteria.

    Kyle G. Rodino, Matthew J. Wolf, Sarah Sheldon, Luke C. Kingry, Jeannine M. Petersen, Robin Patel, Bobbi S. Pritt
  • <span class="named-content genus-species" id="named-content-1">Helicobacter pylori</span> Antimicrobial Resistance and Gene Variants in High- and Low-Gastric-Cancer-Risk Populations
    Bacteriology
    Helicobacter pylori Antimicrobial Resistance and Gene Variants in High- and Low-Gastric-Cancer-Risk Populations

    Colombia, South America has one of the world’s highest burdens of Helicobacter pylori infection and gastric cancer. While multidrug antibiotic regimens can effectively eradicate H. pylori, treatment efficacy is being jeopardized by the emergence of antibiotic-resistant...

    Anthony Mannion, JoAnn Dzink-Fox, Zeli Shen, M. Blanca Piazuelo, Keith T. Wilson, Pelayo Correa, Richard M. Peek, M. Constanza Camargo, James G. Fox

Mycobacteriology and Aerobic Actinomycetes

  • Open Access
    Evaluation of the Roche cobas MTB and MTB-RIF/INH Assays in Samples from Germany and Sierra Leone
    Mycobacteriology and Aerobic Actinomycetes
    Evaluation of the Roche cobas MTB and MTB-RIF/INH Assays in Samples from Germany and Sierra Leone

    The cobas MTB and MTB-RIF/INH assays allow for detection of Mycobacterium tuberculosis complex (MTBC) nucleic acid and rifampicin (RIF) and isoniazid (INH) resistance-associated mutations in an automated, high-throughput workflow. In this study, we evaluated the performance of these assays, employing samples from settings of low and high tuberculosis (TB) burdens....

    Darshaalini Nadarajan, Doris Hillemann, Rashidatu Kamara, Lynda Foray, Ousman S. Conteh, Matthias Merker, Stefan Niemann, Jasmine Lau, Merlin Njoya, Katharina Kranzer, Akos Somoskovi, Florian P. Maurer

Parasitology

  • Open Access
    Evaluation of the Performance of the Loopamp <span class="named-content genus-species" id="named-content-1">Trypanosoma cruzi</span> Detection Kit for the Diagnosis of Chagas Disease in an Area Where It Is Not Endemic, Spain
    Parasitology
    Evaluation of the Performance of the Loopamp Trypanosoma cruzi Detection Kit for the Diagnosis of Chagas Disease in an Area Where It Is Not Endemic, Spain

    In Spain, PCR is the tool of choice for the diagnosis of congenital Chagas disease (CD) and serology for diagnosing chronic CD. A loop-mediated isothermal amplification test for Trypanosoma cruzi DNA detection showed good analytical performance and ease of use.

    Maria D. Flores-Chavez, Alba Abras, Cristina Ballart, Ismael Ibáñez Perez, Pilar Perez-Gordillo, Montserrat Gállego, Carmen Muñoz, Zaira Moure, Elena Sulleiro Igual, Javier Nieto, Emilia García Diez, Israel Cruz, Albert Picado

Virology

  • Free
    Performance and Implementation Evaluation of the Abbott BinaxNOW Rapid Antigen Test in a High-Throughput Drive-Through Community Testing Site in Massachusetts
    Virology
    Performance and Implementation Evaluation of the Abbott BinaxNOW Rapid Antigen Test in a High-Throughput Drive-Through Community Testing Site in Massachusetts

    Rapid diagnostic tests (RDTs) for SARS-CoV-2 antigens (Ag) that can be performed at point of care (POC) can supplement molecular testing and help mitigate the COVID-19 pandemic. Deployment of an Ag RDT requires an understanding of its operational and performance characteristics under real-world conditions and in relevant subpopulations.

    Nira R. Pollock, Jesica R. Jacobs, Kristine Tran, Amber E. Cranston, Sita Smith, Claire Y. O’Kane, Tyler J. Roady, Anne Moran, Alison Scarry, Melissa Carroll, Leila Volinsky, Gloria Perez, Pinal Patel, Stacey Gabriel, Niall J. Lennon, Lawrence C. Madoff, Catherine Brown, Sandra C. Smole
  • Open Access
    Copan eNAT Transport System To Address Challenges in COVID-19 Diagnostics in Regions with Limited Testing Access
    Virology
    Copan eNAT Transport System To Address Challenges in COVID-19 Diagnostics in Regions with Limited Testing Access

    Community-based health care clinics and hospital outreach services have the potential to expand coronavirus disease 2019 (COVID-19) diagnostics to rural areas. However, reduced specimen stability during extended transport, the absence of a cold chain to centralized laboratories, and biosafety concerns surrounding specimen handling have limited this expansion.

    Melissa Richard-Greenblatt, Courtney E. Comar, Laurence Flevaud, Marina Berti, Rebecca M. Harris, Susan R. Weiss, Laurel Glaser
  • Free
    Evaluation of Specimen Types and Saliva Stabilization Solutions for SARS-CoV-2 Testing
    Virology
    Evaluation of Specimen Types and Saliva Stabilization Solutions for SARS-CoV-2 Testing

    Identifying SARS-CoV-2 infections through aggressive diagnostic testing remains critical to tracking and curbing the spread of the COVID-19 pandemic. Collection of nasopharyngeal swabs (NPS), the preferred sample type for SARS-CoV-2 detection, has become difficult due to the dramatic increase in testing and consequent supply strain.

    Sara B. Griesemer, Greta Van Slyke, Dylan Ehrbar, Klemen Strle, Tugba Yildirim, Dominick A. Centurioni, Anne C. Walsh, Andrew K. Chang, Michael J. Waxman, Kirsten St. George
  • Free
    Comparison of 16 Serological SARS-CoV-2 Immunoassays in 16 Clinical Laboratories
    Virology
    Comparison of 16 Serological SARS-CoV-2 Immunoassays in 16 Clinical Laboratories

    Serological assays for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are needed to support clinical diagnosis and epidemiological investigations. Recently, assays for large-scale detection of total antibodies (Ab), immunoglobulin G (IgG), and IgM against SARS-CoV-2 antigens have been developed, but there are limited data on the diagnostic accuracy of these assays.

    Lene H. Harritshøj, Mikkel Gybel-Brask, Shoaib Afzal, Pia R. Kamstrup, Charlotte S. Jørgensen, Marianne Kragh Thomsen, Linda Hilsted, Lennart Friis-Hansen, Pal B. Szecsi, Lise Pedersen, Lene Nielsen, Cecilie B. Hansen, Peter Garred, Trine-Line Korsholm, Susan Mikkelsen, Kirstine O. Nielsen, Bjarne K. Møller, Anne T. Hansen, Kasper K. Iversen, Pernille B. Nielsen, Rasmus B. Hasselbalch, Kamille Fogh, Jakob B. Norsk, Jonas Henrik Kristensen, Kristian Schønning, Nikolai S. Kirkby, Alex C. Y. Nielsen, Lone H. Landsy, Mette Loftager, Dorte K. Holm, Anna C. Nilsson, Susanne G. Sækmose, Birgitte Grum-Schwensen, Bitten Aagaard, Thøger G. Jensen, Dorte M. Nielsen, Henrik Ullum, Ram B. Dessau
  • Free
    Rapid High-Throughput Whole-Genome Sequencing of SARS-CoV-2 by Using One-Step Reverse Transcription-PCR Amplification with an Integrated Microfluidic System and Next-Generation Sequencing
    Virology
    Rapid High-Throughput Whole-Genome Sequencing of SARS-CoV-2 by Using One-Step Reverse Transcription-PCR Amplification with an Integrated Microfluidic System and Next-Generation Sequencing

    The long-lasting global COVID-19 pandemic demands timely genomic investigation of SARS-CoV-2 viruses. Here, we report a simple and efficient workflow for whole-genome sequencing utilizing one-step reverse transcription-PCR (RT-PCR) amplification on a microfluidic platform, followed by MiSeq amplicon sequencing.

    Tao Li, Hye Kyung Chung, Papa K. Pireku, Brett F. Beitzel, Mark A. Sanborn, Cynthia Y. Tang, Richard D. Hammer, Detlef Ritter, Xiu-Feng Wan, Irina Maljkovic Berry, Jun Hang
  • Free
    Portable RT-PCR System: a Rapid and Scalable Diagnostic Tool for COVID-19 Testing
    Virology
    Portable RT-PCR System: a Rapid and Scalable Diagnostic Tool for COVID-19 Testing

    Combating the ongoing coronavirus disease 2019 (COVID-19) pandemic demands accurate, rapid, and point-of-care testing with fast results to triage cases for isolation and treatment. The current testing relies on reverse transcriptase PCR (RT-PCR), which is routinely performed in well-equipped laboratories by trained professionals at specific locations.

    Hosam M. Zowawi, Thamer H. Alenazi, Waleed S. AlOmaim, Ahmad Wazzan, Abdullah Alsufayan, Rihab A. Hasanain, Omar S. Aldibasi, Sahar Althawadi, Sarah A. Altamimi, Maysoon Mutabagani, Maha Alamri, Reem S. Almaghrabi, Hail M. Al-Abdely, Ziad A. Memish, Saleh A. Alqahtani
  • Open Access
    Verification and Validation of SARS-CoV-2 Assay Performance on the Abbott <em>m</em>2000 and Alinity <em>m</em> Systems
    Virology
    Verification and Validation of SARS-CoV-2 Assay Performance on the Abbott m2000 and Alinity m Systems

    We verified the analytical performance of the Abbott RealTime SARS-CoV-2 assay on the m2000 system and compared its clinical performance to the CDC 2019-nCoV real-time PCR diagnostic panel and the Thermo Fisher TaqPath RT-PCR COVID-19 kit. We also performed a bridging study comparing the RealTime SARS-CoV-2 assay with the new Abbott Alinity m SARS-CoV-2 assay.

    Julie W. Hirschhorn, April Kegl, Tanisha Dickerson, W. Bailey Glen, Gang Xu, Jay Alden, Frederick S. Nolte
  • Deciphering an Adenovirus F41 Outbreak in Pediatric Hematopoietic Stem Cell Transplant Recipients by Whole-Genome Sequencing
    Virology
    Deciphering an Adenovirus F41 Outbreak in Pediatric Hematopoietic Stem Cell Transplant Recipients by Whole-Genome Sequencing

    Human adenovirus (HAdV) represents a major cause of mortality and morbidity in pediatric recipients of allogeneic hematopoietic stem cell transplants (HSCT). HAdV species F type 41 (HAdV-F41) infections in HSCT patients are scarce, whereas HAdV-F41 circulates commonly in healthy individuals. Between March and July 2018, HAdV-F41 infections were identified in four children (A, B, C, and E) who received allogeneic HSCT and one child...

    Caroline Lefeuvre, Maud Salmona, Linda Feghoul, Noémie Ranger, Séverine Mercier-Delarue, Laure Nizery, Aurélia Alimi, Jean-Hugues Dalle, Jérôme LeGoff
  • Open Access
    Anti-Human T-Cell Leukemia Virus Type 1 (HTLV-1) Antibody Assays in Cerebrospinal Fluid for the Diagnosis of HTLV-1-Associated Myelopathy/Tropical Spastic Paraparesis
    Virology
    Anti-Human T-Cell Leukemia Virus Type 1 (HTLV-1) Antibody Assays in Cerebrospinal Fluid for the Diagnosis of HTLV-1-Associated Myelopathy/Tropical Spastic Paraparesis

    The anti-human T-cell leukemia virus type 1 (HTLV-1) antibody assay in common use has changed from the particle agglutination (PA) method to chemiluminescent immunoassay (CLIA) and chemiluminescent enzyme immunoassay (CLEIA). These assays were validated in serum but not in cerebrospinal fluid (CSF). However, anti-HTLV-1 antibody positivity in CSF is a requisite for diagnosing HTLV-1-associated myelopathy/tropical spastic paraparesis (...

    Daisuke Kodama, Masakazu Tanaka, Toshio Matsuzaki, Satoshi Nozuma, Eiji Matsuura, Hiroshi Takashima, Shuji Izumo, Ryuji Kubota

Immunoassays

  • Impact of T-Cell Xtend on T-SPOT.<em>TB</em> Assay in High-Risk Individuals after Delayed Blood Sample Processing
    Immunoassays
    Impact of T-Cell Xtend on T-SPOT.TB Assay in High-Risk Individuals after Delayed Blood Sample Processing

    T-SPOT.TB (T-SPOT) is an interferon gamma release assay (IGRA) used to detect infection with Mycobacterium tuberculosis based on the number of spot-forming T cells; however, delays in sample processing have been shown to reduce the number of these spots that are detected following laboratory processing. Adding T-Cell Xtend (XT) into blood samples before...

    Pei-Jean Feng, Yanjue Wu, Christine S. Ho, Lance Chinna, Andrew Christian Whelen, Angela Largen, Richard Brostrom, Randall Reves, Robert Belknap, Adithya Cattamanchi, Niaz Banaei
  • Performance of Four IgM Antibody Assays in the Diagnosis of Measles Virus Primary Infection and Cases with a Serological Profile Indicating Reinfection
    Immunoassays
    Performance of Four IgM Antibody Assays in the Diagnosis of Measles Virus Primary Infection and Cases with a Serological Profile Indicating Reinfection

    The object of this study was to determine the diagnostic performance of four commercially available IgM tests in the diagnosis of measles virus (MeV) primary infection and cases with a serological profile indicating reinfection. Sera from 187 patients with MeV primary infection, 30 patients with suspected reinfection (after vaccine failure), and 153 patients with rash-like symptoms after exclusion of MeV infection were retested with...

    Georg Semmler, Stephan Walter Aberle, Hannah Griebler, Lukas Richter, Daniela Schmid, Karin Stiasny, Heidemarie Holzmann, Lukas Weseslindtner
  • Free
    Assessment of S1-, S2-, and NCP-Specific IgM, IgA, and IgG Antibody Kinetics in Acute SARS-CoV-2 Infection by a Microarray and Twelve Other Immunoassays
    Immunoassays
    Assessment of S1-, S2-, and NCP-Specific IgM, IgA, and IgG Antibody Kinetics in Acute SARS-CoV-2 Infection by a Microarray and Twelve Other Immunoassays

    In this study, we comprehensively analyzed multispecific antibody kinetics of different immunoglobulins in hospitalized patients with acute severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Three hundred fifty-four blood samples longitudinally obtained from 81 IgG-seroconverting progressed coronavirus disease 2019 (CoVID-19) patients were quantified for spike 1 (S1), S2, and nucleocapsid protein (NCP)-specific IgM...

    Georg Semmler, Marianna Theresia Traugott, Marianne Graninger, Wolfgang Hoepler, Tamara Seitz, Hasan Kelani, Mario Karolyi, Erich Pawelka, Sara Aragón de La Cruz, Elisabeth Puchhammer-Stöckl, Stephan Walter Aberle, Karin Stiasny, Alexander Zoufaly, Judith Helene Aberle, Lukas Weseslindtner

Epidemiology

  • Open Access
    Antibody Responses to Two Recombinant Treponemal Antigens (rp17 and TmpA) before and after Azithromycin Treatment for Yaws in Ghana and Papua New Guinea
    Epidemiology
    Antibody Responses to Two Recombinant Treponemal Antigens (rp17 and TmpA) before and after Azithromycin Treatment for Yaws in Ghana and Papua New Guinea

    WHO and its partners aim to interrupt yaws transmission in countries of endemicity and to certify others as being yaws-free. Transmission can be assessed using rapid plasma reagin (RPR) tests, reflecting current or recent infection, but RPR is operationally impractical.

    Nishanth Parameswaran, Oriol Mitjà, Christian Bottomley, Cynthia Kwakye, Wendy Houinei, Allan Pillay, Damien Danavall, Kai-Hua Chi, Ronald C. Ballard, Anthony W. Solomon, Cheng Y. Chen, Sibauk V. Bieb, Yaw Adu-Sarkodie, David C. W. Mabey, Kingsley Asiedu, Michael Marks, Diana L. Martin
  • Open Access
    Evaluating Specimen Quality and Results from a Community-Wide, Home-Based Respiratory Surveillance Study
    Epidemiology
    Evaluating Specimen Quality and Results from a Community-Wide, Home-Based Respiratory Surveillance Study

    While influenza and other respiratory pathogens cause significant morbidity and mortality, the community-based burden of these infections remains incompletely understood. The development of novel methods to detect respiratory infections is essential for mitigating epidemics and developing pandemic-preparedness infrastructure.

    Ashley E. Kim, Elisabeth Brandstetter, Naomi Wilcox, Jessica Heimonen, Chelsey Graham, Peter D. Han, Lea M. Starita, Denise J. McCulloch, Amanda M. Casto, Deborah A. Nickerson, Margaret M. Van de Loo, Jennifer Mooney, Misja Ilcisin, Kairsten A. Fay, Jover Lee, Thomas R. Sibley, Victoria Lyon, Rachel E. Geyer, Matthew Thompson, Barry R. Lutz, Mark J. Rieder, Trevor Bedford, Michael Boeckh, Janet A. Englund, Helen Y. Chu
    and on behalf of the Seattle Flu Study Investigators
  • Open Access
    <span class="named-content genus-species" id="named-content-1">Mycoplasma hyopneumoniae</span> Surveillance in Pig Populations: Establishing Sampling Guidelines for Detection in Growing Pigs
    Epidemiology
    Mycoplasma hyopneumoniae Surveillance in Pig Populations: Establishing Sampling Guidelines for Detection in Growing Pigs

    Antemortem detection of Mycoplasma hyopneumoniae infection in swine production systems has relied on antibody testing, but the availability of tests based on DNA detection and novel diagnostic specimens, e.g., tracheal swabs and oral fluids, has the potential to improve M. hyopneumoniae surveillance...

    Maria Jose Clavijo, Dapeng Hu, Seth Krantz, Jean Paul Cano, Thairê Pereira Maróstica, Alexandra Henao-Diaz, Ana Paula S. Poeta Silva, Deanne Hemker, Edgar Tapia, Silvia Zimmerman, Eduardo Fano, Dale Polson, Robert Fitzgerald, Alexander Tucker, Rodger Main, Chong Wang, Jeffrey J. Zimmerman, Marisa L. Rotolo

Letters to the Editor

  • Taxonomy Revision of <em>Leishmania</em> spp. in Thailand
    Letter to the Editor
    Taxonomy Revision of Leishmania spp. in Thailand
    Mathirut Mungthin, Saovanee Leelayoova, Suradej Siripattanapipong
  • Free
    Evaluation of Performance of the BD Veritor SARS-CoV-2 Chromatographic Immunoassay Test in Patients with Symptoms of COVID-19
    Letter to the Editor
    Evaluation of Performance of the BD Veritor SARS-CoV-2 Chromatographic Immunoassay Test in Patients with Symptoms of COVID-19
    Abdullah Kilic, Brian Hiestand, Elizabeth Palavecino
  • New Pneumococcal Serotype 15D
    Letter to the Editor
    New Pneumococcal Serotype 15D
    Fabiana Pimenta, Benild Moiane, Robert E. Gertz, Sopio Chochua, Paula M. Snippes Vagnone, Ruth Lynfield, Betuel Sigaúque, Maria da Gloria Carvalho, Bernard Beall
  • Reply to Mungthin et al., “Taxonomy Revision of <em>Leishmania</em> spp. in Thailand”
    Letter to the Editor
    Reply to Mungthin et al., “Taxonomy Revision of Leishmania spp. in Thailand”
    Blaine A. Mathison, Richard S. Bradbury, Bobbi S. Pritt
  • Open Access
    Comparable Specimen Collection from Both Ends of At-Home Midturbinate Swabs
    Letter to the Editor
    Comparable Specimen Collection from Both Ends of At-Home Midturbinate Swabs
    Melissa Truong, Brian Pfau, Evan McDermot, Peter D. Han, Elisabeth Brandstetter, Matthew Richardson, Ashley E. Kim, Mark J. Rieder, Helen Y. Chu, Janet A. Englund, Deborah A. Nickerson, Jay Shendure, Christina M. Lockwood, Eric Q. Konnick, Lea M. Starita
    and on behalf of the Seattle Flu Study Investigators
  • Open Access
    Multicenter Evaluation of Processing and Analysis of College of American Pathologists (CAP) Proficiency Testing Samples by Laboratory Automation
    Letter to the Editor
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