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Journal of Clinical Microbiology
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Advanced Search

  • Home
  • Articles
    • Current Issue
    • Accepted Manuscripts
    • COVID-19 Special Collection
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    • Minireviews
  • For Authors
    • Submit a Manuscript
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  • Isolation and Immunogenicity Analysis of Porcine Epidemic Diarrhea Virus
    Isolation and Immunogenicity Analysis of Porcine Epidemic Diarrhea Virus
  • Deep Learning Model for Diagnosis of Bacterial Vaginosis
    Deep Learning Model for Diagnosis of Bacterial Vaginosis
  • 1
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  • Current Issue
  • Accepted Manuscripts
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Photo Quiz

  • Photo Quiz: A 54-Year-Old Congolese Woman with Knee Pain
    Photo Quiz
    Photo Quiz: A 54-Year-Old Congolese Woman with Knee Pain
    Damien Costa, Pascal Rottenberg, Emmanuel Toure, Pascal Delaunay, Loic Favennec, Gilles Gargala
  • Answer to January 2021 Photo Quiz
    Photo Quiz
    Answer to January 2021 Photo Quiz
    Damien Costa, Pascal Rottenberg, Emmanuel Toure, Pascal Delaunay, Loic Favennec, Gilles Gargala

Editorial

  • Free
    When Should Asymptomatic Persons Be Tested for COVID-19?
    Editorial
    When Should Asymptomatic Persons Be Tested for COVID-19?

    On 24 August 2020, the Centers for Disease Control and Prevention (CDC) updated its website to highlight that asymptomatic individuals, even those with exposure to a COVID-19-positive contact, do not necessarily need to be tested unless they have medical conditions associated with increased risk of severe illness from COVID-19. The CDC subsequently updated its guidance on 19 September 2020 to support testing of asymptomatic persons,...

    Audrey N. Schuetz, Peera Hemarajata, Ninad Mehta, Sheldon Campbell, Stephanie Mitchell, Elizabeth Palavecino, Susan Butler-Wu, Melissa B. Miller

The Brief Case

  • Free
    The Brief Case: <em>Loa loa</em> in a Patient from Nigeria
    The Brief Case
    The Brief Case: Loa loa in a Patient from Nigeria
    Eileen M. Burd, Ahmed Babiker, Jessica K. Fairley, Divya Bhamidipati, Laila E. Woc-Colburn, Blaine A. Mathison
  • Free
    Closing the Brief Case: <em>Loa loa</em> in a Patient from Nigeria
    The Brief Case
    Closing the Brief Case: Loa loa in a Patient from Nigeria
    Eileen M. Burd, Ahmed Babiker, Jessica K. Fairley, Divya Bhamidipati, Laila E. Woc-Colburn, Blaine A. Mathison

Commentary

  • Free
    Application, Verification, and Implementation of SARS-CoV-2 Serologic Assays with Emergency Use Authorization
    Commentary
    Application, Verification, and Implementation of SARS-CoV-2 Serologic Assays with Emergency Use Authorization

    Interest continues to grow regarding the role of serologic assays for the detection of prior infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The U.S. Food and Drug Administration (FDA) has granted emergency use authorization (EUA) status to many SARS-CoV-2 serologic assays. In this document, expert recommendations from clinical microbiologist members of the American Society for Microbiology (ASM) concerning...

    Elitza S. Theel, Marc Roger Couturier, Laura Filkins, Elizabeth Palavecino, Stephanie Mitchell, Sheldon Campbell, Michael Pentella, Susan Butler-Wu, Kurt Jerke, Vaishali Dharmarha, Peggy McNult, Audrey N. Schuetz
    and on behalf of the American Society for Microbiology Clinical and Public Health Microbiology Committee, Subcommittee on Laboratory Practices

Minireview

  • Cefiderocol Antimicrobial Susceptibility Testing Considerations: the Achilles' Heel of the Trojan Horse?
    Minireview
    Cefiderocol Antimicrobial Susceptibility Testing Considerations: the Achilles' Heel of the Trojan Horse?

    Cefiderocol (formerly S-649266) is a novel siderophore-conjugated cephalosporin with activity against a broad array of multidrug-resistant (MDR), aerobic Gram-negative bacilli. The siderophore component binds iron and uses active iron transport for drug entry into the bacterial periplasmic space. The cephalosporin moiety is the active antimicrobial component, structurally resembling a hybrid between ceftazidime and cefepime. Like other...

    Patricia J. Simner, Robin Patel

Bacteriology

  • Molecular Microbiological and Immune Characterization of a Cohort of Patients Diagnosed with Early Lyme Disease
    Bacteriology
    Molecular Microbiological and Immune Characterization of a Cohort of Patients Diagnosed with Early Lyme Disease

    Lyme disease is a tick-borne infection caused by the bacteria Borrelia burgdorferi. Current diagnosis of early Lyme disease relies heavily on clinical criteria, including the presence of an erythema migrans rash. The sensitivity of current gold-standard diagnostic tests relies upon antibody formation, which is typically delayed and thus of limited utility in early...

    Michael R. Mosel, Alison W. Rebman, Heather E. Carolan, Tristan Montenegro, Robert Lovari, Steven E. Schutzer, David J. Ecker, Ting Yang, Nitya S. Ramadoss, William H. Robinson, Mark J. Soloski, Mark W. Eshoo, John N. Aucott
  • Open Access
    Comparison of Agar Dilution to Broth Microdilution for Testing <em>In Vitro</em> Activity of Cefiderocol against Gram-Negative Bacilli
    Bacteriology
    Comparison of Agar Dilution to Broth Microdilution for Testing In Vitro Activity of Cefiderocol against Gram-Negative Bacilli

    Cefiderocol (CFDC) is a siderophore cephalosporin with activity against Gram-negative bacterial species that are resistant to carbapenems and other drugs. The MICs of CFDC were determined for 610 Gram-negative bacilli, including 302 multinational Enterobacterales isolates with characterized mechanisms of beta-lactam resistance, 180 clinical isolates from the Mayo Clinic and Mayo Clinic Laboratories not characterized for...

    Mariana Albano, Melissa J. Karau, Audrey N. Schuetz, Robin Patel
  • Multicenter Performance Evaluation of the Simplexa Bordetella Direct Kit in Nasopharyngeal Swab Specimens
    Bacteriology
    Multicenter Performance Evaluation of the Simplexa Bordetella Direct Kit in Nasopharyngeal Swab Specimens

    Detection of Bordetella pertussis and Bordetella parapertussis using molecular methods is sensitive and specific with a short turnaround time compared to other diagnostic methods. In this multicenter study, we compared the performance of the Simplexa Bordetella Direct kit to those of other molecular...

    Siu-Kei Chow, Sophie Arbefeville, Bobby L. Boyanton, Emily M. Dault, James Dunn, Patricia Ferrieri, Wallace Greene, Morgan A. Pence, Joshua Otiso, Sandra Richter, Ted E. Schutzbank
  • Evaluation of the Biotoxis qPCR Detection Kit for <span class="named-content genus-species" id="named-content-1">Francisella tularensis</span> Detection in Clinical and Environmental Samples
    Bacteriology
    Evaluation of the Biotoxis qPCR Detection Kit for Francisella tularensis Detection in Clinical and Environmental Samples

    Rapid and reliable detection and identification of Francisella tularensis (a tier 1 select agent) are of primary interest for both medical and biological threat surveillance purposes. The Biotoxis qPCR detection kit is a real-time quantitative PCR (qPCR) assay designed for the detection of Bacillus...

    Aurélie Hennebique, Fabienne Gas, Hélène Batina, Cécilia De Araujo, Karine Bizet, Max Maurin
  • Cefiderocol Antimicrobial Susceptibility Testing against Multidrug-Resistant Gram-Negative Bacilli: a Comparison of Disk Diffusion to Broth Microdilution
    Bacteriology
    Cefiderocol Antimicrobial Susceptibility Testing against Multidrug-Resistant Gram-Negative Bacilli: a Comparison of Disk Diffusion to Broth Microdilution

    Antimicrobial susceptibility testing (AST) of cefiderocol poses challenges because of its unique mechanism of action (i.e., requiring an iron-depleted state) and due to differences in interpretative criteria established by the Clinical and Laboratory Standards Institute (CLSI), U.S. Food and Drug Administration (FDA), and European Committee on Antimicrobial Susceptibility Testing (EUCAST). Our objective was to compare cefiderocol disk...

    C. Paul Morris, Yehudit Bergman, Tsigedera Tekle, John A. Fissel, Pranita D. Tamma, Patricia J. Simner
  • Simple Phenotypic Tests To Improve Accuracy in Screening Chromosomal and Plasmid-Mediated Colistin Resistance in Gram-Negative Bacilli
    Bacteriology
    Simple Phenotypic Tests To Improve Accuracy in Screening Chromosomal and Plasmid-Mediated Colistin Resistance in Gram-Negative Bacilli

    CLSI and EUCAST recommend that only broth microdilution (BMD) should be used for routine colistin susceptibility testing; however, this technique can be difficult to perform in resource-poor settings. The purpose of this study was to evaluate the accuracy of a colistin agar spot test (COL-AS) and a colistin drop test (COL-DT) compared to BMD. COL-AS and COL-DT were assessed with a collection of 271 Gram-negative bacilli clinical...

    Fernando Pasteran, Diego Danze, Alejandra Menocal, Carla Cabrera, Ignacio Castillo, Ezequiel Albornoz, Celeste Lucero, Melina Rapoport, Paola Ceriana, Alejandra Corso
  • Open Access
    Sequential Quadriplex Real-Time PCR for Identifying 20 Common <em>emm</em> Types of Group A <em>Streptococcus</em>
    Bacteriology
    Sequential Quadriplex Real-Time PCR for Identifying 20 Common emm Types of Group A Streptococcus

    We developed a sequential quadriplex real-time PCR-based method for rapid identification of 20 emm types commonly found in invasive group A Streptococcus (iGAS) strains recovered through the Centers for Disease Control and Prevention’s Active Bacterial Core surveillance. Each emm real-time PCR assay showed high specificity and accurately identified the respective target emm type, including emm...

    Srinivasan Velusamy, Katherine Jordak, Madeline Kupor, Sopio Chochua, Lesley McGee, Bernard Beall
  • Open Access
    Digital Image Analysis for the Detection of Group B <em>Streptococcus</em> from ChromID Strepto B Medium Using PhenoMatrix Algorithms
    Bacteriology
    Digital Image Analysis for the Detection of Group B Streptococcus from ChromID Strepto B Medium Using PhenoMatrix Algorithms

    Group B Streptococcus (GBS) can be found to colonize about 25% of all healthy, adult women and is the leading infectious cause of early neonatal morbidity and mortality in the United States. This study evaluated the clinical performance of PhenoMatrix (PM) chromogenic detection module (CDM) digital imaging software in detection of GBS from LIM broth plated on ChromID Strepto B chromogenic medium (ChromID) using the WASP...

    Justin Baker, Karen Timm, Matthew Faron, Nathan Ledeboer, Karissa Culbreath
  • Open Access
    Evaluation of Pneumococcal Serotyping of Nasopharyngeal-Carriage Isolates by Latex Agglutination, Whole-Genome Sequencing (PneumoCaT), and DNA Microarray in a High-Pneumococcal-Carriage-Prevalence Population in Malawi
    Bacteriology
    Evaluation of Pneumococcal Serotyping of Nasopharyngeal-Carriage Isolates by Latex Agglutination, Whole-Genome Sequencing (PneumoCaT), and DNA Microarray in a High-Pneumococcal-Carriage-Prevalence Population in Malawi

    Accurate assessment of the serotype distribution associated with pneumococcal colonization and disease is essential for evaluating and formulating pneumococcal vaccines and for informing vaccine policy. For this reason, we evaluated the concordance between pneumococcal serotyping results by latex agglutination, whole-genome sequencing (WGS) with PneumoCaT, and DNA microarray for samples from community carriage surveillance in Blantyre,...

    Todd D. Swarthout, Andrea Gori, Naor Bar-Zeev, Arox W. Kamng’ona, Thandie S. Mwalukomo, Farouck Bonomali, Roseline Nyirenda, Comfort Brown, Jacquline Msefula, Dean Everett, Charles Mwansambo, Katherine Gould, Jason Hinds, Robert S. Heyderman, Neil French
  • Open Access
    Meningococcal Deduced Vaccine Antigen Reactivity (MenDeVAR) Index: a Rapid and Accessible Tool That Exploits Genomic Data in Public Health and Clinical Microbiology Applications
    Bacteriology
    Meningococcal Deduced Vaccine Antigen Reactivity (MenDeVAR) Index: a Rapid and Accessible Tool That Exploits Genomic Data in Public Health and Clinical Microbiology Applications

    As microbial genomics makes increasingly important contributions to clinical and public health microbiology, the interpretation of whole-genome sequence data by nonspecialists becomes essential. In the absence of capsule-based vaccines, two protein-based vaccines have been used for the prevention of invasive serogroup B meningococcal disease (IMD) since their licensure in 2013 and 2014. These vaccines have different components and...

    Charlene M. C. Rodrigues, Keith A. Jolley, Andrew Smith, J. Claire Cameron, Ian M. Feavers, Martin C. J. Maiden
  • Evaluation of Surrogate Tests for the Presence of <em>mecA</em>-Mediated Methicillin Resistance in <span class="named-content genus-species" id="named-content-1">Staphylococcus capitis</span>, <span class="named-content genus-species" id="named-content-2">Staphylococcus haemolyticus</span>, <span class="named-content genus-species" id="named-content-3">Staphylococcus hominis</span>, and <span class="named-content genus-species" id="named-content-4">Staphylococcus warneri</span>
    Bacteriology
    Evaluation of Surrogate Tests for the Presence of mecA-Mediated Methicillin Resistance in Staphylococcus capitis, Staphylococcus haemolyticus, Staphylococcus hominis, and Staphylococcus warneri

    Testing of staphylococci other than Staphylococcus aureus (SOSA) for mecA-mediated resistance is challenging. Isolates of Staphylococcus capitis, Staphylococcus haemolyticus,...

    Romney M. Humphries, Paul Magnano, Carey-Ann D. Burnham, Jennifer Dien Bard, Tanis C. Dingle, Katrina Callan, Lars F. Westblade
    and the Coagulase Negative Staphylococcus Ad Hoc Working Group
  • Novel Strategy for Rapidly and Safely Distinguishing <span class="named-content genus-species" id="named-content-1">Bacillus anthracis</span> and <span class="named-content genus-species" id="named-content-2">Bacillus cereus</span> by Use of Peptide Mass Fingerprints Based on Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry
    Bacteriology
    Novel Strategy for Rapidly and Safely Distinguishing Bacillus anthracis and Bacillus cereus by Use of Peptide Mass Fingerprints Based on Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry

    The objective of this study was to construct a rapid, high-throughput, and biosafety-compatible screening method for Bacillus anthracis and Bacillus cereus based on matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS). MALDI-TOF MS coupled to ClinProTools was...

    Jianchun Wei, Huijuan Zhang, Huifang Zhang, Enmin Zhang, Binghua Zhang, Fei Zhao, Di Xiao

Mycobacteriology and Aerobic Actinomycetes

  • Assessing Nanopore Sequencing for Clinical Diagnostics: a Comparison of Next-Generation Sequencing (NGS) Methods for <span class="named-content genus-species" id="named-content-1">Mycobacterium tuberculosis</span>
    Mycobacteriology and Aerobic Actinomycetes
    Assessing Nanopore Sequencing for Clinical Diagnostics: a Comparison of Next-Generation Sequencing (NGS) Methods for Mycobacterium tuberculosis

    Next-generation sequencing technologies are being rapidly adopted as a tool of choice for diagnostic and outbreak investigation in public health laboratories. However, costs of operation and the need for specialized staff remain major hurdles for laboratories with limited resources for implementing these technologies. This project aimed to assess the feasibility of using Oxford Nanopore MinION whole-genome sequencing data of...

    Carol Smith, Tanya A. Halse, Joseph Shea, Herns Modestil, Randal C. Fowler, Kimberlee A. Musser, Vincent Escuyer, Pascal Lapierre

Mycology

  • Free
    COVID-19-Associated Invasive Aspergillosis: Data from the UK National Mycology Reference Laboratory
    Mycology
    COVID-19-Associated Invasive Aspergillosis: Data from the UK National Mycology Reference Laboratory

    COVID-19-associated pulmonary aspergillosis (CAPA) was recently reported as a potential infective complication affecting critically ill patients with acute respiratory distress syndrome following severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, with incidence rates varying from 8 to 33% depending on the study. However, definitive diagnosis of CAPA is challenging. Standardized diagnostic algorithms and definitions...

    Andrew M. Borman, Michael D. Palmer, Mark Fraser, Zoe Patterson, Ciara Mann, Debra Oliver, Christopher J. Linton, Martin Gough, Phillipa Brown, Agnieszka Dzietczyk, Michelle Hedley, Sue McLachlan, Julie King, Elizabeth M. Johnson
  • Open Access
    Diagnostic Accuracy of the Biosynex CryptoPS Cryptococcal Antigen Semiquantitative Lateral Flow Assay in Patients with Advanced HIV Disease
    Mycology
    Diagnostic Accuracy of the Biosynex CryptoPS Cryptococcal Antigen Semiquantitative Lateral Flow Assay in Patients with Advanced HIV Disease

    High cryptococcal antigen (CrAg) titers in blood are associated with subclinical meningitis and mortality in CrAg-positive individuals with advanced HIV disease (AHD). We evaluated a novel semiquantitative lateral flow assay (LFA), CryptoPS, that may be able to identify individuals with high CrAg titers in a cohort of AHD patients undergoing CrAg screening. In a prospective cohort of patients with AHD (CD4 cell count, ≤200/μl) receiving...

    Mark W. Tenforde, Timothée Boyer-Chammard, Charles Muthoga, Leabaneng Tawe, Thandi Milton, Ikanyeng Rulaganyang, Kwana Lechiile, Ivy Rukasha, Tshepo B. Leeme, Nelesh P. Govender, Julia Ngidi, Madisa Mine, Síle F. Molloy, Thomas S. Harrison, Olivier Lortholary, Joseph N. Jarvis

Parasitology

  • A Real-Time PCR Assay for Simultaneous Detection and Differentiation of Four Common <em>Entamoeba</em> Species That Infect Humans
    Parasitology
    A Real-Time PCR Assay for Simultaneous Detection and Differentiation of Four Common Entamoeba Species That Infect Humans

    There are over 40 species within the genus Entamoeba, eight of which infect humans. Of these, four species (Entamoeba histolytica, E. dispar, E. moshkovskii, and E. bangladeshi) are morphologically indistinguishable from each other, and yet differentiation is important for appropriate treatment decisions. Here, we developed a...

    Ibne Karim M. Ali, Shantanu Roy

Virology

  • Open Access
    Performance Evaluation of the SAMBA II SARS-CoV-2 Test for Point-of-Care Detection of SARS-CoV-2
    Virology
    Performance Evaluation of the SAMBA II SARS-CoV-2 Test for Point-of-Care Detection of SARS-CoV-2

    Nucleic acid amplification for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA in respiratory samples is the standard method for diagnosis. The majority of this testing is centralized and therefore has turnaround times of several days. Point-of-care (POC) testing with rapid turnaround times would allow more effective triage in settings where patient management and infection control decisions need to be...

    Sonny M. Assennato, Allyson V. Ritchie, Cesar Nadala, Neha Goel, Cuijuan Tie, Lourdes M. Nadala, Hongyi Zhang, Rawlings Datir, Ravindra K. Gupta, Martin D. Curran, Helen H. Lee
  • Diagnostic Accuracy of Four Commercial Triplex Immunochromatographic Tests for Rapid Detection of Rotavirus, Adenovirus, and Norovirus in Human Stool Samples
    Virology
    Diagnostic Accuracy of Four Commercial Triplex Immunochromatographic Tests for Rapid Detection of Rotavirus, Adenovirus, and Norovirus in Human Stool Samples

    Noroviruses (NoV), rotaviruses (RVA), and adenoviruses (AdV) are the main viral agents responsible for acute gastroenteritis (AGE) in humans. We aimed to determine the diagnostic accuracy of four commercial immunochromatographic tests (ICTs) intended for the rapid and simultaneous detection of these three pathogens. Diagnostic accuracy of bioNexia Noro/Rota-Adeno (bioMérieux), Immunoquick NoRotAdeno (Biosynex), Rota+Adeno+Noro combo...

    Jérôme Kaplon, Lucie Théry, Maxime Bidalot, Nadège Grangier, Jean Frappier, Ludwig Serge Aho Glélé, Alexis de Rougemont, Katia Ambert-Balay
  • Free
    Evaluation of Three Commercial SARS-CoV-2 Serologic Assays and Their Performance in Two-Test Algorithms
    Virology
    Evaluation of Three Commercial SARS-CoV-2 Serologic Assays and Their Performance in Two-Test Algorithms

    Sensitive and specific severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) serologic assays are needed to inform diagnostic, therapeutic, and public health decision-making. We evaluated three commercial serologic assays as stand-alone tests and as components of two-test algorithms. Two nucleocapsid antibody tests (Abbott IgG and Roche total antibody) and one spike protein antibody test (DiaSorin IgG) were included. We assessed...

    Sarah E. Turbett, Melis Anahtar, Anand S. Dighe, Wilfredo Garcia Beltran, Tyler Miller, Hannah Scott, Sienna Marie Durbin, Maheetha Bharadwaj, Jason Thomas, Tasos S. Gogakos, Michael Astudillo, Jochen Lennerz, Eric S. Rosenberg, John A. Branda
  • Open Access
    Analytical and Clinical Sample Performance Characteristics of the Onclarity Assay for the Detection of Human Papillomavirus
    Virology
    Analytical and Clinical Sample Performance Characteristics of the Onclarity Assay for the Detection of Human Papillomavirus

    The objective of this study was to determine the result reproducibility and performance of the BD Onclarity human papillomavirus (HPV) assay (Onclarity) on the BD Viper LT platform using both contrived and clinical specimens. Reproducibility was assessed in BD SurePath liquid-based cytology (LBC) medium (SurePath) using contrived panels (HPV genotype 16 [HPV16] positive, HPV18 positive, or HPV45 positive) or clinical specimens (HPV16, -...

    Stephen Young, Laurence Vaughan, Karen Yanson, Karen Eckert, Aojun Li, James Harris, Aaron Ermel, James A. Williams, Mohammad Al-Ghoul, Catherine L. Cammarata, Stephanie N. Taylor, Ronald Luff, Charles K. Cooper, Barbara Van Der Pol
  • Positive Impact of Routine Testing for Enterovirus and Parechovirus on Length of Hospitalization and Antimicrobial Use among Inpatients ≤6 Months of Age
    Virology
    Positive Impact of Routine Testing for Enterovirus and Parechovirus on Length of Hospitalization and Antimicrobial Use among Inpatients ≤6 Months of Age

    Enterovirus (EV) and parechovirus (PeV) are leading viral causes of central nervous system (CNS) infection among hospitalized neonates and young infants, yet testing for PeV is not routinely performed. The goal of our study was to determine how EV and PeV CSF RT-PCR testing impacted the duration of antibiotic use and the length of hospitalization (LOS) in children ≤6 months old with suspected CNS infection. This retrospective cohort...

    Brian R. Lee, Anjana Sasidharan, Chris J. Harrison, Rangaraj Selvarangan
  • Free
    Metagenomic Sequencing To Detect Respiratory Viruses in Persons under Investigation for COVID-19
    Virology
    Metagenomic Sequencing To Detect Respiratory Viruses in Persons under Investigation for COVID-19

    Broad testing for respiratory viruses among persons under investigation (PUIs) for SARS-CoV-2 has been performed inconsistently, limiting our understanding of alternative viral infections and coinfections in these patients. RNA metagenomic next-generation sequencing (mNGS) offers an agnostic tool for the detection of both SARS-CoV-2 and other RNA respiratory viruses in PUIs. Here, we used RNA mNGS to assess the frequencies of...

    Ahmed Babiker, Heath L. Bradley, Victoria D. Stittleburg, Jessica M. Ingersoll, Autum Key, Colleen S. Kraft, Jesse J. Waggoner, Anne Piantadosi
  • A Clinical Epidemiology and Molecular Attribution Evaluation of Adenoviruses in Pediatric Acute Gastroenteritis: a Case-Control Study
    Virology
    A Clinical Epidemiology and Molecular Attribution Evaluation of Adenoviruses in Pediatric Acute Gastroenteritis: a Case-Control Study

    The objective of this study was to characterize the etiological role of human adenovirus (HAdV) serotypes in pediatric gastroenteritis. Using a case-control design, we compared the frequencies of HAdV serotypes between children with ≥3 episodes of vomiting or diarrhea within 24 h and <7 days of symptoms (i.e., cases) and those with no infectious symptoms (i.e., controls). Stool samples and/or rectal swabs underwent molecular...

    Kanti Pabbaraju, Raymond Tellier, Xiao-Li Pang, Jianling Xie, Bonita E. Lee, Linda Chui, Ran Zhuo, Otto G. Vanderkooi, Samina Ali, Phillip I. Tarr, Anna Funk, Stephen B. Freedman
    and on behalf of the Alberta Provincial Pediatric EnTeric Infection TEam (APPETITE)
  • HIV-2 Drug Resistance Genotyping from Dried Blood Spots
    Virology
    HIV-2 Drug Resistance Genotyping from Dried Blood Spots

    The treatment of HIV-2 in resource-limited settings (RLS) is complicated by the limited availability of HIV-2-active antiretroviral drugs and inadequate access to HIV-2 viral load and drug resistance testing. Dried blood spots (DBS)-based drug resistance testing, widely studied for HIV-1, has not been reported for HIV-2 and could present an opportunity to improve care for HIV-2-infected individuals. We selected 150 DBS specimens from...

    Dana N. Raugi, Robert S. Nixon, Sally Leong, Khadim Faye, Jean Phillipe Diatta, Fatima Sall, Robert A. Smith, ElHadji Ibrahima Sall, Jean Jacques Malomar, Moussa Seydi, Geoffrey S. Gottlieb
    and for the University of Washington-Senegal HIV-2 Research Group
  • Open Access
    Clinical Evaluation of BD Veritor SARS-CoV-2 Point-of-Care Test Performance Compared to PCR-Based Testing and versus the Sofia 2 SARS Antigen Point-of-Care Test
    Virology
    Clinical Evaluation of BD Veritor SARS-CoV-2 Point-of-Care Test Performance Compared to PCR-Based Testing and versus the Sofia 2 SARS Antigen Point-of-Care Test

    The clinical performance of the BD Veritor System for Rapid Detection of SARS-CoV-2 nucleocapsid antigen (Veritor), a chromatographic immunoassay used for SARS-CoV-2 point-of-care testing, was evaluated using nasal specimens from individuals with COVID-19 symptoms. Two studies were completed to determine clinical performance. In the first study, nasal specimens and either nasopharyngeal or oropharyngeal specimens from 251 participants...

    Stephen Young, Stephanie N. Taylor, Catherine L. Cammarata, Katey G. Varnado, Celine Roger-Dalbert, Amanda Montano, Christen Griego-Fullbright, Cameron Burgard, Catherine Fernandez, Karen Eckert, Jeffrey C. Andrews, Huimiao Ren, Joseph Allen, Ronald Ackerman, Charles K. Cooper
  • Free
    Comparison of Upper Respiratory Viral Load Distributions in Asymptomatic and Symptomatic Children Diagnosed with SARS-CoV-2 Infection in Pediatric Hospital Testing Programs
    Virology
    Comparison of Upper Respiratory Viral Load Distributions in Asymptomatic and Symptomatic Children Diagnosed with SARS-CoV-2 Infection in Pediatric Hospital Testing Programs

    The distribution of upper respiratory viral loads (VL) in asymptomatic children infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is unknown. We assessed PCR cycle threshold (Ct) values and estimated VL in infected asymptomatic children diagnosed in nine pediatric hospital testing programs. Records for asymptomatic and symptomatic patients with positive clinical SARS-CoV-2 tests were reviewed. Ct values were (i...

    Larry K. Kociolek, William J. Muller, Rebecca Yee, Jennifer Dien Bard, Cameron A. Brown, Paula A. Revell, Hanna Wardell, Timothy J. Savage, Sarah Jung, Samuel Dominguez, Bijal A. Parikh, Robert C. Jerris, Sue C. Kehl, Aaron Campigotto, Jeffrey M. Bender, Xiaotian Zheng, Emily Muscat, Matthew Linam, Lisa Abuogi, Christiana Smith, Kelly Graff, Ariel Hernandez-Leyva, David Williams, Nira R. Pollock

Immunoassays

  • Free
    Comparison of the Clinical Performances of the Abbott Alinity IgG, Abbott Architect IgM, and Roche Elecsys Total SARS-CoV-2 Antibody Assays
    Immunoassays
    Comparison of the Clinical Performances of the Abbott Alinity IgG, Abbott Architect IgM, and Roche Elecsys Total SARS-CoV-2 Antibody Assays

    Critical evaluation of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) serologic assays is needed to guide clinical decision-making and ensure that these assays provide optimal benefit to patients and the public. Here, three commercially available assays with widespread distribution capabilities are compared. A total of 667 specimens, 103 from patients with confirmed SARS-CoV-2 infections and 564 collected prior to the...

    Kellisha Harley, Ian L. Gunsolus
  • Free
    COVID-19 Serology at Population Scale: SARS-CoV-2-Specific Antibody Responses in Saliva
    Immunoassays
    COVID-19 Serology at Population Scale: SARS-CoV-2-Specific Antibody Responses in Saliva

    Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the cause of an ongoing pandemic that has infected over 36 million and killed over 1 million people. Informed implementation of government public health policies depends on accurate data on SARS-CoV-2 immunity at a population scale. We hypothesized that detection of SARS-CoV-2 salivary antibodies could serve as a noninvasive alternative to serological testing for monitoring...

    Nora Pisanic, Pranay R. Randad, Kate Kruczynski, Yukari C. Manabe, David L. Thomas, Andrew Pekosz, Sabra L. Klein, Michael J. Betenbaugh, William A. Clarke, Oliver Laeyendecker, Patrizio P. Caturegli, H. Benjamin Larman, Barbara Detrick, Jessica K. Fairley, Amy C. Sherman, Nadine Rouphael, Srilatha Edupuganti, Douglas A. Granger, Steve W. Granger, Matthew H. Collins, Christopher D. Heaney

Clinical Veterinary Microbiology

  • Open Access
    Evaluation of a Fecal Shedding Test To Detect Badger Social Groups Infected with <span class="named-content genus-species" id="named-content-1">Mycobacterium bovis</span>
    Clinical Veterinary Microbiology
    Evaluation of a Fecal Shedding Test To Detect Badger Social Groups Infected with Mycobacterium bovis

    Bovine tuberculosis (bTB) is an economically important disease affecting the cattle industry in England and Wales. bTB, caused by Mycobacterium bovis, also causes disease in the Eurasian badger (Meles meles), a secondary maintenance host. Disease transmission between these two species is...

    Andrew R. J. Murphy, Emma R. Travis, Victoria Hibberd, David Porter, Elizabeth M. H. Wellington

Epidemiology

  • Open Access
    Global Molecular Epidemiology of Respiratory Syncytial Virus from the 2017−2018 INFORM-RSV Study
    Epidemiology
    Global Molecular Epidemiology of Respiratory Syncytial Virus from the 2017−2018 INFORM-RSV Study

    Respiratory syncytial virus (RSV) is the leading cause of lower respiratory tract infection among infants and young children, resulting in annual epidemics worldwide. INFORM-RSV is a multiyear clinical study designed to describe the global molecular epidemiology of RSV in children under 5 years of age by monitoring temporal and geographical evolution of current circulating RSV strains, F protein antigenic sites, and their relationships...

    David E. Tabor, Fiona Fernandes, Annefleur C. Langedijk, Deidre Wilkins, Robert Jan Lebbink, Andrey Tovchigrechko, Alexey Ruzin, Leyla Kragten-Tabatabaie, Hong Jin, Mark T. Esser, Louis J. Bont, Michael E. Abram, the INFORM-RSV Study Group
  • C-Reactive Protein Testing for Active Tuberculosis among Inpatients without HIV in Uganda: a Diagnostic Accuracy Study
    Epidemiology
    C-Reactive Protein Testing for Active Tuberculosis among Inpatients without HIV in Uganda: a Diagnostic Accuracy Study

    The objective of this prospective cross-sectional study, conducted at a national referral hospital in Kampala, Uganda, was to determine diagnostic performance of serum C-reactive protein (CRP) as a triage test for tuberculosis (TB) among HIV-seronegative inpatients. We calculated the sensitivity, specificity, positive and negative likelihood ratios, and positive and negative predictive values to determine the diagnostic performance of a...

    Amanda J. Meyer, Emmanuel Ochom, Patricia Turimumahoro, Patrick Byanyima, Ingvar Sanyu, Rejani Lalitha, Sylvia Kaswabuli, Alfred Andama, Nicholas D. Walter, Achilles Katamba, Adithya Cattamanchi, William Worodria, Laurence Huang, Christina Yoon, J. Lucian Davis
  • Global Epidemiology and Evolutionary History of <span class="named-content genus-species" id="named-content-1">Staphylococcus aureus</span> ST45
    Epidemiology
    Global Epidemiology and Evolutionary History of Staphylococcus aureus ST45

    Staphylococcus aureus ST45 is a major global MRSA lineage with huge strain diversity and a high clinical impact. It is one of the most prevalent carrier lineages but also frequently causes severe invasive disease, such as bacteremia. Little is known about its evolutionary history. In this study, we used whole-genome sequencing to analyze a large collection of 451...

    N. Effelsberg, M. Stegger, L. Peitzmann, O. Altinok, G. W. Coombs, B. Pichon, A. Kearns, P. R. Randad, C. D. Heaney, S. Bletz, F. Schaumburg, A. Mellmann

Letters to the Editor

  • Free
    American Society for Microbiology Provides 2020 Guidelines for Detection and Identification of Group B <em>Streptococcus</em>
    Letter to the Editor
    American Society for Microbiology Provides 2020 Guidelines for Detection and Identification of Group B Streptococcus
    Laura Filkins, Jocelyn R. Hauser, Barbara Robinson-Dunn, Robert Tibbetts, Bobby L. Boyanton, Paula Revell
    and on behalf of the American Society for Microbiology Clinical and Public Health Microbiology Committee, Subcommittee on Laboratory Practices
  • Free
    Sensitive Recovery of Complete SARS-CoV-2 Genomes from Clinical Samples by Use of Swift Biosciences’ SARS-CoV-2 Multiplex Amplicon Sequencing Panel
    Letter to the Editor
    Sensitive Recovery of Complete SARS-CoV-2 Genomes from Clinical Samples by Use of Swift Biosciences’ SARS-CoV-2 Multiplex Amplicon Sequencing Panel
    Amin Addetia, Michelle J. Lin, Vikas Peddu, Pavitra Roychoudhury, Keith R. Jerome, Alexander L. Greninger
  • Free
    Combined Self-Collected Anterior Nasal and Oropharyngeal Specimens versus Provider-Collected Nasopharyngeal Swabs for the Detection of SARS-CoV-2
    Letter to the Editor
    Combined Self-Collected Anterior Nasal and Oropharyngeal Specimens versus Provider-Collected Nasopharyngeal Swabs for the Detection of SARS-CoV-2
    S. M. Shakir, A. P. Barker, D. R. Hillyard, N. Gilmore, J. W. Barrett, R. R. Orlandi, K. E. Hanson
  • MIC Ranges of Quality Control Strain <span class="named-content genus-species" id="named-content-1">Mycobacterium peregrinum</span> ATCC 700686 against Rifabutin, Eravacycline, Delafloxacin, Clofazimine, and Bedaquiline
    Letter to the Editor
    MIC Ranges of Quality Control Strain Mycobacterium peregrinum ATCC 700686 against Rifabutin, Eravacycline, Delafloxacin, Clofazimine, and Bedaquiline
    Matilda Boey, Zhehao Liu, Jeanette Teo, Sophie Octavia, Bintou A. Ahidjo, Ka Lip Chew
  • Open Access
    Identification of a Polymorphism in the N Gene of SARS-CoV-2 That Adversely Impacts Detection by Reverse Transcription-PCR
    Letter to the Editor
    Identification of a Polymorphism in the N Gene of SARS-CoV-2 That Adversely Impacts Detection by Reverse Transcription-PCR
    Manu Vanaerschot, Sabrina A. Mann, James T. Webber, Jack Kamm, Sidney M. Bell, John Bell, Si Noon Hong, Minh Phuong Nguyen, Lienna Y. Chan, Karan D. Bhatt, Michelle Tan, Angela M. Detweiler, Alex Espinosa, Wesley Wu, Joshua Batson, David Dynerman, Debra A. Wadford, Andreas S. Puschnik, Norma Neff, Vida Ahyong, Steve Miller, Patrick Ayscue, Cristina M. Tato, Simon Paul, Amy L. Kistler, Joseph L. DeRisi, Emily D. Crawford

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  • Laboratory Diagnosis of COVID-19: Current Issues and Challenges
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