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Journal of Clinical Microbiology
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Advanced Search

  • Home
  • Articles
    • Current Issue
    • Accepted Manuscripts
    • COVID-19 Special Collection
    • Archive
    • Minireviews
  • For Authors
    • Submit a Manuscript
    • Scope
    • Editorial Policy
    • Submission, Review, & Publication Processes
    • Organization and Format
    • Errata, Author Corrections, Retractions
    • Illustrations and Tables
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    • Abbreviations and Conventions
    • Publication Fees
    • Ethics Resources and Policies
  • About the Journal
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  • Rapid Test for Detection of Cefazolin Inoculum Effect in MSSA
    Rapid Test for Detection of Cefazolin Inoculum Effect in MSSA
  • Chromogenic Media for Detection of Candida auris
    Chromogenic Media for Detection of Candida auris
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  • Current Issue
  • Accepted Manuscripts
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Photo Quiz

  • Photo Quiz: Disseminated Violaceous Skin Lesions following Allogeneic Stem Cell Transplant
    Photo Quiz
    Photo Quiz: Disseminated Violaceous Skin Lesions following Allogeneic Stem Cell Transplant
    Hugh Adler, James Cruise, Jun Yong, Amit Patel, Magda Mikhail, Michael B. J. Beadsworth, Nicholas J. Beeching
  • Answer to March 2021 Photo Quiz
    Photo Quiz
    Answer to March 2021 Photo Quiz
    Hugh Adler, James Cruise, Jun Yong, Amit Patel, Magda Mikhail, Michael B. J. Beadsworth, Nicholas J. Beeching

The Brief Case

  • Free
    The Brief Case: A Maggot Mystery—<span class="named-content genus-species" id="named-content-1">Ignatzschineria larvae</span> Sepsis Secondary to an Infested Wound
    The Brief Case
    The Brief Case: A Maggot Mystery—Ignatzschineria larvae Sepsis Secondary to an Infested Wound
    Lanny T. DiFranza, Medini K. Annavajhala, Anne-Catrin Uhlemann, Daniel A. Green
  • Free
    Closing The Brief Case: A Maggot Mystery—<span class="named-content genus-species" id="named-content-1">Ignatzschineria larvae</span> Sepsis Secondary to an Infested Wound
    The Brief Case
    Closing The Brief Case: A Maggot Mystery—Ignatzschineria larvae Sepsis Secondary to an Infested Wound
    Lanny T. DiFranza, Medini K. Annavajhala, Anne-Catrin Uhlemann, Daniel A. Green

Commentary

  • Open Access
    Laboratory Automation in the Microbiology Laboratory: an Ongoing Journey, Not a Tale?
    Commentary
    Laboratory Automation in the Microbiology Laboratory: an Ongoing Journey, Not a Tale?

    Clinical chemistry laboratories implemented fully automated devices decades before microbiologists started their subtle approaches to follow. Meanwhile several papers have been published about reduced time to reports, faster workflows, and increased sensitivity as results of lab automation.

    Stefan Zimmermann

Minireviews

  • Open Access
    SHERLOCK and DETECTR: CRISPR-Cas Systems as Potential Rapid Diagnostic Tools for Emerging Infectious Diseases
    Minireview
    SHERLOCK and DETECTR: CRISPR-Cas Systems as Potential Rapid Diagnostic Tools for Emerging Infectious Diseases

    Infectious diseases are one of the most intimidating threats to human race, responsible for an immense burden of disabilities and deaths. Rapid diagnosis and treatment of infectious diseases offers a better understanding of their pathogenesis. According to the World Health Organization, the ideal approach for detecting foreign pathogens should be rapid, specific, sensitive, instrument-free, and cost-effective. Nucleic acid pathogen...

    Mujahed I. Mustafa, Abdelrafie M. Makhawi
  • Open Access
    Tuberculous Meningitis: Pathogenesis, Immune Responses, Diagnostic Challenges, and the Potential of Biomarker-Based Approaches
    Minireview
    Tuberculous Meningitis: Pathogenesis, Immune Responses, Diagnostic Challenges, and the Potential of Biomarker-Based Approaches

    Tuberculous meningitis (TBM) is the most devastating form of tuberculosis (TB), causing high mortality or disability. Clinical management of the disease is challenging due to limitations of the existing diagnostic approaches. Our knowledge on the immunology and pathogenesis of the disease is currently limited. More research is urgently needed to enhance our understanding of the immunopathogenesis of the disease and guide us toward the...

    Charles M. Manyelo, Regan S. Solomons, Gerhard Walzl, Novel N. Chegou
  • Potential Contributions of Anaerobes in Cystic Fibrosis Airways
    Minireview
    Potential Contributions of Anaerobes in Cystic Fibrosis Airways

    Cystic fibrosis (CF) is the most common, lethal genetic disease among the Caucasian population. The leading cause of mortality is recurrent acute exacerbations resulting in chronic airway inflammation and subsequent downward progression of pulmonary function. Traditionally, these periods of clinical deterioration have been associated with several principal pathogens. However, a growing body of literature has demonstrated a polymicrobial...

    Christina S. Thornton, Michael G. Surette

Bacteriology

  • Multicenter Evaluation of Ceftazidime-Avibactam Susceptibility Testing of <em>Enterobacterales</em> and <span class="named-content genus-species" id="named-content-1">Pseudomonas aeruginosa</span> on the Vitek 2 System
    Bacteriology
    Multicenter Evaluation of Ceftazidime-Avibactam Susceptibility Testing of Enterobacterales and Pseudomonas aeruginosa on the Vitek 2 System

    In this multisite study, Vitek 2 AST-Gram-Negative Ceftazidime/Avibactam test results for 1,073 isolates (866 Enterobacterales and 207 Pseudomonas aeruginosa) were compared to the Clinical and Laboratory Standards Institute (CLSI) broth microdilution (BMD) reference method. The results were analyzed for essential agreement (EA), category agreement (CA), major...

    Romney Humphries, Shelley Campeau, Thomas E. Davis, Kristin J. Nagaro, Vincent J. LaBombardi, Simone Franklin, Lisa Heimbach, Hari P. Dwivedi
  • Open Access
    Benefits Derived from Full Laboratory Automation in Microbiology: a Tale of Four Laboratories
    Bacteriology
    Benefits Derived from Full Laboratory Automation in Microbiology: a Tale of Four Laboratories

    Automation in clinical microbiology is starting to become more commonplace and reportedly offers several advantages over the manual laboratory. Most studies have reported on the rapid turnaround times for culture results, including times for identification of pathogens and their respective antimicrobial susceptibilities, but few have studied the benefits from a laboratory efficiency point of view.

    Karissa Culbreath, Heather Piwonka, John Korver, Mir Noorbakhsh
  • The Pediatric Endotracheal Aspirate Culture Survey (PETACS): Examining Practice Variation across Pediatric Microbiology Laboratories in the United States
    Bacteriology
    The Pediatric Endotracheal Aspirate Culture Survey (PETACS): Examining Practice Variation across Pediatric Microbiology Laboratories in the United States

    In the absence of evidence-based laboratory guidelines, the workup and interpretation of tracheal aspirate (TA) cultures remains controversial and confusing within the fields of clinical microbiology, infectious diseases, and critical care. Between 22 January and 24 February 2020, we conducted a national, web-based survey of microbiology laboratory personnel in free-standing pediatric hospitals and adult hospitals containing pediatric...

    Andrea M. Prinzi, Sarah K. Parker, Donna J. Curtis, Sonja I. Ziniel
  • Evaluation of Optimal Blood Culture Incubation Time To Maximize Clinically Relevant Results from a Contemporary Blood Culture Instrument and Media System
    Bacteriology
    Evaluation of Optimal Blood Culture Incubation Time To Maximize Clinically Relevant Results from a Contemporary Blood Culture Instrument and Media System

    Timely diagnosis of microorganisms in blood cultures is necessary to optimize therapy. Although blood culture media and systems have evolved for decades, the standard interval for incubation prior to being discarded as negative has remained 5 days. Here, we evaluated the optimal incubation time for the BacT/Alert Virtuo blood culture detection system (bioMérieux) using FA Plus (aerobic) and FN Plus (anaerobic) resin culture bottles in...

    Eric M. Ransom, Zahra Alipour, Meghan A. Wallace, Carey-Ann D. Burnham
  • Multicenter Evaluation of the Unyvero Platform for Testing Bronchoalveolar Lavage Fluid
    Bacteriology
    Multicenter Evaluation of the Unyvero Platform for Testing Bronchoalveolar Lavage Fluid

    Bronchoalveolar lavage (BAL) culture is a standard, though time-consuming, approach for identifying microorganisms in patients with severe lower respiratory tract infections. The sensitivity of BAL culture is relatively low, and prior antimicrobial therapy decreases the sensitivity further, leading to overuse of empirical antibiotics.

    Matthias Klein, Johannes Bacher, Sandra Barth, Faranak Atrzadeh, Katja Siebenhaller, Inês Ferreira, Stephan Beisken, Andreas E. Posch, Karen C. Carroll, Richard G. Wunderink, Chao Qi, Fann Wu, Dwight J. Hardy, Robin Patel, Matthew D. Sims
  • Open Access
    Assessing the Genetic Diversity of Austrian <span class="named-content genus-species" id="named-content-1">Corynebacterium diphtheriae</span> Clinical Isolates, 2011 to 2019
    Bacteriology
    Assessing the Genetic Diversity of Austrian Corynebacterium diphtheriae Clinical Isolates, 2011 to 2019

    Diphtheria is a vaccine-preventable disease with a high potential for reemergence. One of its causative agents is Corynebacterium diphtheriae, with some strains producing diphtheria toxin.

    Justine Schaeffer, Steliana Huhulescu, Anna Stoeger, Franz Allerberger, Werner Ruppitsch

Mycobacteriology and Aerobic Actinomycetes

  • Open Access
    Diagnostic Accuracy Study of a Novel Blood-Based Assay for Identification of Tuberculosis in People Living with HIV
    Mycobacteriology and Aerobic Actinomycetes
    Diagnostic Accuracy Study of a Novel Blood-Based Assay for Identification of Tuberculosis in People Living with HIV

    A nonsputum triage test to rule out tuberculosis (TB) disease is a WHO high-priority diagnostic, and a combinatory score based on a 3-gene host signature has shown promise in discriminating TB from other illnesses. We evaluated the accuracy of an early-prototype cartridge assay (“Xpert MTB Host Response” or Xpert-MTB-HR-Prototype) of this 3-gene signature on biobanked blood samples from people living with HIV (PLHIV) against a...

    Erik Södersten, Stefano Ongarello, Anna Mantsoki, Romain Wyss, David H. Persing, Sara Banderby, Linda Strömqvist Meuzelaar, Jacqueline Prieto, Devasena Gnanashanmugam, Purvesh Khatri, Samuel G. Schumacher, Claudia M. Denkinger
  • Open Access
    Comparative Analytical Evaluation of Four Centralized Platforms for the Detection of <span class="named-content genus-species" id="named-content-1">Mycobacterium tuberculosis</span> Complex and Resistance to Rifampicin and Isoniazid
    Mycobacteriology and Aerobic Actinomycetes
    Comparative Analytical Evaluation of Four Centralized Platforms for the Detection of Mycobacterium tuberculosis Complex and Resistance to Rifampicin and Isoniazid

    Failure to rapidly identify drug-resistant tuberculosis (TB) increases the risk of patient mismanagement, the amplification of drug resistance, and ongoing transmission. We generated comparative analytical data for four automated assays for the detection of TB and multidrug-resistant TB (MDR-TB): Abbott RealTime MTB and MTB RIF/INH (Abbott), Hain Lifescience FluoroType MTBDR (Hain), BD Max MDR-TB (BD), and Roche cobas MTB and...

    Margaretha de Vos, Lesley Scott, Anura David, Andre Trollip, Harald Hoffmann, Sophia Georghiou, Sergio Carmona, Morten Ruhwald, Wendy Stevens, Claudia M. Denkinger, Samuel G. Schumacher
  • Xpert MTB/XDR: a 10-Color Reflex Assay Suitable for Point-of-Care Settings To Detect Isoniazid, Fluoroquinolone, and Second-Line-Injectable-Drug Resistance Directly from <span class="named-content genus-species" id="named-content-1">Mycobacterium tuberculosis</span>-Positive Sputum
    Mycobacteriology and Aerobic Actinomycetes
    Xpert MTB/XDR: a 10-Color Reflex Assay Suitable for Point-of-Care Settings To Detect Isoniazid, Fluoroquinolone, and Second-Line-Injectable-Drug Resistance Directly from Mycobacterium tuberculosis-Positive Sputum

    We describe the design, development, analytical performance, and a limited clinical evaluation of the 10-color Xpert MTB/XDR assay (CE-IVD only, not for sale in the United States). This assay is intended as a reflex test to detect resistance to isoniazid (INH), fluoroquinolones (FLQ), ethionamide (ETH), and second-line injectable drugs (SLIDs) in unprocessed sputum samples and concentrated sputum sediments which are positive for...

    Yuan Cao, Heta Parmar, Rajiv L. Gaur, Deanna Lieu, Shobana Raghunath, Nova Via, Simone Battaglia, Daniela M. Cirillo, Claudia Denkinger, Sophia Georghiou, Robert Kwiatkowski, David Persing, David Alland, Soumitesh Chakravorty

Mycology

  • Development of a Duplex Real-Time PCR Assay for the Differentiation of <span class="named-content genus-species" id="named-content-1">Blastomyces dermatitidis</span> and <em>Blastomyces gilchristii</em> and a Retrospective Analysis of Culture and Primary Specimens from Blastomycosis Cases from New York (2005 to 2019)
    Mycology
    Development of a Duplex Real-Time PCR Assay for the Differentiation of Blastomyces dermatitidis and Blastomyces gilchristii and a Retrospective Analysis of Culture and Primary Specimens from Blastomycosis Cases from New York (2005 to 2019)

    Blastomycosis due to Blastomyces dermatitidis and Blastomyces gilchristii is a significant cause of respiratory mycoses in North America with occasional reported outbreaks. We developed a highly sensitive, specific, and reproducible TaqMan duplex real-time PCR assay for the differentiation of...

    Mitchell Kaplan, YanChun Zhu, Julianne V. Kus, Lisa McTaggart, Vishnu Chaturvedi, Sudha Chaturvedi
  • Free
    Frequency of Positive <em>Aspergillus</em> Tests in COVID-19 Patients in Comparison to Other Patients with Pulmonary Infections Admitted to the Intensive Care Unit
    Mycology
    Frequency of Positive Aspergillus Tests in COVID-19 Patients in Comparison to Other Patients with Pulmonary Infections Admitted to the Intensive Care Unit

    The aim of this study was to describe the frequency of positive Aspergillus tests in COVID-19 patients and investigate the association between COVID-19 and a positive Aspergillus test result. We compared the proportion of positive Aspergillus tests in COVID-19 patients admitted to the intensive care unit (ICU) for >24 h with two control groups: patients with community-acquired pneumonia with (i) a PCR-...

    Erlangga Yusuf, Alieke Vonk, Johannes P. C. van den Akker, Lonneke Bode, Gregorius J. Sips, Bart J. A. Rijnders, Jurriaan de Steenwinkel, Nelianne J. Verkaik, Marius Vogel, Menno van der Eerden, Mireille van Westreenen
  • A Short-Tandem-Repeat Assay (<em>Mmy</em>STR) for Studying Genetic Variation in <em>Madurella mycetomatis</em>
    Mycology
    A Short-Tandem-Repeat Assay (MmySTR) for Studying Genetic Variation in Madurella mycetomatis

    Madurella mycetomatis is the major causative agent of eumycetoma, a neglected tropical infection characterized by painless subcutaneous lesions, inflammation, and grains draining from multiple sinuses. To study the epidemiology of mycetoma, a robust discriminatory typing technique is needed.

    Bertrand Nyuykonge, Kimberly Eadie, Willemien H. A. Zandijk, Sarah A. Ahmed, Marie Desnos-Ollivier, Ahmed H. Fahal, Sybren de Hoog, Annelies Verbon, Wendy W. J. van de Sande, Corné H. W. Klaassen
  • Evaluation of the Dynamiker Cryptococcal Antigen Lateral Flow Assay for the Diagnosis of HIV-Associated Cryptococcosis
    Mycology
    Evaluation of the Dynamiker Cryptococcal Antigen Lateral Flow Assay for the Diagnosis of HIV-Associated Cryptococcosis

    Cryptococcal meningitis is a leading cause of meningitis in sub-Saharan Africa. Given the need for rapid point-of-care testing, we evaluated the diagnostic performance of the Dynamiker cryptococcal antigen (CrAg) lateral flow assay (LFA).

    Richard Kwizera, Denis Omali, Kiiza Tadeo, John Kasibante, Morris K. Rutakingirwa, Enock Kagimu, Kenneth Ssebambulidde, Darlisha A. Williams, Joshua Rhein, David Boulware, David B. Meya

Parasitology

  • Development of a Subtyping Tool for Zoonotic Pathogen <em>Cryptosporidium canis</em>
    Parasitology
    Development of a Subtyping Tool for Zoonotic Pathogen Cryptosporidium canis

    Cryptosporidium canis is an important cause of cryptosporidiosis in canines and humans. Studies of the transmission characteristics of C. canis are currently hampered by the lack of suitable subtyping tools.

    Wen Jiang, Dawn M. Roellig, Yaqiong Guo, Na Li, Yaoyu Feng, Lihua Xiao

Virology

  • Comparison of ZMAC and MARC-145 Cell Lines for Improving Porcine Reproductive and Respiratory Syndrome Virus Isolation from Clinical Samples
    Virology
    Comparison of ZMAC and MARC-145 Cell Lines for Improving Porcine Reproductive and Respiratory Syndrome Virus Isolation from Clinical Samples

    The MARC-145 cell line is commonly used to isolate porcine reproductive and respiratory syndrome virus (PRRSV) for diagnostics, research, and vaccine production, but it yields frustratingly low success rates of virus isolation (VI). The ZMAC cell line, derived from porcine alveolar macrophages, has become available, but its utilization for PRRSV VI from clinical samples has not been evaluated.

    Wannarat Yim-im, Haiyan Huang, Jie Park, Chong Wang, Gabriela Calzada, Phillip Gauger, Karen Harmon, Rodger Main, Jianqiang Zhang
  • Open Access
    Pooled Saliva Specimens for SARS-CoV-2 Testing
    Virology
    Pooled Saliva Specimens for SARS-CoV-2 Testing

    We evaluated saliva (SAL) specimens for SARS-CoV-2 reverse transcriptase PCR (RT-PCR) testing by comparison of 459 prospectively paired nasopharyngeal (NP) or midturbinate (MT) swabs from 449 individuals with the aim of using saliva for asymptomatic screening. Samples were collected in a drive-through car line for symptomatic individuals (n = 380) and in the emergency department (ED) (n = 69).

    Bidisha Barat, Sanchita Das, Valeria De Giorgi, David K. Henderson, Stacy Kopka, Anna F. Lau, Tracey Miller, Theresa Moriarty, Tara N. Palmore, Shari Sawney, Chris Spalding, Patricia Tanjutco, Glenn Wortmann, Adrian M. Zelazny, Karen M. Frank
  • Open Access
    Study To Evaluate the Performance of a Point-of-Care Whole-Blood HIV Viral Load Test (SAMBA II HIV-1 Semi-Q Whole Blood)
    Virology
    Study To Evaluate the Performance of a Point-of-Care Whole-Blood HIV Viral Load Test (SAMBA II HIV-1 Semi-Q Whole Blood)

    Remote areas of many low and middle income (LMI) countries have poor access to HIV viral load (HIV VL) testing. The SAMBA II (simple amplification-based assay) Semi-Q whole-blood test (Diagnostics for the Real World [DRW], Cambridge, UK) is a point-of-care assay, which uses leucodepletion technology to allow whole-blood testing in remote settings.

    Gary Brook, Tetiana Stepchenkova, Innocent M. Ali, Sandra Chipuka, Neha Goel, Helen Lee
  • Open Access
    Variable Sensitivity of SARS-CoV-2 Molecular Detection in European Expert Laboratories: External Quality Assessment, June and July 2020
    Virology
    Variable Sensitivity of SARS-CoV-2 Molecular Detection in European Expert Laboratories: External Quality Assessment, June and July 2020

    During the ongoing coronavirus disease 2019 (COVID-19) outbreak, robust detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a key element for clinical management and to interrupt transmission chains. We organized an external quality assessment (EQA) of molecular detection of SARS-CoV-2 for European expert laboratories. An EQA panel composed of 12 samples, containing either SARS-CoV-2 at different concentrations...

    Carlo Fischer, Ramona Mögling, Angeliki Melidou, Arne Kühne, Edmilson F. Oliveira-Filho, Thorsten Wolff, Janine Reiche, Eeva Broberg, Christian Drosten, Adam Meijer, Katrin Leitmeyer, Jan Felix Drexler, Chantal B. E. M. Reusken
  • Free
    Analytical Sensitivity of the Abbott BinaxNOW COVID-19 Ag Card
    Virology
    Analytical Sensitivity of the Abbott BinaxNOW COVID-19 Ag Card

    Multiple rapid antigen (Ag) tests for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have recently received emergency-use authorization (EUA) from the U.S. Food and Drug Administration (FDA).

    Garrett A. Perchetti, Meei-Li Huang, Margaret G. Mills, Keith R. Jerome, Alexander L. Greninger
  • Open Access
    Multicenter Evaluation of the Cepheid Xpert Xpress SARS-CoV-2/Flu/RSV Test
    Virology
    Multicenter Evaluation of the Cepheid Xpert Xpress SARS-CoV-2/Flu/RSV Test

    With the approach of respiratory virus season in the Northern Hemisphere, clinical microbiology and public health laboratories will need rapid diagnostic assays to distinguish severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) from influenza virus and respiratory syncytial virus (RSV) infections for diagnosis and surveillance. In this study, the clinical performance of the Xpert Xpress SARS-CoV-2/Flu/RSV test (Cepheid,...

    Heba H. Mostafa, Karen C. Carroll, Rachel Hicken, Gregory J. Berry, Ryhana Manji, Elizabeth Smith, Jennifer L. Rakeman, Randal C. Fowler, Mindy Leelawong, Susan M. Butler-Wu, David Quintero, Minette Umali-Wilcox, Robert W. Kwiatkowski, David H. Persing, Fred Weir, Michael J. Loeffelholz

Immunoassays

  • Free
    Multicenter Evaluation of the Clinical Performance and the Neutralizing Antibody Activity Prediction Properties of 10 High-Throughput Serological Assays Used in Clinical Laboratories
    Immunoassays
    Multicenter Evaluation of the Clinical Performance and the Neutralizing Antibody Activity Prediction Properties of 10 High-Throughput Serological Assays Used in Clinical Laboratories

    As the coronavirus disease 2019 (COVID-19) pandemic second wave is emerging, it is of the upmost importance to screen the population immunity in order to keep track of infected individuals. Consequently, immunoassays for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) with high specificity and positive predictive values are needed to obtain an accurate epidemiological picture.

    C. Therrien, B. Serhir, M. Bélanger-Collard, J. Skrzypczak, D. K. Shank, C. Renaud, J. Girouard, V. Loungnarath, M. Carrier, G. Brochu, F. Tourangeau, B. Gilfix, A. Piche, R. Bazin, R. Guérin, M. Lavoie, V. Martel-Laferrière, C. Fortin, A. Benoit, D. Marcoux, N. Gauthier, A. M. Laumaea, R. Gasser, A. Finzi, M. Roger

Clinical Veterinary Microbiology

  • Development of an Indirect Chemiluminescence Immunoassay Using a Multiepitope Recombinant Protein To Specifically Detect Antibodies against Foot-and-Mouth Disease Virus Serotype O in Swine
    Clinical Veterinary Microbiology
    Development of an Indirect Chemiluminescence Immunoassay Using a Multiepitope Recombinant Protein To Specifically Detect Antibodies against Foot-and-Mouth Disease Virus Serotype O in Swine

    Foot-and-mouth disease virus (FMDV) has led to serious losses in animal husbandry worldwide. Seromonitoring of FMDV postvaccination is important for the control and eradication of foot-and-mouth disease (FMD) in regions and countries where vaccination is widespread. However, many commercial kits present high false-positive rates.

    Wei Liu, Junjun Shao, Guanglei Zhang, Yanyan Chang, Sudan Ge, Yue Sun, Zhan Gao, Huiyun Chang
  • Distribution of Novel Og Types in Shiga Toxin-Producing <span class="named-content genus-species" id="named-content-1">Escherichia coli</span> Isolated from Healthy Cattle
    Clinical Veterinary Microbiology
    Distribution of Novel Og Types in Shiga Toxin-Producing Escherichia coli Isolated from Healthy Cattle

    Shiga toxin-producing Escherichia coli (STEC) is an important foodborne pathogen. Although most cases of STEC infection in humans are due to O157 and non-O157 serogroups, there are also reports of infection with STEC strains that cannot be serologically classified into any O serogroup (O-serogroup untypeable [OUT]).

    Thi Thu Huong Nguyen, Atsushi Iguchi, Ritsuko Ohata, Hisahiro Kawai, Tadasuke Ooka, Hiroshi Nakajima, Sunao Iyoda

Epidemiology

  • Establishment and Evaluation of a Core Genome Multilocus Sequence Typing Scheme for Whole-Genome Sequence-Based Typing of <span class="named-content genus-species" id="named-content-1">Pseudomonas aeruginosa</span>
    Epidemiology
    Establishment and Evaluation of a Core Genome Multilocus Sequence Typing Scheme for Whole-Genome Sequence-Based Typing of Pseudomonas aeruginosa

    The environmental bacterium Pseudomonas aeruginosa, particularly multidrug-resistant clones, is often associated with nosocomial infections and outbreaks. Today, core genome multilocus sequence typing (cgMLST) is frequently applied to delineate sporadic cases from nosocomial transmissions.

    Hauke Tönnies, Karola Prior, Dag Harmsen, Alexander Mellmann
  • Cross-Border Emergence of <span class="named-content genus-species" id="named-content-1">Escherichia coli</span> Producing the Carbapenemase NDM-5 in Switzerland and Germany
    Epidemiology
    Cross-Border Emergence of Escherichia coli Producing the Carbapenemase NDM-5 in Switzerland and Germany

    A series of clinical NDM-5-producing Escherichia coli isolates obtained from two surveillance networks for carbapenem-producing Enterobacterales from 2018 to 2019, namely, Switzerland (NARA) and Germany (SurvCARE), were analyzed. The 33 NDM-5-producing E. coli isolates were highly resistant...

    Trinad Chakraborty, Mustafa Sadek, Yancheng Yao, Can Imirzalioglu, Roger Stephan, Laurent Poirel, Patrice Nordmann
  • Molecular and Epidemiological Characterization of Toxigenic and Nontoxigenic <span class="named-content genus-species" id="named-content-1">Corynebacterium diphtheriae</span>, <span class="named-content genus-species" id="named-content-2">Corynebacterium belfantii</span>, <span class="named-content genus-species" id="named-content-3">Corynebacterium rouxii</span>, and <span class="named-content genus-species" id="named-content-4">Corynebacterium ulcerans</span> Isolates Identified in Spain from 2014 to 2019
    Epidemiology
    Molecular and Epidemiological Characterization of Toxigenic and Nontoxigenic Corynebacterium diphtheriae, Corynebacterium belfantii, Corynebacterium rouxii, and Corynebacterium ulcerans Isolates Identified in Spain from 2014 to 2019

    This study examines the microbiological and epidemiological characteristics of toxigenic and nontoxigenic Corynebacterium isolates submitted to the national reference laboratory in Spain, between 2014 and 2019, in order to describe the current situation and improve our knowledge regarding these emerging pathogens. Epidemiological information was extracted from the Spanish Surveillance System.

    Andreas Hoefer, Despina Pampaka, Silvia Herrera-León, Sonia Peiró, Sarai Varona, Noemí López-Perea, Josefa Masa-Calles, Laura Herrera-León
  • High-Resolution Typing of <em>Staphylococcus epidermidis</em> Based on Core Genome Multilocus Sequence Typing To Investigate the Hospital Spread of Multidrug-Resistant Clones
    Epidemiology
    High-Resolution Typing of Staphylococcus epidermidis Based on Core Genome Multilocus Sequence Typing To Investigate the Hospital Spread of Multidrug-Resistant Clones

    Staphylococcus epidermidis is a pathogen emerging worldwide as a leading cause of health care-associated infections. A standardized high-resolution typing method to document transmission and dissemination of multidrug-resistant S. epidermidis strains is needed.

    Anne Jamet, Julien Guglielmini, Bryan Brancotte, Mathieu Coureuil, Daniel Euphrasie, Julie Meyer, Johanna Roux, Jean-Philippe Barnier, Emmanuelle Bille, Agnès Ferroni, Jean-François Magny, Christine Bôle-Feysot, Alain Charbit, Xavier Nassif, Sylvain Brisse

Letters to the Editor

  • New Names for Fungi of Medical Importance: Can We Have Our Cake and Eat It Too?
    Letter to the Editor
    New Names for Fungi of Medical Importance: Can We Have Our Cake and Eat It Too?
    Sarah E. Kidd, Catriona L. Halliday, Brendan McMullan, Sharon C.-A. Chen, Juliet Elvy
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  • When To Retest: an Examination of Repeat COVID-19 PCR Patterns in an Ambulatory Population
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