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Journal of Clinical Microbiology
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Most read article(s)

  • Open Access
    Laboratory Diagnosis of COVID-19: Current Issues and Challenges
    Commentary
    Laboratory Diagnosis of COVID-19: Current Issues and Challenges

    The COVID-19 outbreak has had a major impact on clinical microbiology laboratories in the past several months. This commentary covers current issues and challenges for the laboratory diagnosis of infections caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). In the preanalytical stage, collecting the proper respiratory tract specimen at the right time from the right anatomic site is essential for a prompt and...

    Yi-Wei Tang, Jonathan E. Schmitz, David H. Persing, Charles W. Stratton
  • Free
    When To Retest: an Examination of Repeat COVID-19 PCR Patterns in an Ambulatory Population
    Letter to the Editor
    When To Retest: an Examination of Repeat COVID-19 PCR Patterns in an Ambulatory Population
    Dina N. Greene, Jane A. Dickerson, Alexander L. Greninger, Robert L. Schmidt
  • Free
    Performance of Abbott ID Now COVID-19 Rapid Nucleic Acid Amplification Test Using Nasopharyngeal Swabs Transported in Viral Transport Media and Dry Nasal Swabs in a New York City Academic Institution
    Special Issue Virology
    Performance of Abbott ID Now COVID-19 Rapid Nucleic Acid Amplification Test Using Nasopharyngeal Swabs Transported in Viral Transport Media and Dry Nasal Swabs in a New York City Academic Institution

    The recent emergence of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has posed formidable challenges for clinical laboratories seeking reliable laboratory diagnostic confirmation. The swift advance of the crisis in the United States has led to Emergency Use Authorization (EUA) facilitating the availability of molecular diagnostic assays without the more rigorous examination to which tests are normally...

    Atreyee Basu, Tatyana Zinger, Kenneth Inglima, Kar-mun Woo, Onome Atie, Lauren Yurasits, Benjamin See, Maria E. Aguero-Rosenfeld
  • Free
    Quantitative Measurement of Anti-SARS-CoV-2 Antibodies: Analytical and Clinical Evaluation
    Immunoassays
    Quantitative Measurement of Anti-SARS-CoV-2 Antibodies: Analytical and Clinical Evaluation

    The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of coronavirus disease 2019 (COVID-19). While molecular-based testing is used to diagnose COVID-19, serologic testing of antibodies specific to SARS-CoV-2 is used to detect past infection.

    Victoria Higgins, Anselmo Fabros, Vathany Kulasingam
  • Improved Molecular Diagnosis of COVID-19 by the Novel, Highly Sensitive and Specific COVID-19-RdRp/Hel Real-Time Reverse Transcription-PCR Assay Validated <em>In Vitro</em> and with Clinical Specimens
    Virology
    Improved Molecular Diagnosis of COVID-19 by the Novel, Highly Sensitive and Specific COVID-19-RdRp/Hel Real-Time Reverse Transcription-PCR Assay Validated In Vitro and with Clinical Specimens

    On 31 December 2019, the World Health Organization was informed of a cluster of cases of pneumonia of unknown etiology in Wuhan, China. Subsequent investigations identified a novel coronavirus, now named severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), from the affected patients. Highly sensitive and specific laboratory diagnostics are important for controlling the rapidly evolving SARS-CoV-2-associated coronavirus disease...

    Jasper Fuk-Woo Chan, Cyril Chik-Yan Yip, Kelvin Kai-Wang To, Tommy Hing-Cheung Tang, Sally Cheuk-Ying Wong, Kit-Hang Leung, Agnes Yim-Fong Fung, Anthony Chin-Ki Ng, Zijiao Zou, Hoi-Wah Tsoi, Garnet Kwan-Yue Choi, Anthony Raymond Tam, Vincent Chi-Chung Cheng, Kwok-Hung Chan, Owen Tak-Yin Tsang, Kwok-Yung Yuen
  • Open Access
    Performance Characteristics of the Abbott Architect SARS-CoV-2 IgG Assay and Seroprevalence in Boise, Idaho
    Special Issue Immunoassays
    Performance Characteristics of the Abbott Architect SARS-CoV-2 IgG Assay and Seroprevalence in Boise, Idaho

    Coronavirus disease 2019 (COVID-19), the novel respiratory illness caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is associated with severe morbidity and mortality. The rollout of diagnostic testing in the United States was slow, leading to numerous cases that were not tested for SARS-CoV-2 in February and March 2020 and necessitating the use of serological testing to determine past infections. Here, we...

    Andrew Bryan, Gregory Pepper, Mark H. Wener, Susan L. Fink, Chihiro Morishima, Anu Chaudhary, Keith R. Jerome, Patrick C. Mathias, Alexander L. Greninger
  • Free
    Challenges and Controversies to Testing for COVID-19
    Commentary
    Challenges and Controversies to Testing for COVID-19

    The coronavirus disease (COVID-19) pandemic has placed the clinical laboratory and testing for SARS-CoV-2 front and center in the worldwide discussion of how to end the outbreak. Clinical laboratories have responded by developing, validating, and implementing a variety of molecular and serologic assays to test for SARS-CoV-2 infection. This has played an essential role in identifying cases, informing isolation decisions, and helping to...

    Matthew J. Binnicker
  • Free
    Evaluation of Nucleocapsid and Spike Protein-Based Enzyme-Linked Immunosorbent Assays for Detecting Antibodies against SARS-CoV-2
    Immunoassays
    Evaluation of Nucleocapsid and Spike Protein-Based Enzyme-Linked Immunosorbent Assays for Detecting Antibodies against SARS-CoV-2

    At present, PCR-based nucleic acid detection cannot meet the demands for coronavirus infectious disease (COVID-19) diagnosis. Two hundred fourteen confirmed COVID-19 patients who were hospitalized in the General Hospital of Central Theater Command of the People’s Liberation Army between 18 January and 26 February 2020 were recruited. Two enzyme-linked immunosorbent assay (ELISA) kits based on recombinant severe acute respiratory...

    Wanbing Liu, Lei Liu, Guomei Kou, Yaqiong Zheng, Yinjuan Ding, Wenxu Ni, Qiongshu Wang, Li Tan, Wanlei Wu, Shi Tang, Zhou Xiong, Shangen Zheng
  • Open Access
    Culture-Based Virus Isolation To Evaluate Potential Infectivity of Clinical Specimens Tested for COVID-19
    Special Issue Virology
    Culture-Based Virus Isolation To Evaluate Potential Infectivity of Clinical Specimens Tested for COVID-19

    Real-time reverse transcription-PCR (RT-PCR) is currently the most sensitive method to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) that causes coronavirus disease 2019 (COVID-19). However, the correlation between detectable viral RNA and culturable virus in clinical specimens remains unclear. Here, we performed virus culture for 60 specimens that were confirmed to be positive for SARS-CoV-2 RNA by real-time RT-...

    Chung-Guei Huang, Kuo-Ming Lee, Mei-Jen Hsiao, Shu-Li Yang, Peng-Nien Huang, Yu-Nong Gong, Tzu-Hsuan Hsieh, Po-Wei Huang, Ya-Jhu Lin, Yi-Chun Liu, Kuo-Chien Tsao, Shin-Ru Shih
  • Open Access
    The Role of Antibody Testing for SARS-CoV-2: Is There One?
    Special Issue Commentary
    The Role of Antibody Testing for SARS-CoV-2: Is There One?

    The emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) brought with it rapid development of both molecular and serologic assays for identification of COVID-19 infections. While Food and Drug Administration (FDA) emergency use authorization (EUA) is required for clinical application of SARS-CoV-2 molecular tests, submission for EUA is currently a voluntary process for manufacturers of serologic assays. The absence...

    Elitza S. Theel, Patricia Slev, Sarah Wheeler, Marc Roger Couturier, Susan J. Wong, Kamran Kadkhoda

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