DOI: 10.1128/JCM.36.1.90-93.1998
Article Figures & Data
Tables
- Table 1.
Clinical details
Sample Year of acquisition Age of patient (yr) Underlying immunodeficiency LT68 1968 Unknown Unknown US3846 1970 8a Unknown (low cellular and humoral responses) US3885 1970 25 Hypogammaglobulinemia US4001 1971 7 Stem-cell leukemia US4015 1971 25 None (bronchiectasis) SP81 1981 31 Chronic myeloid leukemia ↵a Eight months.
- Table 2.
Details of DNA amplification
Sample Type of PCR for gene encoding: mt LSU rRNA ITS arom locus mt SSU rRNA LT68 Single Single Nested Single US3846 Nested Nested Nested Nested US3885 Nested Nested Nested Nested US4001 Nested Nested US4015 Nested Nested SP81 Single Single - Table 3.
Oligonucleotides used
Primer Sequence pAZ102-H/RI 5′-GGGAATTCGTGTACGTTGCAAAGTACTC-3′ pAZ102-E/RI 5′-GGGAATTCGATGGCTGTTTCCAAGCCCA-3′ pAZ102-X/RI 5′-GGGAATTCGTGAAATACAAATCGGACTAGG-3′ pAZ102-Y/RI 5′-GGGAATTCTCACTTAATATTAATTGGGGAGC-3′ NITSF 5′-GTCGTAACAAGGTTTCCGTA-3′ NITSR 5′-CCTCCGCTTATTGATATGCT-3′ ITSF3/RI 5′-GGGAATTCCTGCGGAAGGATCATTAGAAA-3′ ITSF3/RI 5′-GGGAATTCGATTTGAGATTAAAATTCTTG-3′ pAZ112-10F/RI 5′-GGGAATTCTAGACGGTCACAGAGATCAG-3′ pAZ112-10R/RI 5′-GGGAATTCGAACGATTACTAGCAATTCC-3′ pAZ112-13/RI 5′-GGGAATTCGAAGCATGTTGTTTAATTCG-3′ pAZ112-14/RI 5′-GGGAATTCTTCAAAGAA(T,C)CGAGTT(T,C)CAG-3′ AroFunivB 5′-ATCCCACCAN(T,C)(A,C)NGGCCA-3′ AroFunivC 5′-(A,G)ATATGGA(A,G)(T,C)CAATGACNGA-3′ HsPcaro1/RI 5′-GGGAATTCTTAACAACAACAATTTTAGCC-3′ HsPcaro2/RI 5′-GGGAATTCGGTGAAATGCATGCCAAGAC-3′ - Table 4.
Nucleotide sequence types in P. carinii f. sp. hominisa
Sample ITS sequence type Nucleotide at indicated position in gene encoding: mt LSU rRNA mt SSU rRNA arom locus 81 85 248 160 196 121 208 LT68 B1a1 C T C C T C A B2a1 US3846 B1a1 C T NDd A G C A B2a1 C C ND C T B2a4 b US3885 A2c1 C T ND A G C A A2c2 b C C ND A3c1 b US4001 B1a3 C T ND ND ND ND ND B3a3 C C ND US4015 B1b2 C C ND ND ND ND ND SP81 B2a1 Tc C C ND ND ND ND ↵a New sequences were compared to sequences for ITS sequence types and the genes encoding mt LSU rRNA, mt SSU rRNA, and the arom locus from references 15, 14, 4, and 1, respectively, used as standards.
↵b New sequence type identified in this study.
↵c New variation at this nucleotide position.
↵d ND, not determined.