Article Figures & Data
Tables
- Table 1.
Prenatal diagnosis carried out by PCR and antigenemia testing with FB and by PCR and rapid virus isolation with AF in relation to outcome for fetus or newborn for pregnant women at risk of transmitting a CMV infection and control populationa
Group FB AF Total no. of specimens No. of specimens positive by PCR No. of specimens positive by AG testing Infected fetus or newborn Total no. of specimens No. of specimens positive by PCR No. of specimens positive by SV Infected fetus or newborn Fetus/newbornb Total no. Fetus/newborn Total no. Seroconversion for CMV IgG 10 2 1 0/1 1 16 9 1 1/3 4 IgM positivity by ELISA 39 10 1 2/3 5 61 14 4 3/3 6 Clinical symptoms (mother/fetus) 1 0 0 0 0 5 4 1 1/1 2 Total 50 12 2 2/4 6 82 25 5 5/7 12 Uninfected (EIA IgM negative) 11 0 0 0 0 20 0 0 0 0 - Table 2.
Sensitivity, specificity, PPV, and NPV of antigenemia tests and PCR with PMNLs from FB and of PCR and rapid virus isolation from the AF with regard to the detection of a CMV-infected fetus or newborn
Material Test Result No. of fetuses or newborns Sensitivitya Specificityb PPVc NPVd Infected Uninfected FB PCR Positive 4 8 66.6 85.5 33.3 96 Negative 2 47 Test for antigenemia Positive 1 1 16 98.2 50 91.5 Negative 5 54 AF PCR Positive 12 15 100 83.3 48 100 Negative 0 75 Shell vial assay Positive 6 0 50 100 100 93.8 Negative 6 90 ↵a (Number of true positives/total number of fetuses or newborns infected) × 100.
↵b (Number of true negatives/total number of fetuses or newborns uninfected) × 100.
↵c (Number of true positives/number of true positives + number of false positives) × 100.
↵d (Number of true negatives/number of false negatives + number of true negatives) × 100.
- Table 3.
Prenatal diagnosis and congenital infections in pregnant women with CMV-specific IgM as determined by EIA and WB
Group No. of women IgM-positive sera by EIA IgM-positive sera by WB by type of infection WB positive (n = 45) WB negative (n = 16) Primary (n = 21) Secondary (n = 8) NDa (n = 16) Prenatal diagnosis with AF PCR positive 14 0 11 1 2 Virus isolation positive 4 0 4 0 0 Congenital infections 6 0 6 0 0 ↵a ND, not determined.
- Table 4.
Sensitivity, specificity, PPV, and NPV of maternal serology with regard to the detection of a CMV-infected fetus or newborn
Test Result No. of fetuses or newborns Sensitivitya Specificityb PPVc NPVd Infected Uninfected Seroconversion Positive 4 12 100 62.5 25 100 Negative 0 20 IgM by ELISA Positive 6 55 100 26.6 9.8 100 Negative 0 20 IgM WB profile Positive 6 15 100 72.7 28.5 100 Negative 0 40 ↵a (Number of true positives/total number of fetuses or newborns infected) × 100.
↵b (Number of true negatives/total number of fetuses or newborns uninfected) × 100.
↵c (Number of true positives/number of true positives + number of false positives) × 100.
↵d (Number of true negatives/number of false negatives + number of true negatives) × 100.
