Article Figures & Data
Figures
- Fig. 1.
Polyacrylamide gel electrophoresis and silver staining showing gel marker (GelMarker I; Research Genetics, Huntsville, Ala.) (lane 1) and PCR products of S. pneumoniae ATCC 6301 (lane 2), S. pneumoniae ATCC 6314 (lane 3), S. pneumoniae ATCC 10341 (lane 4), S. pneumoniae ATCC 10341 (lane 6), S. pneumoniae ATCC 33400 (lane 7), S. mutans (lane 8), S. agalactiae (lane 9), S. pyogenes (lane 11), S. equi (lane 12), E. faecalis (lane 13), and reagent blanks (lanes 5, 10 and 14).
- Fig. 2.
Quantitative sensitivity of the PCR-EIA showing fluorescence values by EIA produced by the PCR products of serial dilutions of S. pneumoniae ATCC 6301 DNA and of other bacteria.
Tables
- Table 1.
Results of PCR-EIA for detection of S. pneumoniae with CSF specimens from patients with meningitis
CSF specimen source No. of specimens tested No. of specimens positive by PCR-EIA Patients with meningitis Culture or LA positive for S. pneumoniae 8 7 Culture or LA positive for H. influenzae 4 0 Culture positive for other organismsa 6 0 Culture and LA negative 11 5 Patients without meningitisb 9 1 ↵a Other includes Proteus mirabilis (n = 1), pseudomonas (n = 1), coagulase-negative staphylococci (n = 1), alpha-hemolytic streptococci (n = 1), and micrococci (n = 2).
↵b Febrile seizures (n = 2), congenital hydrocephalus (n = 1), neonatal sepsis without meningitis (n = 2), cytophagic panniculitis (n = 1), typhoid fever with encephalopathy (n = 1), pneumococcal pneumonia (n = 1), and pneumococcal scalp abscess (n = 1).
- Table 2.
Selected characteristics of patients with meningitis for whom culture and LA were negative but PCR-EIA was positivea
Patient no. Age Duration of illness (days) Prior treatment Mean OD value OD cutoff CSF findings Treatment and clinical course 1 5 yr 7 Unknown parenteral medication 0.258 0.038 WBC, 18/mm3; poly, 20%, glucose: 57 mg/dl; protein: 22 mg/dl i.v. chloramphenicol and penicillin; recovered 2 5 mo 14 Oral amoxycillin 0.804 0.038 WBC, 110/mm3; poly, 5%; glucose, 25 mg/dl; protein, 118 mg/dl Also had pneumonia, subdural effusion, and hydrocephalus; i.v. cefotaxime; LAMA 3 3 mo 4 Not known 0.440 0.034 WBC, 15/mm3; poly, 47%; glucose, 60 mg/dl; protein, 16 mg/dl i.v. cefotaxime; recovered 4 22 days 3 i.v. cefotaxime 0.212 0.025 WBC, 48/mm3; poly, 2%; glucose, 37 mg/dl; protein, 48 mg/dl Also had pneumonia; i.v. cefotaxime; died 5 7 yr 5 i.v. chloramphenicol 0.160 0.032 WBC, 120/mm3; poly, 30%; glucose, 63 mg/dl; protein, 22 mg/dl i.v. cefotaxime; recovered 0.174 0.027 ↵a Abbreviations: WBC, leukocyte count; Poly, polymorphonuclear leukocytes; i.v., intravenous; LAMA, left against medical advice.
