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Chlamydiology and Rickettsiology

Detection of Chlamydia trachomatis by the Gen-Probe AMPLIFIED Chlamydia Trachomatis Assay (AMP CT) in Urine Specimens from Men and Women and Endocervical Specimens from Women

Kimberly A. Crotchfelt, Barbara Pare, Charlotte Gaydos, Thomas C. Quinn
Kimberly A. Crotchfelt
Division of Infectious Diseases, Johns Hopkins University, Baltimore, Maryland,and
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Barbara Pare
Division of Infectious Diseases, Johns Hopkins University, Baltimore, Maryland,and
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Charlotte Gaydos
Division of Infectious Diseases, Johns Hopkins University, Baltimore, Maryland,and
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Thomas C. Quinn
Division of Infectious Diseases, Johns Hopkins University, Baltimore, Maryland,and
National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland
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DOI: 10.1128/JCM.36.2.391-394.1998
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Tables

  • Table 1.

    Analysis of AMP CT performance with specimens from males and females before and after discrepant analysis

    Gender, test, and resultNo. of samples with the following result:
    Before discrepant analysisAfter discrepant analysisa
    Culture positiveCulture negativeResolved positiveResolved negative
    Female
     AMP CT of endocervical swab specimens (n = 479)
      Positive3928652
      Negative04120412
     AMP CT of urine specimens (n = 480)
      Positive3426600
      Negative44164416
     AMP CT of matched endocervical swab and urine specimens (n = 474)
      Positive3533671
      Negative34033403
    Male, AMP CT of urine specimens (n = 464)
     Positive3733655
     Negative33913391
    • ↵a Discrepant analysis was performed by DFA staining of sedimented culture transport medium and by TMA with 16S rRNA as a target.

  • Table 2.

    Resolved performance characteristics for detectionC. trachomatis by AMP CT for gender and specimen type

    Gender and testSample typeNo. of samplesResolved performancea
    Sens. (%)Spec. (%)PPV (%)NPV (%)
    Female
     AMP CTEndocervical swab479100.099.597.0100.0
     AMP CTUrine48093.8100.0100.099.0
     AMP CTMatched urine and endocervical swab47495.799.898.599.3
     CultureEndocervical swab47454.3100.0100.092.7
    Male
     AMP CTUrine46495.698.792.999.2
     CultureUrethral swab46458.8100.0100.093.4
    • ↵a Sens., sensitivity; Spec., specificity; PPV, positive predictive value; NPV, negative predictive value.

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Detection of Chlamydia trachomatis by the Gen-Probe AMPLIFIED Chlamydia Trachomatis Assay (AMP CT) in Urine Specimens from Men and Women and Endocervical Specimens from Women
Kimberly A. Crotchfelt, Barbara Pare, Charlotte Gaydos, Thomas C. Quinn
Journal of Clinical Microbiology Feb 1998, 36 (2) 391-394; DOI: 10.1128/JCM.36.2.391-394.1998

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Detection of Chlamydia trachomatis by the Gen-Probe AMPLIFIED Chlamydia Trachomatis Assay (AMP CT) in Urine Specimens from Men and Women and Endocervical Specimens from Women
Kimberly A. Crotchfelt, Barbara Pare, Charlotte Gaydos, Thomas C. Quinn
Journal of Clinical Microbiology Feb 1998, 36 (2) 391-394; DOI: 10.1128/JCM.36.2.391-394.1998
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KEYWORDS

Cervix Uteri
Chlamydia Infections
Chlamydia trachomatis
DNA, Bacterial
RNA, Ribosomal, 23S

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