Skip to main content
  • ASM
    • Antimicrobial Agents and Chemotherapy
    • Applied and Environmental Microbiology
    • Clinical Microbiology Reviews
    • Clinical and Vaccine Immunology
    • EcoSal Plus
    • Eukaryotic Cell
    • Infection and Immunity
    • Journal of Bacteriology
    • Journal of Clinical Microbiology
    • Journal of Microbiology & Biology Education
    • Journal of Virology
    • mBio
    • Microbiology and Molecular Biology Reviews
    • Microbiology Resource Announcements
    • Microbiology Spectrum
    • Molecular and Cellular Biology
    • mSphere
    • mSystems
  • Log in
  • My alerts
  • My Cart

Main menu

  • Home
  • Articles
    • Current Issue
    • Accepted Manuscripts
    • COVID-19 Special Collection
    • Archive
    • Minireviews
  • For Authors
    • Submit a Manuscript
    • Scope
    • Editorial Policy
    • Submission, Review, & Publication Processes
    • Organization and Format
    • Errata, Author Corrections, Retractions
    • Illustrations and Tables
    • Nomenclature
    • Abbreviations and Conventions
    • Publication Fees
    • Ethics Resources and Policies
  • About the Journal
    • About JCM
    • Editor in Chief
    • Editorial Board
    • For Reviewers
    • For the Media
    • For Librarians
    • For Advertisers
    • Alerts
    • RSS
    • FAQ
  • Subscribe
    • Members
    • Institutions
  • ASM
    • Antimicrobial Agents and Chemotherapy
    • Applied and Environmental Microbiology
    • Clinical Microbiology Reviews
    • Clinical and Vaccine Immunology
    • EcoSal Plus
    • Eukaryotic Cell
    • Infection and Immunity
    • Journal of Bacteriology
    • Journal of Clinical Microbiology
    • Journal of Microbiology & Biology Education
    • Journal of Virology
    • mBio
    • Microbiology and Molecular Biology Reviews
    • Microbiology Resource Announcements
    • Microbiology Spectrum
    • Molecular and Cellular Biology
    • mSphere
    • mSystems

User menu

  • Log in
  • My alerts
  • My Cart

Search

  • Advanced search
Journal of Clinical Microbiology
publisher-logosite-logo

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Accepted Manuscripts
    • COVID-19 Special Collection
    • Archive
    • Minireviews
  • For Authors
    • Submit a Manuscript
    • Scope
    • Editorial Policy
    • Submission, Review, & Publication Processes
    • Organization and Format
    • Errata, Author Corrections, Retractions
    • Illustrations and Tables
    • Nomenclature
    • Abbreviations and Conventions
    • Publication Fees
    • Ethics Resources and Policies
  • About the Journal
    • About JCM
    • Editor in Chief
    • Editorial Board
    • For Reviewers
    • For the Media
    • For Librarians
    • For Advertisers
    • Alerts
    • RSS
    • FAQ
  • Subscribe
    • Members
    • Institutions
Mycobacteriology and Aerobic Actinomycetes

Comparative Evaluation of Initial and New Versions of the Gen-Probe Amplified Mycobacterium Tuberculosis Direct Test for Direct Detection of Mycobacterium tuberculosis in Respiratory and Nonrespiratory Specimens

Fredy Gamboa, Gregorio Fernandez, Eduardo Padilla, José M. Manterola, Joan Lonca, Pere Joan Cardona, Lurdes Matas, Vicente Ausina
Fredy Gamboa
Servicio de Microbiologia, Hospital Universitario Germans Trias i Pujol, and
Departamento de Microbiologı́a, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá, Colombia
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Gregorio Fernandez
Servicio de Microbiologia, Hospital Universitario Germans Trias i Pujol, and
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Eduardo Padilla
Servicio de Microbiologia, Hospital Universitario Germans Trias i Pujol, and
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
José M. Manterola
Servicio de Microbiologia, Hospital Universitario Germans Trias i Pujol, and
Departamento de Genética y Microbiologı́a, Facultad de Medicina, Universidad Autónoma de Barcelona, Barcelona, Spain, and
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Joan Lonca
Servicio de Microbiologia, Hospital Universitario Germans Trias i Pujol, and
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Pere Joan Cardona
Servicio de Microbiologia, Hospital Universitario Germans Trias i Pujol, and
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Lurdes Matas
Servicio de Microbiologia, Hospital Universitario Germans Trias i Pujol, and
Departamento de Genética y Microbiologı́a, Facultad de Medicina, Universidad Autónoma de Barcelona, Barcelona, Spain, and
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Vicente Ausina
Servicio de Microbiologia, Hospital Universitario Germans Trias i Pujol, and
Departamento de Genética y Microbiologı́a, Facultad de Medicina, Universidad Autónoma de Barcelona, Barcelona, Spain, and
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
DOI: 10.1128/JCM.36.3.684-689.1998
  • Article
  • Figures & Data
  • Info & Metrics
  • PDF
Loading

Article Figures & Data

Tables

  • Table 1.

    Detection of M. tuberculosis complex in respiratory specimens by initial and new versions of AMTDT

    Microscopy resultTest and resultNo. of specimens with the following culture result:
    PositiveaNegativeTotal
    PositiveAMTDT 1
     Positive48048
     Negative019b 19
     Total481967
    AMTDT 2
     Positive48048
     Negative019b 19
     Total481967
    NegativeAMTDT 1
     Positive34034
     Negative13296c 309
     Total47296343
    AMTDT 2
     Positive42042
     Negative5296c 301
     Total47296343
    TotalAMTDT 1
     Positive82082
     Negative13315d 328
     Total95315410
    AMTDT 2
     Positive90090
     Negative5315d 320
     Total95315410
    • ↵a Only data for specimens with culture-positive results for M. tuberculosis are included.

    • ↵b Nineteen specimens were AMTDT negative and culture positive for NTM.

    • ↵c Seven specimens were AMTDT negative and culture positive for NTM.

    • ↵d Twenty-six specimens were AMTDT negative and culture positive for NTM.

  • Table 2.

    Comparison of confirmed results for respiratory specimens by AMTDT 1 and AMTDT 2

    Test and resultNo. of specimens with the following confirmed result:Sensitivity (%)Specificity (%)PPV (%)NPV (%)
    PositiveNegativeTotal
    AMTDT 1
     Positive8208283.010010096.0
     Negative13315a 328
     Total95315410
    AMTDT 2
     Positive9009094.710010098.4
     Negative5315a 320
     Total95315410
    • ↵a Twenty-six specimens were culture positive for NTM.

  • Table 3.

    Detection of M. tuberculosis complex in nonrespiratory specimens by initial and new versions of AMTDT

    Microscopy resultTest and resultNo. of specimens with the following culture result:
    PositiveaNegativeTotal
    PositiveAMTDT 1
     Positive21021
     Negative03b 3
     Total21324
    AMTDT 2
     Positive21021
     Negative03b 3
     Total21324
    NegativeAMTDT 1
     Positive36036
     Negative11201c 212
     Total47201248
    AMTDT 2
     Positive38038
     Negative9201c 210
     Total47201248
    TotalAMTDT 1
     Positive57057
     Negative11204d 215
     Total68204272
    AMTDT 2
     Positive59059
     Negative9204d 213
     Total68204272
    • ↵a Only data for specimens with culture-positive results for M. tuberculosis are included.

    • ↵b Three specimens were AMTDT negative and culture positive for NTM.

    • ↵c Six specimens were AMTDT negative and culture positive for NTM.

    • ↵d Nine specimens were AMTDT negative and culture positive for NTM.

  • Table 4.

    Discrepant results obtained for nonrespiratory specimensa

    Type of specimenNo. of specimensAMTDT 1 resultAMTDT 2 resultNo. of specimens inhibiting amplification (test)
    Urine1Pos.Neg.1 (AMTDT 2)
    Urine3Neg.Pos.
    Urine2Neg.Neg.
    Lymph node3Pos.Neg.3 (AMTDT 2)
    Lymph node1Neg.Pos.
    Lymph node1Neg.Neg.
    CSF1Neg.Neg.
    CSF1Neg.Pos.
    Ascitic fluid1Neg.Pos.
    Gastric aspirate1Neg.Neg.
    • ↵a All specimens were negative forM. tuberculosis by microscopy but positive for M. tuberculosis by culture. Neg., negative; Pos., positive.

  • Table 5.

    Comparison of confirmed results for nonrespiratory specimens by AMTDT 1 and AMTDT 2

    Test and resultNo. of specimens with the following confirmed result:Sensitivity (%)Specificity (%)PPV (%)NPV (%)
    PositiveNegativeTotal
    AMTDT 1
     Positive5705783.010010094.0
     Negative11204a 215
     Total68204272
    AMTDT 2
     Positive5905986.810010098.4
     Negative9204a 213
     Total68204272
    • ↵a Nine specimens were culture positive for NTM.

PreviousNext
Back to top
Download PDF
Citation Tools
Comparative Evaluation of Initial and New Versions of the Gen-Probe Amplified Mycobacterium Tuberculosis Direct Test for Direct Detection of Mycobacterium tuberculosis in Respiratory and Nonrespiratory Specimens
Fredy Gamboa, Gregorio Fernandez, Eduardo Padilla, José M. Manterola, Joan Lonca, Pere Joan Cardona, Lurdes Matas, Vicente Ausina
Journal of Clinical Microbiology Mar 1998, 36 (3) 684-689; DOI: 10.1128/JCM.36.3.684-689.1998

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Print

Alerts
Sign In to Email Alerts with your Email Address
Email

Thank you for sharing this Journal of Clinical Microbiology article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Comparative Evaluation of Initial and New Versions of the Gen-Probe Amplified Mycobacterium Tuberculosis Direct Test for Direct Detection of Mycobacterium tuberculosis in Respiratory and Nonrespiratory Specimens
(Your Name) has forwarded a page to you from Journal of Clinical Microbiology
(Your Name) thought you would be interested in this article in Journal of Clinical Microbiology.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Comparative Evaluation of Initial and New Versions of the Gen-Probe Amplified Mycobacterium Tuberculosis Direct Test for Direct Detection of Mycobacterium tuberculosis in Respiratory and Nonrespiratory Specimens
Fredy Gamboa, Gregorio Fernandez, Eduardo Padilla, José M. Manterola, Joan Lonca, Pere Joan Cardona, Lurdes Matas, Vicente Ausina
Journal of Clinical Microbiology Mar 1998, 36 (3) 684-689; DOI: 10.1128/JCM.36.3.684-689.1998
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Top
  • Article
    • ABSTRACT
    • MATERIALS AND METHODS
    • RESULTS
    • DISCUSSION
    • FOOTNOTES
    • REFERENCES
  • Figures & Data
  • Info & Metrics
  • PDF

KEYWORDS

bacteriological techniques
Mycobacterium tuberculosis
Respiratory System
tuberculosis
Tuberculosis, Pulmonary

Related Articles

Cited By...

About

  • About JCM
  • Editor in Chief
  • Board of Editors
  • Editor Conflicts of Interest
  • For Reviewers
  • For the Media
  • For Librarians
  • For Advertisers
  • Alerts
  • RSS
  • FAQ
  • Permissions
  • Journal Announcements

Authors

  • ASM Author Center
  • Submit a Manuscript
  • Article Types
  • Resources for Clinical Microbiologists
  • Ethics
  • Contact Us

Follow #JClinMicro

@ASMicrobiology

       

ASM Journals

ASM journals are the most prominent publications in the field, delivering up-to-date and authoritative coverage of both basic and clinical microbiology.

About ASM | Contact Us | Press Room

 

ASM is a member of

Scientific Society Publisher Alliance

 

American Society for Microbiology
1752 N St. NW
Washington, DC 20036
Phone: (202) 737-3600

 

Copyright © 2021 American Society for Microbiology | Privacy Policy | Website feedback

Print ISSN: 0095-1137; Online ISSN: 1098-660X