DOI: 10.1128/JCM.36.6.1604-1607.1998
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- Table 1.
Serovars, stx genotypes, LEE-associated genes, and adhesive and hemolytic properties of bovine Stx-producingE. coli of serogroup O118 (n = 42)
Serovar and strain Clinical status of calf stxgene hlyEHEC HlyEHEC Hybridization with LEE probes A to D Type of adhesion on: Intensity of FAS reactivity ona: HEp-2 cells FCL cells HEp-2 cells FCL cells O118:H16 GS1236-1b No diarrhea stx 1 + + + LA LA ++++ ++ GS1232-1 No diarrhea stx 1 + + + LA LA +++ − GS1161-1b No diarrhea stx 1 + + + LA LA ++ ++ GS1180-1 No diarrhea stx 1 + + + LA LA ++ ++ GS1205-1 No diarrhea stx 1 + + + LA LA ++ + RW2303c Diarrhea stx 1 + + + LA LA ++++ +++ RW2126c Diarrhea stx 1 + + + LA LA +++ +++ RW2125d Diarrhea stx 1 + + + LA LA +++ ++ RW2173 Diarrhea stx 1 + + + LA LA +++ + RW0136 Diarrhea stx 1 + + + LA LA ++ ++++ RW0203 Diarrhea stx 1 + + + LA LA ++ +++ GS1181-1 Diarrhea stx 1 + + + LA LA ++ ++ AS2169 Diarrhea stx 1 + + + LA LA + +++ 340 Diarrhea stx 1 + + + LA LA + ++ RW2199 Diarrhea stx 1 + + + LA LA + ++ 666/89 Diarrhea stx 1 + + + LA LA + + RW2022 Diarrhea stx 1 + + + LA LA + + RW2266 Diarrhea stx 1 + + + LA LA − +++ RW1306e Diarrhea stx 1 + + + LA LA − ++ RW1912 Diarrhea stx 1 + + + LA LA − + RW1362 Diarrhea stx 1 + − + Negative LA − + RW2121 Diarrhea stx 1 − − − Negative LA − − RW1303f Diarrhea stx 1 + + + Negative LA − + RW1366 Diarrhea stx 1 + + + Negative LA ++ ++ RW1319 Diarrhea stx 1 + + + Negative LA + ++ RW1305 Diarrhea stx 1 + + + Negative LA − + RW1817 Diarrhea stx 1 + + + Negative LA − + RW1307e Diarrhea stx 1 +g + + Negative LA − + RW1318 Diarrhea stx 1 + + + Negative Negative ++ + RW1299 Diarrhea stx 1 + + + Negative Negative − + RW1304f Diarrhea stx 1 + + + Negative Negative − + RW2017 Diarrhea stx 1 + + + Negative Negative − − RW1365 Diarrhea stx 1 + − + Negative Negative − ++ RW2116 Diarrhea stx 2 + + + LA LA − +++ RW2039 Diarrhea stx 2 + + + LA LA − + RW2136 Diarrhea stx 2 + + + LA LA − + RW2297d Diarrhea stx 1/2 + + + LA LA ++ +++ RW1911 Diarrhea stx 1/2 + + + LA LA − ++ O118:H− 557/89 Diarrhea stx 1 + + + LA LA + + RW2311 Diarrhea stx 1 + + + LA LA − − RW1302 Diarrhea stx 1 + + + Negative LA − +++ RW2030 Diarrhea stx 2 + + + LA LA − ++ ↵a Numbers of infected cells with actin accumulation. −, FAS negative; +, ≤1% of infected cells positive; ++, >1 to ≤10%; +++, >10 to ≤50%; and ++++, >50 to ≤100%.
↵b Strains GS1236-1 and GS1161-1 were isolated from the same animal.
↵c Strains RW2303 and RW2126 were isolated from the same animal.
↵d Strains RW2125 and RW2297 were isolated from the same animal.
↵e Strains RW1306 and RW1307 were isolated from the same animal.
↵f Strains RW1303 and RW1304 were isolated from the same animal.
↵g Strain RW1307 reacted negatively with PCR probe Ehly1-5 but positively with DNA probe Ehly1-Ehly5.