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Journal of Clinical Microbiology
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Epidemiology

Molecular Epidemiology of Reemergent Vibrio cholerae O139 Bengal in India

Asish Kumar Mukhopadhyay, Arnab Basu, Pallavi Garg, Prasanta K. Bag, Amit Ghosh, S. K. Bhattacharya, Yoshifumi Takeda, G. Balakrish Nair
Asish Kumar Mukhopadhyay
National Institute of Cholera and Enteric Diseases, Beliaghata, Calcutta-700010, and
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Arnab Basu
National Institute of Cholera and Enteric Diseases, Beliaghata, Calcutta-700010, and
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Pallavi Garg
National Institute of Cholera and Enteric Diseases, Beliaghata, Calcutta-700010, and
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Prasanta K. Bag
National Institute of Cholera and Enteric Diseases, Beliaghata, Calcutta-700010, and
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Amit Ghosh
Institute of Microbial Technology, Chandigarh, India, and
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S. K. Bhattacharya
National Institute of Cholera and Enteric Diseases, Beliaghata, Calcutta-700010, and
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Yoshifumi Takeda
Research Institute, International Medical Center of Japan, Shinjuku-ku, Tokyo 162, Japan
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G. Balakrish Nair
National Institute of Cholera and Enteric Diseases, Beliaghata, Calcutta-700010, and
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DOI: 10.1128/JCM.36.7.2149-2152.1998
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    Fig. 1.

    Monthly isolation of various serogroups of V. cholerae from patients hospitalized because of acute secretory diarrhea at the Infectious Diseases Hospital, Calcutta, from January 1996 to December 1997 (J to D, respectively).

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  • Table 1.

    Antibiogram and RFLP analyses of the CTX genetic element of the 17 clinical V. cholerae O139 strains isolated from different parts of India after September 1996

    Strain no.Place of isolationRegionAntibiogramRestriction patterna of CTX genetic elementRibotypeb
    AS258CalcuttaEasternAP-FZ-NM-SMHindIII: 14.0, 9.0, 7.2; BglII: 23.03a
    MD1MidnapurEasternAP-FZ-NM-SMHindIII: 14.0, 9.0, 7.2; BglII: 23.05a
    MD3MidnapurEasternAP-FZ-NM-SMHindIII: 14.0, 9.0, 7.2; BglII: 23.03a
    NPO549NagpurDeccanAP-FZ-NM-SMHindIII: 14.0, 9.0, 7.2; BglII: 23.03a
    YO29YavatmalDeccanAP-FZ-SMHindIII: 14.0, 9.0, 7.2; BglII: 23.03a
    MO585MadrasSouthernAP-FZ-NM-SMHindIII: 14.0, 9.0, 7.23a
    NPO554NagpurDeccanAP-FZ-SMHindIII: 14.0, 9.0, 7.23a
    NPO564NagpurDeccanAP-FZ-NM-SMHindIII: 14.0, 9.0, 7.23a
    YO30YavatmalDeccanAP-FZ-SMHindIII: 14.0, 9.0, 7.23a
    SO58SewagramDeccanAP-FZ-SMHindIII: 14.0, 9.0, 7.23a
    APO16AmravatiDeccanAP-FZ-NM-SMHindIII: 14.0, 9.0, 7.23a
    VO82VelloreSouthernAP-FZ-NA-SMHindIII: 20.0; BglII: 8.2, 7.23a
    VO83VelloreSouthernAP-FZ-NA-SMHindIII: 20.0; BglII: 8.2, 7.23a
    PO42PuneWesternAP-FZ-NA-SMHindIII: 20.0; BglII: 8.2, 7.25a
    WSO10SolapurDeccanAP-FZ-NA-SMHindIII: 20.0; BglII: 8.2, 7.25a
    BOM6BombayWesternAP-FZ-NM-NA-SMHindIII: 15; BglII: 7.25a
    MO579MadrasSouthernAP-FZ-SMHindIII: 153a
    • ↵a Restriction enzyme and sizes (in kilobases) of the fragments of the CTX genetic element of V. cholerae O139 which hybridized with the ctxA gene probe.

    • ↵b Ribotype designations are the same as those of Popovic et al. (16).

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Molecular Epidemiology of Reemergent Vibrio cholerae O139 Bengal in India
Asish Kumar Mukhopadhyay, Arnab Basu, Pallavi Garg, Prasanta K. Bag, Amit Ghosh, S. K. Bhattacharya, Yoshifumi Takeda, G. Balakrish Nair
Journal of Clinical Microbiology Jul 1998, 36 (7) 2149-2152; DOI: 10.1128/JCM.36.7.2149-2152.1998

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Molecular Epidemiology of Reemergent Vibrio cholerae O139 Bengal in India
Asish Kumar Mukhopadhyay, Arnab Basu, Pallavi Garg, Prasanta K. Bag, Amit Ghosh, S. K. Bhattacharya, Yoshifumi Takeda, G. Balakrish Nair
Journal of Clinical Microbiology Jul 1998, 36 (7) 2149-2152; DOI: 10.1128/JCM.36.7.2149-2152.1998
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KEYWORDS

Cholera
Vibrio cholerae

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