DOI: 10.1128/JCM.37.1.221-224.1999
Article Figures & Data
Figures
- Fig. 1.
RAPD patterns generated by arbitrarily primed PCR of the 22 clones of S. pneumoniae with two primers, M13 and ERIC1. Molecular sizes (lane M) are indicated in kilobases (Gibco BRL).
- Fig. 2.
PFGE profiles of SmaI-digested chromosomal DNA of nine clones of serotype 23F S. pneumoniae isolates. Lane M is a molecular size standard for a bacteriophage λ ladder (Gibco BRL); lanes 1 to 9 show clones 1 to 9, respectively.
Tables
- Table 1.
Characteristics of 67 penicillin- and erythromycin-resistant S. pneumoniae isolates
Clone (no. of isolates) Antibiotype Serotype RAPD patterna Pulsotype Fingerprint profile Source(s) (no. of isolates)b pbp1a pbp2x pbp2b 1 (16) A 23F A a I I I Blood (4), CSF (1), S/B (8), throat (3) 2 (4) B 23F B b II II II Blood (2), S/B (1), sinus (1) 3 (3) C 23F C c III II III Blood (1), S/B (2) 4 (1) B 23F D d IV II IV S/B (1) 5 (1) D 23F E e III II IV Nose (1) 6 (1) E 23F F f V II III Throat (1) 7 (1) F 23F G g VI II V S/B (1) 8 (1) A 23F H h IV II V S/B (1) 9 (6) B 23F I i III II IV Blood (1), S/B (4), nose (1) 10 (2) G 23F J j VII II III Blood (1), S/B (1) 11 (1) H 23F K k III III IV Sinus (1) 12 (1) I 23F L l IV II III S/B (1) 13 (1) B 23F M m III IV III S/B (1) 14 (1) J 23F N n IV II III Pus (1) 15 (4) K 19F O o VI II IV Pus (1), S/B (2), ear (1) 16 (1) A 19F P p IV III III S/B (1) 17 (2) L 19F Q q IV II IV Blood (1), pus (1) 18 (2) M 19F R r IV V V Blood (1), S/B (1) 19 (9) N 19F S s IV II IV S/B (6), wound (2), blood (1) 20 (1) O 19F T t III II V Blood (1) 21 (7) P 6B U u III V IV CSF (2), S/B (2), blood (1), Bile (1), wound (1) 22 (1) Q 6B V v III II V Ear (1) - Table 2.
Antimicrobial susceptibilities of 67 isolates of S. pneumoniae and their antibiotypes
Clone (no. of isolates) MIC (μg/ml) Antibiotype Penicillin Cefotaxime Cefpirome Imipenem Gentamicin Ciprofloxacin Erythromycin 1 (16) 4 8 4 0.5 32 1 ≥256 A 2 (4) 2 2 1 0.5 32 1 ≥256 B 3 (3) 2 4 1 1 16 1 ≥256 C 4 (1) 2 1 1 0.5 32 1 ≥256 B 5 (1) 2 1 1 0.25 8 1 4 D 6 (1) 2 2 1 0.25 32 2 2 E 7 (1) 2 4 2 1 32 1 4 F 8 (1) 8 8 4 0.5 32 2 ≥256 A 9 (6) 2 2 0.5 0.25 16 1 ≥256 B 10 (2) 0.5 1 0.25 1 16 1 2 G 11 (1) 0.12 0.06 ≤0.03 ≤0.03 16 1 ≥256 H 12 (1) 1 4 0.12 0.5 32 1 64 I 13 (1) 1 1 1 0.25 16 0.5 ≥256 B 14 (1) 0.5 0.5 0.06 ≤0.03 0.5 0.12 8 J 15 (4) 2 0.5 ≤0.03 1 0.5 1 2 K 16 (1) 8 8 4 0.5 32 2 ≥256 A 17 (2) 0.25 0.12 ≤0.03 ≤0.03 0.5 0.25 2 L 18 (2) 0.12 0.06 0.06 ≤0.03 16 0.5 16 M 19 (9) 0.25 0.25 0.06 0.06 0.5 1 ≥256 N 20 (1) 0.5 2 0.5 0.12 16 1 1 O 21 (7) 0.5 1 0.5 0.06 2 0.25 32 P 22 (1) 0.5 1 0.5 0.12 1 2 4 Q
