Direct Detection of Shiga Toxigenic Escherichia coliStrains Belonging to Serogroups O111, O157, and O113 by Multiplex PCR

O113-specific PCR.The rfb region of E. coli O113 contains nine genes which may be cotranscribed. Comparison with sequence databases identified candidate genes for four glycosyl transferases, an O-acetyl transferase, an O-unit flippase, and an O-antigen polymerase, as well as copies ofgalE and gnd (18). A portion of the O113 O-antigen polymerase gene (wzy) was the preferred target for a serogroup-specific PCR assay, because it shows the lowest degree of sequence homology with any genes submitted to GenBank (18). Moreover, the fact that the O-antigen polymerase must exhibit absolute specificity for both the oligosaccharide repeat unit and the type of glycosidic linkage formed during polymerization renders the existence of homologous sequences in other organisms extremely unlikely. The O113-specific PCR primers used were 5′-AGCGTTTCTGACATATGGAGTG-3′ and 5′-GTGTTAGTATCAAAAGAGGCTCC-3′ (designated O113F and O113R, respectively). These direct amplification of a 593-bp portion of the O113 wzy gene (nucleotides 3690 to 4282 in the published sequence [18]).

Crude DNA extracts were then prepared from 40 STEC strains in our collection, as described previously (19). O113:H21 STEC strains included five clinical isolates from South Australia (strains 97MW1, 98NK2, and 98BN1) and New Zealand (strains 1183 and 3848; kindly provided by Jenny Bennett) and a food isolate (strain MW10) from South Australia. The other STEC strains tested included serogroups O157 (5 isolates); O111 (7 isolates); O26 and OX3 (2 isolates each); O48, O91, O98, O123, O128, O141, and O159 (1 isolate each); and 11 STEC strains which were O nontypeable. Samples (2 μl) of each extract were amplified in 50-μl reaction mixtures containing 200 μM deoxynucleoside triphosphates (dNTPs), approximately 250 nM each primer (O113F and O113R, as well as the O111- and O157-specific primers designated O111F, O111R, O157F, and O157R [17]), and 1 U of Taq polymerase (Boehringer Mannheim, Mannheim, Germany) in a mixture of 10 mM Tris-HCl (pH 8.3), 50 mM KCl, 2 mM MgCl₂, 0.1% gelatin, 0.1% Tween 20, and 0.1% NP-40. Samples were subjected to 35 PCR cycles, each consisting of 1 min of denaturation at 95°C and 2 min of annealing at 65°C for the first 10 cycles, with decreases in decrements to 60°C by cycle 15, and 1.5 min of elongation at 72°C, with increases in increments to 2.5 min from cycles 25 to 35. PCR mixtures were electrophoresed on 2% agarose gels and stained with ethidium bromide. Extracts from all six O113 STEC isolates yielded 593-bp PCR products (Fig. 1, lanes 2 to 7), whereas extracts from the O111 and O157 STEC strains yielded 406- and 259-bp PCR products, respectively (results for three strains of each serogroup are shown in Fig. 1, lanes 8 to 13). Extracts from the 22 remaining STEC strains were PCR negative (results not presented).

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Fig. 1.

Analysis of reference STEC strains by multiplex PCR. Lanes: M, DNA size markers (pUC19 DNA digested with HpaII; fragment sizes visible are 501/489, 404, 331, 242, 190, 147, and 111 bp); 1, negative control; 2 to 7, O113:H21 STEC strains MW10, 98NK2, 98BN1, 97MW1, 1183, and 3848, respectively; 8 to 10, O111:H⁻ STEC strains 96RO1, 95JB1, and PH, respectively; 11 to 13, O157:H⁻ STEC strains 96GR1, 96/0629, and 95SF2, respectively. The expected mobilities for the various serogroup-specific PCR products are also indicated.

Sensitivity of O113-specific PCR.To assess the sensitivity of the O113-specific PCR, a fresh overnight broth culture of E. coli K-12 was spiked with serial 10-fold dilutions of a broth culture of the O113:H21 STEC strain 98NK2. Extracts of these samples were then subjected to the multiplex PCR assay (Fig. 2). A 593-bp PCR could still be seen in the sample that contained 10⁶-fold-diluted STEC culture (equivalent to <10² STEC CFU per assay), but not in the sample containing 10⁷- or 10⁸-fold dilutions.

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Fig. 2.

Sensitivity of O113-specific PCR. A culture of E. coli K-12 was spiked with serial 10-fold dilutions of a culture of O113:H21 STEC strain 98NK2, and extracts of these samples were then subjected to the rfb-specific PCR assay. Lanes: M, DNA size markers (pUC19 DNA digested with HpaII; fragment sizes visible are 501/489, 404, 331, and 242 bp); 1, negative control (unspiked E. coli K-12 extract); 2 to 9, extracts ofE. coli K-12 culture spiked with 10¹-, 10²-, 10³-, 10⁴-, 10⁵-, 10⁶-, 10⁷-, and 10⁸-fold-diluted 98NK2 cultures, respectively. The expected mobility for the O113-specific PCR product is also indicated.

Analysis of primary fecal cultures.To demonstrate the diagnostic utility of the assay, crude DNA extracts of primary fecal cultures from 22 patients with HUS or bloody diarrhea were examined. Each of these samples had previously tested positive by PCR for the presence of stx₁ and/orstx₂ genes. Three of the original fecal cultures had yielded an O113 STEC isolate, while five yielded O111, and four yielded O157 STEC isolates. All but one of the remainder yielded STEC belonging to other serogroups. Extracts from all three O113 culture-positive samples yielded a 593-bp PCR product (Fig. 3A, lanes 2 to 4). One of these was from a patient with HUS, one was from a patient with diarrhea complicated by microangiopathic hemolytic anemia and thrombocytopenia, and the third was from a patient with bloody diarrhea. A previously culture negative, but stx₂-positive sample from a patient with HUS who had serological evidence of O113 infection (20) was also positive for the O113-specific sequences by PCR (Fig. 3A, lane 5). The extracts from fecal cultures which yielded O157 or O111 STEC isolates also were positive for the appropriate serogroup by PCR, generating 259- or 406-bp PCR products, respectively. (Results for two extracts for each serogroup are shown in Fig. 3A, lanes 6 to 9.) No PCR products were observed for any of the nine other stx-positive extracts from cultures which yielded STEC isolates belonging to other serogroups (results not presented). A total of 30 fecal cultures from patients with diarrhea which had tested negative by PCR forstx were also tested in the serogroup-specific PCR assay. As expected, all of these extracts were also negative in the O111/O113/O157 PCR assay (results for 10 of these are shown in Fig.3B).

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Fig. 3.

Multiplex PCR analysis of primary fecal cultures. (A) Crude DNA extracts of stx-positive primary fecal cultures analyzed by using the rfb-specific PCR assay. Lanes: M, DNA size markers (pUC19 DNA digested with HpaII; fragment sizes visible are 501/489, 404, 331, 242, 190, 147, and 111 bp); 1, negative control; 2 to 4, extracts from three patients with culture-proven O113:H21 STEC infection; 5, extract from anstx₂-positive, but culture-negative HUS patient with serological evidence of O113 infection; 6 and 7, extracts from patients with culture-proven O157:H⁻ STEC infection; 8 and 9, extracts from patients with culture-proven O111:H⁻ STEC infection. (B) Crude DNA extracts of stx-negative primary fecal cultures analyzed by using the rfb-specific PCR assay. Lanes: M, DNA size markers; 2 to 10, extracts ofstx-negative primary fecal cultures; 11, positive control (pooled DNA extracts from reference O157, O111, and O113 STEC strains). The expected mobilities for the various serogroup-specific PCR products are also indicated.

When tested in our previously described multiplex PCR assay for STEC virulence factor genes (17), the O113-positive fecal culture extracts were all positive for stx₂ and EHEC-hlyA; three of the four O157-positive samples were positive for stx₂, eae, and EHEC-hlyA; all of the O111-positive and one of the O157-positive samples were positive for stx₁,stx₂, eae, and EHEC-hlyA. Of the samples yielding STEC isolates belonging to other serogroups, four were O26, and all of these were positive forstx₁, eae, and EHEC-hlyA. The remaining five samples yielded isolates which were alleae negative; one was serogroup O23, while the others were nontypeable. In all of the above cases, the multiplex PCR profile of the crude extracts matched that of the STEC strain isolated from that sample (results not presented).

Discussion and conclusions.PCR is generally considered to be the most sensitive means of determining whether a fecal specimen or a food sample contains STEC (21). Although direct extracts of feces or foods can be used as templates for PCR, the best results are usually obtained by testing extracts of primary broth cultures (2, 9, 19, 21). Broth enrichment serves two purposes: inhibitors in the sample are diluted, and bacterial growth increases the number of copies of the target sequence. Detection of eitherstx₁ or stx₂ genes confirms the presence of STEC, but valuable additional information about the infecting strain can be obtained by testing for the presence of genes encoding putative accessory virulence factors, such as intimin or the plasmid-encoded hemolysin (10, 17, 22). Sequence differences between the eae genes from O157 STEC and othereae-positive serogroups such as O111 have also been used to develop PCR assays capable of recognizing STEC belonging to these serogroups (8, 14). However, in both studies, the O157eae primers also reacted with E. coli O55, an enteropathogenic E. coli serogroup closely related to O157 STEC. Furthermore, not all O111 STEC strains tested reacted with the O111 eae primers (14). The availability of sequence data for the rfb regions of E. coli O111 and O157 (1, 4) has recently enabled development of a multiplex PCR assay with absolute specificity for these two important STEC serogroups (17).

O113 was one of the first STEC serogroups to be associated with HUS (13) and is among the most common of theeae-negative STEC types isolated from cases of human disease (12, 21). This is in spite of the fact that the incidence of infections with STEC serogroups other than O157 may be underestimated. Such strains cannot be recognized by culture on sorbitol MacConkey agar and require more sophisticated diagnostic strategies (21). O113 STEC strains are also prevalent in cattle (3, 5, 16), and so there is ample scope for their entry into the human food chain. In the present study, we have designed PCR primers based on thewzy gene from the O113 rfb locus. These have been combined with the primers specific for regions of the O111 and O157rfb loci in a multiplex format. The various primers were designed such that the serogroup-specific PCR products differ in size (259, 406, and 593 bp for O157, O111, and O113, respectively), and so can be readily distinguished by agarose gel electrophoresis. The specificity of the O113 primers was confirmed by testing DNA extracted from a wide range of STEC strains of a known serogroup, as well as by testing extracts of crude fecal cultures from diarrhea patients without evidence of STEC infection. The utility of this assay for direct detection of O113 sequences in primary fecal cultures from patients infected with O113 STEC was also confirmed. The O113-specific PCR assay was also very sensitive, and a culture spiked with 10⁶-fold-diluted O113 STEC (i.e., STEC comprised 0.0001% of the total flora) generated a PCR product which was visible on an ethidium bromide-stained agarose gel.

The trivalent serogroup-specific multiplex PCR assay described in this paper is clearly a useful adjunct to our previously described PCR forstx₁, stx₂,eae, and EHEC-hlyA (17). Collectively, these two assays can provide comprehensive information about the genotype of an infecting STEC strain within 24 h of receipt of a specimen. Detection of a similar PCR profile in crude fecal extracts from more than one patient within a given period of time may provide the earliest (albeit circumstantial) evidence for a link between cases consistent with a common source outbreak. Confirmation of an outbreak would ultimately depend on isolation and genotyping of the causative STEC, but this may take weeks. Moreover, given the sensitivity of PCR screens, there is a likelihood that a proportion of genuine STEC PCR-positive specimens will not yield an isolate even after heroic efforts. However, knowledge of the serogroup of the infecting STEC strain obtained by multiplex PCR analysis would greatly increase the probability of isolating an STEC strain, because it would enable the deployment of immunomagnetic enrichment techniques using beads coated with antibodies to the respective O antigen.