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Virology

Adenovirus Strains of Subgenus D Associated with Nosocomial Infection as New Etiological Agents of Epidemic Keratoconjunctivitis in Japan

Satoshi Takeuchi, Norihiko Itoh, Eiichi Uchio, Keico Tanaka, Noriko Kitamura, Hikaru Kanai, Kazumi Isobe, Koki Aoki, Shigeaki Ohno
Satoshi Takeuchi
Department of Ophthalmology, Yokohama City University School of Medicine, Kanazawa-ku, Yokohama 236-0004, and
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Norihiko Itoh
Department of Ophthalmology, Yokohama City University School of Medicine, Kanazawa-ku, Yokohama 236-0004, and
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Eiichi Uchio
Department of Ophthalmology, Yokohama City University School of Medicine, Kanazawa-ku, Yokohama 236-0004, and
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Keico Tanaka
Department of Ophthalmology, Yokohama City University School of Medicine, Kanazawa-ku, Yokohama 236-0004, and
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Noriko Kitamura
Department of Ophthalmology, Yokohama City University School of Medicine, Kanazawa-ku, Yokohama 236-0004, and
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Hikaru Kanai
Department of Ophthalmology, Yokohama City University School of Medicine, Kanazawa-ku, Yokohama 236-0004, and
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Kazumi Isobe
Department of Ophthalmology, Yokohama City University School of Medicine, Kanazawa-ku, Yokohama 236-0004, and
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Koki Aoki
Aoki Eye Clinic, Shiroishi-ku, Sapporo 003-0027, Japan
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Shigeaki Ohno
Department of Ophthalmology, Yokohama City University School of Medicine, Kanazawa-ku, Yokohama 236-0004, and
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DOI: 10.1128/JCM.37.10.3392-3394.1999
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ABSTRACT

Adenovirus strains of a new type were isolated from patients with epidemic keratoconjunctivitis. They were not completely neutralized by any antiserum against adenovirus prototypes. PCR followed by restriction endonuclease analysis demonstrated that they were type 8. PCR followed by sequencing revealed a high homology rate between them and type 9.

At present there are 49 serotypes of adenovirus (Ad) (5, 19, 24), and they have been classified into six subgenera, A to F (22). Ad group has been shown to cause upper and lower respiratory tract infections, keratoconjunctivitis, and hemorrhagic cystitis (8). Ad3 and -4 are the most common causes of sporadic keratoconjunctivitis and pharyngoconjunctival fever (PCF), and Ad8, -19, and -37 have been responsible for sporadic cases as well as outbreaks of severe epidemic keratoconjunctivitis (EKC) in several countries, especially in east and southeast Asia, including Japan (1-4, 7). Ad8, -19, and -37 are also well known to be etiological agents for nosocomial infections (9, 12, 23).

In the autumn of 1996, we encountered a case of nosocomial infection with severe keratoconjunctivitis in the ophthalmology ward of Yokohama City University Hospital. To determine the serotype of this newly isolated Ad, two new molecular biological techniques developed by us were performed (16, 20) in addition to the standard neutralization test (NT). Conjunctival swabs were taken from eight patients with acute follicular conjunctivitis within 7 days of onset. Swabs from the conjunctiva of symptomatic eyes were collected and inoculated onto HEp-2 cells and A549 cells, which were examined for the presence of a cytopathic effect (CPE) up to 28 days. Infected cells were also identified by the fluorescent-antibody technique using mouse monoclonal antibody against Ad (Chemicon International, Inc., Temecula, Calif.), herpes simplex virus type 1 (HSV-1), HSV-2 (Micro Trak; Syva Co., Palo Alto, Calif.), and Chlamydia (Micro Trak; Syva Co.).

NT was performed by the microtiter method using HEp-2 cells and A549 cells to provide an index of CPE. All Ad prototypes and antisera against Ad1–11, -14, -19, -22, -34, -35, -37, -40, and -41 were obtained from the American Type Culture Collection (Rockville, Md.).

DNA preparation and PCR-restriction fragment length polymorphism (RFLP) analysis from the conjunctival scrapings were performed according to the method previously developed by us (16). Briefly, nested PCR was performed to amplify the 956-bp DNA fragment of the hexon conserved region. Then, differences in the restriction patterns with three restriction enzymes, EcoT14I, HaeIII, andHinfI, were combined, and the serotypes of the clinical specimens were identified by comparison with Ad prototypes.

A new method developed by us was also used to determine the serotypes (20). This method detects Ad and identifies the serotype using a combination of nested PCR and direct sequencing method targeting hypervariable regions (HVRs) that participate in serotype-specific neutralization. Direct sequencing was carried out for the nested PCR products containing seven HVRs and these sequences were determined.

Sequence data described in this article have been deposited with the GenBank/EMBL/DDBJ Data Libraries under the following accession numbers. For subgenus B2: Ad11 hexon gene, AB018424 ; Ad14, AB018425 ; Ad34, AB018426 ; Ad35, AB018427 . For subgenus D: Ad8, AB023546 ; Ad9,AB023547 ; Ad10, AB023548 ; Ad17, AB023549 ; Ad19, AB023550 ; Ad22,AB023551 ; Ad23, AB023552 ; Ad24, AB023553 ; Ad26, AB023554 ; Ad37,AB023555 ; Ad45, AB023556 ; Ad46, AB023557 ; Ad47, AB023558 . The amino acid sequences of these residues were deduced. Deduced amino acid sequences for adenoviruses (with accession numbers and references) taken from previous reports were as follows. Those for subgenus B1 were Ad3, X76549 (15), and Ad7, X76551 (14). Those for subgenus C were Ad1, X67709 (13); Ad2, J 01917 (10); Ad5, X76550 (11); and Ad6, X67710 (13). That for subgenus E was Ad4, X84646 (15). Those for subgenus F were Ad40, X51782 (22), and Ad41,X51783 (21).

Virus was isolated from three of eight patients’ clinical samples. Cultures were subpassaged several times, and it took more than 3 weeks to obtain CPE of 4+ in cultured cells. HEp-2 and A549 cell cultures harvested 2 days after showing 4+ CPE were subjected to various tests for Ad infection. Cultures exhibiting 3+ to 4+ CPE were stained with diluted group-specific anti-adenovirus hexon monoclonal antibody by direct fluorescence-antibody test.

In NT tests, these isolates were not significantly neutralized with rabbit immune serum to the Ad prototype used in this study. However, weak reactions between these isolates and Ad8 or Ad9 were observed. The serotype of these isolates was tentatively named Ad8/9. Antiserum against Ad9 gave titers 128-fold lower against these strains than against Ad9 prototype antigen. On the other hand, antiserum against Ad8 gave titers 16-fold lower against these strains than against Ad8 prototype antigen.

The PCR-RFLP cleavage patterns of the eight clinical samples were identical and showed the same patterns as those of Ad8 prototype (Fig.1). The restriction enzyme patterns of clinical samples were different from those of Ad9 prototype with two of three enzymes, EcoT14I and HaeIII.

Fig. 1.
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Fig. 1.

Agarose gel electrophoresis shows the cleavage patterns of the 956-bp amplified products digested by EcoT14I (a),HaeIII (b), and HinfI (c). Numbers above the lanes correspond to serotypes of Ad and lane M contains molecular weight standards (φX174 HincII digest). Among subgenus D, we could distinguish seven serotypes of the Ad prototype employed in this study, Ad8, -9, -10, -17, -19, -22, and -37, with two enzymes,EcoT14I and HaeIII.

The amino acid sequences of the hexon HVR genes derived from the nucleotide sequences of the eight clinical samples were identical. The clinical isolates, Ad8/9, had a higher average homology in the hexon HVRs with subgenus D serotypes than with other serotypes. This indicates that these strains belong to the subgenus D. Among the serotypes of subgenus D, Ad8/9 had the highest mean maximum homology with Ad9 prototype, 75.0%. In five out of seven HVRs, HVR1, -2, -3, -5, and -7, the highest DNA homology was seen between Ad8/9 and Ad9, 65.7, 94.1, 90.9, 76.2, and 81.8%, respectively (Table1).

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Table 1.

Hexon protein sequence homology between Ad8/9 and adenovirus subgenus D

In this study, we isolated three strains of an uncommon Ad which differed from established Ads as a causative agent of conjunctivitis from eight patients diagnosed clinically as having EKC. The fact that our present strain showed a weak reaction with rabbit hyperimmune serum to both Ad8 and Ad9 means this strain may be an intermediate strain between Ad8 and Ad9, called intermediate-type virus, Ad8/9. It may have originated by recombination of Ad8 and Ad9 within the neutralization related antigen. PCR-RFLP analysis using the conserved region of the hexon identified this Ad8/9 strain as Ad8. These results suggest that this strain is most similar to Ad8 in whole hexon region and has partial replacement in the area of the neutralization epitopes, that is, HVR1 to HVR7, leading to a sequence similar to that of Ad9. From the viewpoint of the evolution of Ads, it is interesting to speculate on the origin of this strain. The recombination in the hexon region seen in this Ad8/9 has not been previously reported, and simultaneous outbreaks caused by the two Ad serotypes have been reported (18).

In the PCR-sequencing method using seven HVRs, Ad8/9 had the highest mean maximum homology with Ad9 prototype, 75.0%. However, this rate is not so high. These results support the finding that Ad8/9 was not neutralized completely by antiserum against Ad9. In our previous study, the predicted amino acid homology of these HVRs suggested three regions, HVR4, -5, and -7, to be good candidates for neutralization epitopes, because of their low homology among human Ads (20). In fact, the average homology rate of HVR4, -5, and -7, 76.5%, indicated that Ad8/9 was most closely related to Ad9. The highest average homology in HVR4, -5, and -7, between Ad8/9 and Ad9, strongly supported the weak reaction in NT with rabbit immune serum to Ad9.

As a result of the sudden emergence of nosocomial infection in association with conjunctivitis, it would be of interest to investigate whether the properties of Ad8/9 vary from those of Ad8 and Ad9 prototypes and whether it is serologically related to the other Ad associated with conjunctivitis. It remains to be determined whether this Ad8/9 is a new Ad serotype, candidate Ad50.

Some patients showed corneal involvement in the form of subepithelial punctate keratitis and enlarged preauricular lymph nodes, which is commonly seen in Ad8 infection (1, 7). The conjunctival symptoms of acute follicular conjunctivitis were not similar to those of PCF, but were similar to those of EKC. EKC caused by subgenus D, Ad8, Ad19, and Ad37 has been reported to be associated with many cases of nosocomial infection (1, 2, 4, 6). In the case of infection in our hospital, it was shown that the degree of contagiousness or pathogenicity for this serotype, Ad8/9, is the same as that for Ad8, Ad19, and Ad37, belonging to subgenus D.

In our previous survey, we did not detect this new serotype in Japan and east Asia (1-3, 7, 17). Thus, the seroprevalence of this serotype in the general population is estimated to be low and outbreaks of adenoviral conjunctivitis caused by this serotype in Japan should be taken into consideration.

ACKNOWLEDGMENTS

The skillful technical assistance provided by S. Matayoshi, Y. Yamada, K. Kanai, M. Kishikawa, H. Maejima, Y. Mochida, and A. Ikeda is gratefully acknowledged.

FOOTNOTES

    • Received 5 March 1999.
    • Returned for modification 25 May 1999.
    • Accepted 5 July 1999.
  • Copyright © 1999 American Society for Microbiology

REFERENCES

  1. 1.↵
    1. Aoki K.,
    2. Kato M.,
    3. Ohtsuka H.,
    4. Ishii K.,
    5. Nakazono N.,
    6. Sawada H.
    Clinical and aetiological study of adenoviral conjunctivitis, with special reference to adenovirus type 4 and 19 infections.Br. J. Ophthalmol.661982776780
    OpenUrlAbstract/FREE Full Text
  2. 2.↵
    1. Aoki K.,
    2. Kawana R.,
    3. Matsumoto I.,
    4. Wadell G.,
    5. de Jong J. C.
    Viral conjunctivitis with special reference to adenovirus type 37 and enterovirus 70 infection.Jpn. J. Ophthalmol.301986158164
    OpenUrlPubMed
  3. 3.↵
    1. Guo D. F.,
    2. Shinagawa M.,
    3. Aoki K.,
    4. Sawada H.,
    5. Itakura S.,
    6. Sato G.
    Genome typing of adenovirus strains isolated from conjunctivitis in Japan, Australia, and the Philippines.Microbiol. Immunol.32198811071118
    OpenUrlCrossRefPubMed
  4. 4.↵
    1. Hierholzer J. C.,
    2. Guyer B.,
    3. O’Day D. M.,
    4. Shaffner W.
    Adenovirus type 19 keratoconjunctivitis.N. Engl. J. Med.29019741436
    OpenUrlPubMedWeb of Science
  5. 5.↵
    1. Hierholzer J. C.,
    2. Wigand R.,
    3. Anderson L. J.,
    4. Adrian T.,
    5. Gold J. W. M.
    Adenoviruses from patients with AIDS: a plethora of serotypes and a description of five new serotypes of subgenus D (type 43–47).J. Infect. Dis.1581988804813
    OpenUrlCrossRefPubMed
  6. 6.↵
    1. Horwitz M. S.
    Adenoviruses Fields virology 3rd ed. Fields B. N., Knipe D. M., Howley P. M. 1996 2149 2171 Lippincott-Raven Publishers Philadelphia, Pa
  7. 7.↵
    1. Ishii K.,
    2. Nakazono N.,
    3. Fujinaga K.,
    4. Fujii S.,
    5. Kato M.,
    6. Ohtsuka H.,
    7. Aoki K.,
    8. Chen C. W.,
    9. Lin C. C.,
    10. Sheu M. M.,
    11. Lin K. H.,
    12. Oum B. S.,
    13. Lee S. H.,
    14. Chun C. H.,
    15. Yoshii T.,
    16. Yamazaki S.
    Comparative studies on aetiology and epidemiology of viral conjunctivitis in three countries of east Asia—Japan, Taiwan and South Korea.Int. J. Epidemiol.16198798103
    OpenUrlCrossRefPubMedWeb of Science
  8. 8.↵
    1. Jackson G. G.,
    2. Muldoon R. L.
    Viruses causing common respiratory infection in man. IV. Reoviruses and adenoviruses.J. Infect. Dis.1281973811866
    OpenUrlCrossRefPubMed
  9. 9.↵
    1. Jernigan J. A.,
    2. Lowry B. S.,
    3. Hayden F. G.,
    4. Kyger S. A.,
    5. Conway B. P.,
    6. Gröschel D. H. M.,
    7. Farr B. M.
    Adenovirus type 8 epidemic keratoconjunctivitis in an eye clinic: risk factors and control.J. Infect. Dis.167199313071313
    OpenUrlCrossRefPubMed
  10. 10.↵
    1. Jörnvall H.,
    2. Akusjärvi G.,
    3. Aleström P.,
    4. von Bahr-Lindström H.,
    5. Pettersson U.,
    6. Appella E.,
    7. Fowler A. V.,
    8. Philipson L.
    The adenovirus hexon protein. The primary structure of the polypeptide and its correlation with the hexon gene.J. Biol. Chem.256198161816186
    OpenUrlAbstract/FREE Full Text
  11. 11.↵
    1. Kinloch R.,
    2. Mackay N.,
    3. Mautner V.
    Adenovirus hexon: sequence comparison of subgroup C serotypes 2 and 5.J. Biol. Chem.259198464316436
    OpenUrlAbstract/FREE Full Text
  12. 12.↵
    1. Nauheim R. C.,
    2. Romanowski E. G.,
    3. Araullo-Cruz T.,
    4. Kowalski R. P.,
    5. Turgeon P. W.,
    6. Stopak S. S.,
    7. Gordon Y. J.
    Prolonged recoverability of desiccated adenovirus type 19 from various surfaces.Ophthalmology97199014501453
    OpenUrlPubMedWeb of Science
  13. 13.↵
    1. Pring-Åkerblom P.,
    2. Adrian T.
    The hexon genes of adenoviruses of subgenus C: comparison of the variable regions.Res. Virol.1441993117127
    OpenUrlPubMedWeb of Science
  14. 14.↵
    1. Pring-Åkerblom P.,
    2. Trijssenaar F. E. J.,
    3. Adrian T.
    Hexon sequence of adenovirus type 7 and comparison with other serotypes of subgenus B.Res. Virol.1461995383388
    OpenUrlCrossRefPubMed
  15. 15.↵
    1. Pring-Åkerblom P.,
    2. Trijssenaar F. E. J.,
    3. Adrian T.
    Sequence characterization and comparison of human adenovirus subgenus B and E hexons.Virology2121995232236
    OpenUrlCrossRefPubMedWeb of Science
  16. 16.↵
    1. Saitoh-Inagawa W.,
    2. Oshima A.,
    3. Aoki K.,
    4. Itoh N.,
    5. Isobe K.,
    6. Uchio E.,
    7. Ohno S.,
    8. Nakajima H.,
    9. Hata K.,
    10. Ishiko H.
    Rapid diagnosis of adenoviral conjunctivitis by PCR and restriction fragment length polymorphism analysis.J. Clin. Microbiol.34199621132116
    OpenUrlAbstract/FREE Full Text
  17. 17.↵
    1. Saitoh-Inagawa W.,
    2. Aoki K.,
    3. Uchio E.,
    4. Itoh N.,
    5. Ohno S.
    Ten years’ surveillance of viral conjunctivitis in Sapporo, Japan.Graefe’s Arch. Clin. Exp. Ophthalmol.23719993538
    OpenUrlCrossRefPubMedWeb of Science
  18. 18.↵
    1. Schaap G. J. P.,
    2. de Jong J. C.,
    3. van Bijsterveld O. P.,
    4. Beekhuis W. H.
    A new intermediate adenovirus type causing conjunctivitis.Arch. Ophthalmol.97197923362338
    OpenUrlCrossRefPubMedWeb of Science
  19. 19.↵
    1. Schnurr D. P.,
    2. Dondero M. E.
    Two new candidate adenovirus serotypes.Intervirology3619937983
    OpenUrlCrossRefPubMedWeb of Science
  20. 20.↵
    1. Takeuchi S.,
    2. Itoh N.,
    3. Uchio E.,
    4. Aoki K.,
    5. Ohno S.
    Serotyping of adenoviruses on conjunctival scrapings by PCR and sequence analysis.J. Clin. Microbiol.37199918391845
    OpenUrlAbstract/FREE Full Text
  21. 21.↵
    1. Toogood C. I. A.,
    2. Hay R. T.
    DNA sequence of the adenovirus type 41 hexon gene and predicted structure of the protein.J. Gen. Virol.69198822912301
    OpenUrlPubMed
  22. 22.↵
    1. Toogood C. I. A.,
    2. Murali R.,
    3. Burnett R. M.,
    4. Hay R. T.
    The adenovirus type 40 hexon: sequence, predicted structure and relationship to other adenovirus hexons.J. Gen. Virol.70198932033214
    OpenUrlPubMedWeb of Science
  23. 23.↵
    1. Warren D.,
    2. Nelson K. E.,
    3. Farrar J. A.,
    4. Hurwitz E.,
    5. Hierholzer J.,
    6. Ford E.,
    7. Anderson L. J.
    A large outbreak of epidemic keratoconjunctivitis: problems in controlling nosocomial spread.J. Infect. Dis.1601989938943
    OpenUrlCrossRefPubMed
  24. 24.↵
    1. Wigand R.,
    2. Adrian T.,
    3. Bricout F.
    A new human adenovirus of subgenus D: candidate adenovirus type 42.Arch. Virol.941987283286
    OpenUrlCrossRefPubMed
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Adenovirus Strains of Subgenus D Associated with Nosocomial Infection as New Etiological Agents of Epidemic Keratoconjunctivitis in Japan
Satoshi Takeuchi, Norihiko Itoh, Eiichi Uchio, Keico Tanaka, Noriko Kitamura, Hikaru Kanai, Kazumi Isobe, Koki Aoki, Shigeaki Ohno
Journal of Clinical Microbiology Oct 1999, 37 (10) 3392-3394; DOI: 10.1128/JCM.37.10.3392-3394.1999

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Adenovirus Strains of Subgenus D Associated with Nosocomial Infection as New Etiological Agents of Epidemic Keratoconjunctivitis in Japan
Satoshi Takeuchi, Norihiko Itoh, Eiichi Uchio, Keico Tanaka, Noriko Kitamura, Hikaru Kanai, Kazumi Isobe, Koki Aoki, Shigeaki Ohno
Journal of Clinical Microbiology Oct 1999, 37 (10) 3392-3394; DOI: 10.1128/JCM.37.10.3392-3394.1999
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KEYWORDS

Adenoviridae
Cross Infection
Keratoconjunctivitis

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