Article Figures & Data
Figures
- Fig. 1.
PCR-RFLP of B. henselae, B. vinsonii, B. elizabethae, and B. quintana. Lane 1, 50-bp ladder; lanes 2 to 4, B. henselae B91-002000; lanes 5 to 7, B. vinsonii B92-010225; lanes 8 to 10,B. elizabethae B92-002005; lanes 11 to 13, B. quintana ATCC VR-358; lane 14, 50-bp ladder. PCR products were uncut (lanes 2, 5, 8, and 11) or cut with DdeI (lanes 3, 6, 9, and 12) or MseI (lanes 4, 7, 10, and 13).
- Fig. 2.
PCR-RFLP of B. quintana and B. henselae from clinical specimens. Lane 1, 50-bp ladder, lanes 2 to 4, B. quintana H93-176; lanes 5 to 7, B. henselaeB92-007003; lane 8, blank; lanes 9 to 11, B. quintanaB93-007356; lane 12, 50-bp ladder. PCR products were left uncut (lanes 2, 5, and 9) or were cut with DdeI (lanes 3, 6, and 10) orMseI (lanes 4, 7, and 11).
Tables
- Table 1.
Identification of Bartonella species by PCR-RFLP analysis of a 16S rDNA fragment
Bartonellasp. DdeI MseI PCR-RFLP composite pattern Lengths of fragmentsa (bp) No. of restriction sites Length of fragments (bp) No. of restriction sites O P O P B. henselae 190, 115 187, 109 1 214, 81 217, 79 1 I B. vinsonii 158, 114 159, 109, 28 2 218, 82 217, 79 2 II B. elizabethae 154, 110 159, 109, 28 2 166, 79, 52 173, 79, 44 2 III B. quintana 180, 106 187, 109 1 168, 75, 47 173, 79, 44 2 IV ↵a O, observed; P, predicted (GenBank sequences).
