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Bacteriology

PCR Targeted to the 16S-23S rRNA Gene Intergenic Spacer Region ofClostridium difficile and Construction of a Library Consisting of 116 Different PCR Ribotypes

Simon L. J. Stubbs, Jon S. Brazier, Gael L. O’Neill, Brian I. Duerden
Simon L. J. Stubbs
Anaerobe Reference Unit, Department of Medical Microbiology and Public Health Laboratory, University Hospital of Wales, Cardiff, 1 and
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Jon S. Brazier
Anaerobe Reference Unit, Department of Medical Microbiology and Public Health Laboratory, University Hospital of Wales, Cardiff, 1 and
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Gael L. O’Neill
Central Public Health Laboratory, London, 2 United Kingdom
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Brian I. Duerden
Anaerobe Reference Unit, Department of Medical Microbiology and Public Health Laboratory, University Hospital of Wales, Cardiff, 1 and
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DOI: 
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    Fig. 1.

    PCR ribotype profiles obtained with strains belonging to PCR ribotypes 81 to 90. Lane L refers to 100-bp ladder (200 bp − 1 kbp).

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  • Table 1.

    PCR ribotypes of C. difficile depicting type strain, number of isolates obtained to date, and in vitro toxin production

    PCR ribotypeType strainProduction of toxin A/BNo. of isolatesCommenta
    001R8366+/+846Serogroup G
    002R8375+/+60Serogroup A2
    003R8384+/+3
    004R8386+/+1
    005R8373+/+54
    006R8268+/+11
    007R8264+/+1
    008 R10568 +/+2
    009R8269−/−46Serogroup I
    010R8270−/−110Most frequent commu-nity isolate
    011R7619+/+4
    012R6187+/+73
    013R5252+/+12
    014 R11446 +/+104Serogroup H
    015R6685+/+76Serogroup G
    016 R10424 +/+2
    017R7404−/+32Serogroup F
    018R6184+/+10Serogroup A8
    019R8637+/+1
    020 R10079 +/+84Serogroup H
    021R8763+/+5Serogroup A1
    022R4262+/+1
    023R6928+/+43Weak toxin expression
    024R6321+/+11
    025R7276+/+2
    026 R10118 +/+28Weak toxin expression
    027 R12087 +/+1CD196 (binary toxin strain)
    028R9300−/−1
    029R8438+/+3
    030 R11004 −/−8
    031 R11631 −/−24Serogroup K
    032R6598−/−2
    033IS58−/−2Serogroup E6
    034IS81+/+9Serogroup A5
    035 R11812 −/−8
    036CCUG20309−/+1Strain 8864
    037R6641+/+1
    038NCTC11206−/−19Serogroup C
    039 R10738 −/−10Serogroup A10
    040 R10917 −/−1
    041 R10920 −/−1
    042 R11817 +/+6
    043NCTC11382+/+2
    044 R10976 +/+2
    045 R10842 +/+4
    046 R10991 +/+4
    047 R10541 −/+4
    048 R10069 +/+3Weak toxin expression
    049R6320+/+8
    050R9414+/+9
    051R9549−/−1
    052R6155+/+1
    053IS21+/+6Serogroup K
    054IS22+/+15Serogroup A1
    055 R11652 +/+3
    056IS25+/+19
    057IS27+/+5Serogroup K
    058 R10456 +/+12Weak toxin expression
    059R9304+/+1
    060IS40−/−2Serogroup B
    061 R12099 +/+1
    062 R11382 +/+4
    063IS47+/+2Serogroup A5
    064IS48+/+2Serogroup A6
    065IS49−/−2Serogroup A7
    066IS51−/−4Serogroup A9
    067IS52−/−4Serogroup A10
    068IS56−/−1
    069IS59−/−1
    070R9367+/+2Serogroup K
    071IS64−/−1Serogroup S1
    072 R12095 +/+1Serogroup X
    074IS72−/−1
    075IS93+/+1Serogroup A1
    076 R11548 +/+4Serogroup A8
    077 R10955 +/+2
    078R7605+/+13Weak toxin expression
    079R7606−/−1
    081R9764+/+9
    082R7638−/−1
    083 R10566 +/+1Serogroup S1
    084R8768−/−6
    085 R12098 −/−4Serogroup X
    086R1880+/+3
    087 R11840 +/+5
    088 R10855 −/−2
    089R8603−/−1
    090 R10737 +/+1
    091R8643−/−1
    092 R10630 +/+4
    093R8853+/+1
    094 R10078 +/+4
    095R8858+/+1
    096R9759+/+2
    097R8914+/+1
    098R9116−/−2
    099R7425−/−1
    100 R12104 −/−2
    101 R10836 +/+1
    104R9180+/+1
    106 R10459 +/+88
    107R9313+/+1
    110R7771−/+2
    111 R10870 +/+1
    112R8631−/−1
    114 R11212 −/−1
    115 R11244 +/+6Serogroup G
    116 R11347 +/+1
    117 R10071 +/+1
    118 R11394 +/+1
    119 R11805 −/−1
    120 R11830 +/+1
    121R9378−/−1
    122R9385+/+1
    123 R11907 −/−1
    124 R11919 −/−1
    • ↵a Reference where known has been made to Delmee serotype (6).

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PCR Targeted to the 16S-23S rRNA Gene Intergenic Spacer Region ofClostridium difficile and Construction of a Library Consisting of 116 Different PCR Ribotypes
Simon L. J. Stubbs, Jon S. Brazier, Gael L. O’Neill, Brian I. Duerden
Journal of Clinical Microbiology Feb 1999, 37 (2) 461-463; DOI:

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PCR Targeted to the 16S-23S rRNA Gene Intergenic Spacer Region ofClostridium difficile and Construction of a Library Consisting of 116 Different PCR Ribotypes
Simon L. J. Stubbs, Jon S. Brazier, Gael L. O’Neill, Brian I. Duerden
Journal of Clinical Microbiology Feb 1999, 37 (2) 461-463; DOI:
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