Article Figures & Data
Tables
- Table 1.
Percent agreement between colorimetric and NCCLS reference broth microdilution MIC pairsa
Fungus (no. of isolates tested) Antifungal agent % Agreement (range) between MIC pairs at incubation time (h) 24 vs 24 48 vs 48 24 vs 48 C. albicans (465) Amphotericin B 97 97 76 Fluconazole 87 60 71 Flucytosine 87 56 89 Itraconazole 89 54 76 Ketoconazole 90 56 69 Other commonCandida spp. (417)b Amphotericin B 85–97 92–99 40–75 Fluconazole 95–98 73–99 91–99 Flucytosine 92–99 73–100 89–97 Itraconazole 93–100 66–97 86–97 Ketoconazole 93–100 66–97 85–97 Emerging Candida spp. (64)c Amphotericin B 90 90 63 Fluconazole 98 94 97 Flucytosine 95 99 77 Itraconazole 92 91 90 Ketoconazole 95 94 85 C. neoformans (107)d Amphotericin B ND 76 ND Fluconazole ND 98 ND Flucytosine ND 96 ND Itraconazole ND 96 ND Ketoconazole ND 90 ND Miscellaneous yeasts and yeast-like organisms (68)e Amphotericin B 91 99 67 Fluconazole 100 99 99 Flucytosine 99 99 94 Itraconazole 97 95 90 Ketoconazole 94 97 90 ↵a Sensititre YeastOne Colorimetric Antifungal Panel MICs versus NCCLS broth microdilution M27-A MICs. An incubation time of 48 to 96 h was used for C. neoformans and certain emerging yeast pathogens. ND, not determined.
↵b Species included 77 to 95 isolates each ofC. glabrata, C. krusei, C. lusitaniae,C. parapsilosis, and C. tropicalis.
↵c Species included 5 to 26 isolates each ofC. ciferrii, C. famata, C. guilliermondii, C. kefyr, C. lambica,C. lypolytica, C. rugosa, and C. zeylanoides.
↵d The eight non-C. neoformans Cryptococcus isolates tested are not listed.
↵e Species included 2 to 23 isolates each ofB. capitatus, H. anomala, R. rubra,R. minuta, S. cerevisiae, S. salmonicolor, and T. beigelii.
- Table 2.
Percent agreement between YeastOne panel and NCCLS reference method for ranking isolates of common Candidaspp. within the established breakpointsa
Antifungal agent Category and breakpoint (μg/ml)b % Agreement by species (no. of isolates at each breakpoint) C. albicans C. glabrata C. krusei C. lusitaniae C. parapsilosis C. tropicalis Fluconazole S (≤8) 89 (442) 96 (49) NAc 96 (76) 98 (87) 95 (89) S-DD (16–32) 75 (12) 94 (32) NA ND (0) ND (7) ND (2) R (≥64) 93 (14) 100 (14) NA ND (1) ND (1) ND (3) Itraconazole S (≤0.12) 91 (346) ND (5) ND (6) 93 (30) 100 (38) 100 (39) S-DD (0.2–0.5) 24 (104) 97 (40) 100 (49) 98 (43) 100 (54) 89 (48) R (≥1.0) 50 (18) 90 (48) 100 (12) ND (4) ND (3) ND (7) Flucytosine S (≤4) 88 (446) 98 (93) 91 (23) 98 (64) 99 (95) 95 (88) I (8–16) ND (2) ND (0) 100 (44) ND (1) ND (0) ND (2) R (≥32) 75 (20) ND (2) ND (0) 100 (12) ND (0) ND (4)