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Mycology

Multicenter Comparison of the Sensititre YeastOne Colorimetric Antifungal Panel with the National Committee for Clinical Laboratory Standards M27-A Reference Method for Testing Clinical Isolates of Common and Emerging Candidaspp., Cryptococcus spp., and Other Yeasts and Yeast-Like Organisms

A. Espinel-Ingroff, M. Pfaller, S. A. Messer, C. C. Knapp, S. Killian, H. A. Norris, M. A. Ghannoum
A. Espinel-Ingroff
Medical College of Virginia of Virginia Commonwealth University, Richmond, Virginia;
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M. Pfaller
University of Iowa College of Medicine, Iowa City, Iowa; and
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S. A. Messer
University of Iowa College of Medicine, Iowa City, Iowa; and
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C. C. Knapp
AccuMed International, Westlake, and
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S. Killian
AccuMed International, Westlake, and
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H. A. Norris
University Hospital of Cleveland and Case Western Reserve University, Cleveland, Ohio
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M. A. Ghannoum
University Hospital of Cleveland and Case Western Reserve University, Cleveland, Ohio
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DOI: 10.1128/JCM.37.3.591-595.1999
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Tables

  • Table 1.

    Percent agreement between colorimetric and NCCLS reference broth microdilution MIC pairsa

    Fungus (no. of isolates tested)Antifungal agent% Agreement (range) between MIC pairs at incubation time (h)
    24 vs 2448 vs 4824 vs 48
    C. albicans (465)Amphotericin B 979776
    Fluconazole 876071
    Flucytosine 875689
    Itraconazole 895476
    Ketoconazole 905669
    Other commonCandida spp. (417)bAmphotericin B85–97 92–99 40–75
    Fluconazole95–98 73–99 91–99
    Flucytosine92–99 73–10089–97
    Itraconazole93–10066–97 86–97
    Ketoconazole93–10066–97 85–97
    Emerging Candida spp. (64)cAmphotericin B 909063
    Fluconazole 989497
    Flucytosine 959977
    Itraconazole 929190
    Ketoconazole 959485
    C. neoformans (107)dAmphotericin BND76ND
    FluconazoleND98ND
    FlucytosineND96ND
    ItraconazoleND96ND
    KetoconazoleND90ND
    Miscellaneous yeasts and yeast-like organisms (68)eAmphotericin B 919967
    Fluconazole1009999
    Flucytosine 999994
    Itraconazole 979590
    Ketoconazole 949790
    • ↵a Sensititre YeastOne Colorimetric Antifungal Panel MICs versus NCCLS broth microdilution M27-A MICs. An incubation time of 48 to 96 h was used for C. neoformans and certain emerging yeast pathogens. ND, not determined.

    • ↵b Species included 77 to 95 isolates each ofC. glabrata, C. krusei, C. lusitaniae,C. parapsilosis, and C. tropicalis.

    • ↵c Species included 5 to 26 isolates each ofC. ciferrii, C. famata, C. guilliermondii, C. kefyr, C. lambica,C. lypolytica, C. rugosa, and C. zeylanoides.

    • ↵d The eight non-C. neoformans Cryptococcus isolates tested are not listed.

    • ↵e Species included 2 to 23 isolates each ofB. capitatus, H. anomala, R. rubra,R. minuta, S. cerevisiae, S. salmonicolor, and T. beigelii.

  • Table 2.

    Percent agreement between YeastOne panel and NCCLS reference method for ranking isolates of common Candidaspp. within the established breakpointsa

    Antifungal agentCategory and breakpoint (μg/ml)b% Agreement by species (no. of isolates at each breakpoint)
    C. albicansC. glabrataC. kruseiC. lusitaniaeC. parapsilosisC. tropicalis
    FluconazoleS (≤8)89 (442)96 (49)NAc96 (76)98 (87)95 (89)
    S-DD (16–32)75 (12)94 (32)NAND (0)ND (7)ND (2)
    R (≥64)93 (14)100 (14)NAND (1)ND (1)ND (3)
    ItraconazoleS (≤0.12)91 (346)ND (5)ND (6)93 (30)100 (38)100 (39)
    S-DD (0.2–0.5)24 (104)97 (40)100 (49)98 (43)100 (54)89 (48)
    R (≥1.0)50 (18)90 (48)100 (12)ND (4)ND (3)ND (7)
    FlucytosineS (≤4)88 (446)98 (93) 91 (23)98 (64)99 (95)95 (88)
    I (8–16)ND (2)ND (0)100 (44)ND (1)ND (0)ND (2)
    R (≥32)75 (20)ND (2)ND (0)100 (12)ND (0)ND (4)
    • ↵a MICs were read at 24 h for both methods. ND, not determined.

    • ↵b NCCLS breakpoints: S, susceptible; S-DD, susceptible-dose dependent; I, intermediate; R, resistant.

    • ↵c NA, not applicable: the breakpoints do not apply to C. krusei and fluconazole (intrinsically resistant species).

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Multicenter Comparison of the Sensititre YeastOne Colorimetric Antifungal Panel with the National Committee for Clinical Laboratory Standards M27-A Reference Method for Testing Clinical Isolates of Common and Emerging Candidaspp., Cryptococcus spp., and Other Yeasts and Yeast-Like Organisms
A. Espinel-Ingroff, M. Pfaller, S. A. Messer, C. C. Knapp, S. Killian, H. A. Norris, M. A. Ghannoum
Journal of Clinical Microbiology Mar 1999, 37 (3) 591-595; DOI: 10.1128/JCM.37.3.591-595.1999

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Multicenter Comparison of the Sensititre YeastOne Colorimetric Antifungal Panel with the National Committee for Clinical Laboratory Standards M27-A Reference Method for Testing Clinical Isolates of Common and Emerging Candidaspp., Cryptococcus spp., and Other Yeasts and Yeast-Like Organisms
A. Espinel-Ingroff, M. Pfaller, S. A. Messer, C. C. Knapp, S. Killian, H. A. Norris, M. A. Ghannoum
Journal of Clinical Microbiology Mar 1999, 37 (3) 591-595; DOI: 10.1128/JCM.37.3.591-595.1999
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