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Mycobacteriology and Aerobic Actinomycetes

Multicenter Evaluation of the BACTEC MGIT 960 System for Recovery of Mycobacteria

Bruce A. Hanna, Adeleh Ebrahimzadeh, L. Bruce Elliott, Margie A. Morgan, Susan M. Novak, Sabine Rusch-Gerdes, Millie Acio, Denise F. Dunbar, T. Michele Holmes, Charles H. Rexer, Chiminyan Savthyakumar, Ann M. Vannier
Bruce A. Hanna
New York University School of Medicine, Bellevue Hospital, and
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Adeleh Ebrahimzadeh
The City of New York Department of Health, New York, New York;
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L. Bruce Elliott
Texas Department of Health, Austin, Texas;
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Margie A. Morgan
Cedars-Sinai Medical Center, Los Angeles, and
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Susan M. Novak
Kaiser Permanente, North Hollywood, California; and
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Sabine Rusch-Gerdes
Forschungszentrum Borstel, Borstel, Germany
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Millie Acio
Cedars-Sinai Medical Center, Los Angeles, and
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Denise F. Dunbar
Texas Department of Health, Austin, Texas;
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T. Michele Holmes
Texas Department of Health, Austin, Texas;
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Charles H. Rexer
New York University School of Medicine, Bellevue Hospital, and
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Chiminyan Savthyakumar
The City of New York Department of Health, New York, New York;
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Ann M. Vannier
Kaiser Permanente, North Hollywood, California; and
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DOI: 10.1128/JCM.37.3.748-752.1999
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Tables

  • Table 1.

    Specimen types and recovery of mycobacteria

    Specimen typeNo. of specimensNo. of positive specimens (%)
    Respiratory2,210321 (14.5)
    Body fluids4644 (0.9)
    Tissue39523 (5.8)
    Stool613 (4.9)
    Urine1670 
    Bone marrow182 (11.1)
    Othera150 
    Total3,330353 (10.6)
    • ↵a Specimen source not specified.

  • Table 2.

    Distribution of isolates recovered in each culture system and in combinations of solid and liquid systems

    OrganismTotal no. (%) of isolates recovered witha:
    Total, all media960 + solid media460 TB + solid mediaTotal, 960Total, 460 TBTotal, solid media960 only460 TB onlySolid media only
    M. tuberculosiscomplex132 (100)121 (92)128 (97)102 (77)119 (90)105 (79)4 (3)11 (8)3 (2)
    MAC172 (100)160 (93)136 (79)147 (86)123 (72)106 (62)36 (21)12 (5)3 (2)
    MOTT58 (100)54 (93)44 (76)40 (69)29 (50)39 (67)14 (24)4 (7)10 (17)
    All362 (100)335 (93)308 (85)289 (80)271 (75)250 (69)54 (15)27 (7)16 (4)
    • ↵a 960, BACTEC MGIT 960 system; 460 TB, BACTEC 460 TB system.

  • Table 3.

    Distribution of isolates recovered in culture system and combinations with contaminated cohorts removed from analysis

    OrganismTotal no. of isolates, all mediaTotal no. (%) of isolates recovered witha:
    960 + solid media460 TB + solid mediaTotal, 960Total, 460 TBTotal, solid media
    M. tuberculosiscomplex118111 (94)114 (97)101 (86)108 (92)95 (81)
    MAC160153 (96)127 (79)143 (89)115 (72)102 (64)
    MOTT5349 (92)41 (77)37 (70)28 (53)36 (68)
    All331313 (95)282 (85)281 (85)251 (76)233 (70)
    • ↵a 960, BACTEC MGIT 960 system; 460 TB, BACTEC 460 TB system.

  • Table 4.

    TTD of M. tuberculosis, MAC, and all mycobacteria in BACTEC MGIT 960 and BACTEC 460 TB systems

    Culture systemTTD, days (total no. of isolates detected) for:
    M. tuberculosiscomplexMACAll mycobacteria
    NonpairedaPairedb, cNonpairedaPairedb, dNonpairedaPairedb
    BACTEC 96014.4 (102)14.4 (92)10.0 (147)9.8 (101)11.8 (289)11.1 (213)
    BACTEC 46015.2 (119)13.2 (92)10.4 (123)8.4 (101)12.5 (271)11.3 (213)
    • ↵a Total for isolates recovered with either BACTEC MGIT 960 or BACTEC 460 TB system.

    • ↵b Total for isolates recovered with both systems.

    • c P = 0.055, not significant.

    • d P = 0.001.

  • Table 5.

    TTD of M. tuberculosis for AFB smear-positive and smear-negative isolates in BACTEC 960 and 460 TB systems

    Culture systemTTD (range), no. of isolates
    AFB smear +AFB smear −
    BACTEC 960, total (n = 102)10.6 (5–24), 5018.1 (7–43), 52
    BACTEC 460, total (n = 119)10.8 (3–29), 5118.4 (6–57), 68
    BACTEC 960 (paired)10.8, 4718.1, 45
    BACTEC 460 (paired)10.3, 4716.3, 45
  • Table 6.

    Breakthrough contamination rates

    System% of isolates showing breakthrough contamination
    All sites (n = 3,330)Site A (n = 558)Site B (n = 494)Site C (n = 679)Site D (n = 628)Site E (n = 323)Site F (n = 648)
    BACTEC MGIT 9608.19.35.410.014.61.84.0
    BACTEC 460 TB4.95.00.69.27.10.93.7
    Combined solid media21.127.40.813.231.05.237.8
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Multicenter Evaluation of the BACTEC MGIT 960 System for Recovery of Mycobacteria
Bruce A. Hanna, Adeleh Ebrahimzadeh, L. Bruce Elliott, Margie A. Morgan, Susan M. Novak, Sabine Rusch-Gerdes, Millie Acio, Denise F. Dunbar, T. Michele Holmes, Charles H. Rexer, Chiminyan Savthyakumar, Ann M. Vannier
Journal of Clinical Microbiology Mar 1999, 37 (3) 748-752; DOI: 10.1128/JCM.37.3.748-752.1999

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Multicenter Evaluation of the BACTEC MGIT 960 System for Recovery of Mycobacteria
Bruce A. Hanna, Adeleh Ebrahimzadeh, L. Bruce Elliott, Margie A. Morgan, Susan M. Novak, Sabine Rusch-Gerdes, Millie Acio, Denise F. Dunbar, T. Michele Holmes, Charles H. Rexer, Chiminyan Savthyakumar, Ann M. Vannier
Journal of Clinical Microbiology Mar 1999, 37 (3) 748-752; DOI: 10.1128/JCM.37.3.748-752.1999
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KEYWORDS

bacteriological techniques
Mycobacterium avium complex
Mycobacterium avium-intracellulare Infection
Mycobacterium tuberculosis
tuberculosis

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