Article Figures & Data
Tables
- Table 1.
Investigated strains
Organism Strain designation or origina B. vietnamiensis LMG 10929T, LMG 6998, LMG 6999, CF isolateb B. cepacia DSM 50181, ATCC 25416T, LMG 7000, LMG 12614, LMG 12615, LMG 14294, CF isolatesc B. multivorans LMG 14280, LMG 14293, CF isolatesd B. gladioli DSM 4285, CF isolatese B. mallei ATCC 23344T, ATCC 15310, ATCC 10399 B. pseudomallei ATCC 23343T, ATCC 15682, NCTC 1691, clinical isolatesf R. pickettii ATCC 27511T, clinical isolateg ↵a DSM, Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Braunschweig, Federal Republic of Germany; LMG, Laboratorium voor Microbiologie, Universiteit Gent, Gent, Belgium; ATCC, American Type Culture Collection, Rockville, Md.; NCTC, National Collection of Type Cultures, Central Public Health Laboratory, London, England.
↵b Strain from The Netherlands (n = 1).
↵c Strains from Germany (n = 34), The Netherlands (n = 2), and the United Kingdom (n = 6).
↵d Strains from Germany (n = 60), The Netherlands (n = 11), and Denmark (n = 8).
↵e Strains from Germany (n = 2) and Canada (n = 3).
↵f Strains from Germany (n = 1) and Switzerland (n = 1).
↵g Strain from Germany (n = 1).
- Table 2.
Primers selected for PCR
Target species Primer designationa Sequenceb Annealing temp (°C) Eubacteria Eub-16-1 5′-AGR GTT YGA TYM TGG CTC AG-3′ 56 Eubacteria Eub-16-2 5′-ACC GCG GCT GCT GGC AC-3′ 60 Burkholderia spp. and Ralstoniaspp. BuRa-16-1 5′-AGC ACT TTT GTC CGG RAA-3′ 52 Burkholderia spp. and Ralstoniaspp. BuRa-16-2 5′-AAT CCC CAA CAA CTA GTT G-3′ 54 B. cepacia, B. multivorans, and B. vietnamiensis CeMuVi-16-2457 5′-CCG RCT GTA TTA GAG CCA-3′ 55 B. gladioli Gl-16-2457 5′-CGA AGG ATA TTA GCC CTC-3′ 54 B. cepacia Ce-16-21028 5′-AGC ACT CCC RCC TCT CAG-3′c 59 B. multivorans andB. vietnamiensis MuVi-16-21028 5′-AGC ACT CCC GAA TCT CTT-3′ 54 B. mallei and B. pseudomallei MaPs-16-2457 5′-CTC CGG GTA TTA GCC AGA-3′ 56 Ralstonia pickettii Pi-16-2461 5′-TCG ACC CCA GGT ATT AAC-3′ 54 B. vietnamiensis, B. mallei, and B. pseudomallei ViMaPs-23-1 5′-CCT TTG GGT CAT CCT GGA-3′ 56 B. cepacia and B. vietnamiensis CeVi-23-2 5′-TCC TAC CAT GCG TGC AA-3′ 52 B. mallei Ma-23-2 5′-CAC CGA AAC TAG CA-3′ 42 - Table 3.
Specificity of primer combinations for the identification of Burkholderia spp.
Primer designationa Target organisms Size of PCR product (bp) for: B. cepaciagenomovar B. multivorans B. vietnamiensis B. gladioli B. mallei B. pseudomallei R. pickettii I III IV Eub-16-1 + Eub-16-2 Eubacteria 519 519 519 519 519 519 519 519 519 BuRa-16-1 + BuRa-16-2 Burkholderia andRalstoniaspp. 409 409 409 409 409 409 409 409 409 Eub-16-1 + CeMuVi-16-2457 B. cepacia,B. multivorans, and B. vietnamiensis 463 463 463 463 463 Eub-16-1 + Ce-16-21028 B. cepacia 1,015 1,015 1,015 Eub-16-1 + MuVi-16-21028 B. multivorans and B. vietnamiensis 1,015 1,015 ViMaPs-23-1 + CeVi-23-2 B. vietnamiensis 1,055 Eub-16-1 + Gl-16-2457 B. gladioli 463 Eub-16-1 + MaPs-16-2457 B. mallei and B. pseudomallei 463 463 ViMaPs-23-1 + Ma-23-2 B. mallei 526 Eub-16-1 + Pi-16-2461 R. pickettii 467 ↵a For sequences see Table 2.