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Journal of Clinical Microbiology
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Bacteriology

Distribution of Porphyromonas gingivalisStrains with fimA Genotypes in Periodontitis Patients

Atsuo Amano, Ichiro Nakagawa, Kosuke Kataoka, Ichijiro Morisaki, Shigeyuki Hamada
Atsuo Amano
Division of Special Care Dentistry and
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Ichiro Nakagawa
Departments of Oral Microbiology and
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Kosuke Kataoka
Preventive Dentistry, Osaka University Faculty of Dentistry, Suita-Osaka 565-0871, Japan
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Ichijiro Morisaki
Division of Special Care Dentistry and
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Shigeyuki Hamada
Departments of Oral Microbiology and
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DOI: 10.1128/JCM.37.5.1426-1430.1999
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ABSTRACT

Fimbriae (FimA) of Porphyromonas gingivalis are filamentous components on the cell surface and are thought to play an important role in the colonization and invasion of periodontal tissues. We previously demonstrated that fimA can be classified into four variants (types I to IV) on the basis of the nucleotide sequences of the fimA gene. In the present study, we attempted to detect the four different fimA genes in saliva and plaque samples isolated from patients with periodontitis using the PCR method. Four sets of fimA type-specific primers were designed for the PCR assay. These primers selectively amplified 392-bp (type I), 257-bp (type II), 247-bp (type III), and 251-bp (type IV) DNA fragments of the fimA gene. Positive PCR results were observed with reference strains of P. gingivalis in a type-specific manner. All other laboratory strains of oral and nonoral bacteria gave negative results. The sensitivity of the PCR assay for fimAtype-specific detection was between 5 and 50 cells of P. gingivalis. Clinical samples were obtained from saliva and subgingival plaque from deep pockets (≥4 mm) of 93 patients with periodontitis. Bacterial genomic DNA was isolated from the samples, and the targeted fragments were amplified by PCR. The presence of P. gingivalis was demonstrated in 73 patients (78.5%), and a singlefimA gene was detected in most patients. The distribution of the four fimA types among the P. gingivalis-positive patients was as follows: type I, 5.4%; type II, 58.9%; type III, 6.8%; type IV, 12.3%; types I and II, 6.8%; types II and IV, 2.7%; and untypeable, 6.8%. P. gingivalis with type II fimA was detected more frequently in the deeper pockets, and a significant difference of the occurrence was observed between shallow (4 mm) and deep (≥8 mm) pockets. These results suggest that P. gingivalis strains that possess type II fimA are significantly more predominant in periodontitis patients, and we speculate that these organisms are involved in the destructive progression of periodontal diseases.

  • Copyright © 1999 American Society for Microbiology
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Distribution of Porphyromonas gingivalisStrains with fimA Genotypes in Periodontitis Patients
Atsuo Amano, Ichiro Nakagawa, Kosuke Kataoka, Ichijiro Morisaki, Shigeyuki Hamada
Journal of Clinical Microbiology May 1999, 37 (5) 1426-1430; DOI: 10.1128/JCM.37.5.1426-1430.1999

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Distribution of Porphyromonas gingivalisStrains with fimA Genotypes in Periodontitis Patients
Atsuo Amano, Ichiro Nakagawa, Kosuke Kataoka, Ichijiro Morisaki, Shigeyuki Hamada
Journal of Clinical Microbiology May 1999, 37 (5) 1426-1430; DOI: 10.1128/JCM.37.5.1426-1430.1999
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KEYWORDS

Bacterial Proteins
Fimbriae Proteins
Periodontitis
Porphyromonas gingivalis

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