Article Figures & Data
Figures
- Fig. 1.
Comparison of the sensitivities of the two RT-PCR methods by gel electrophoresis (A) and Southern blotting (B) with serial dilutions of RNA from culture of B. burgdorferi sensu stricto PBre. Lanes: 1 to 6, one-step RT-PCR protocol; 8 to 13, two-step RT-PCR protocol; 1 and 8, sterile water (negative controls); 2 and 9, 10−4-fold dilution; 3 and 10, 10−3-fold dilution; 4 and 11, 10−2-fold dilution; 5 and 12, 10-fold dilution; 6 and 13, no dilution; 7, 100-bp DNA ladder (Gibco-BRL, Cergy-Pontoise, France).
- Fig. 2.
Gel electrophoresis (A) and Southern blot (B) analysis of one-step RT-PCR products from synovial and heart samples from four mice infected with B. burgdorferi sensu stricto PBre. Lanes: 1, sterile water (negative control); 2, 4, 6, and 8, synovial samples from infected mice; 3, 5, 7, and 9, heart samples from the corresponding infected mice. 10, synovial sample from a control mouse (uninfected); 11, 30 fg of RNA of B. burgdorferi PBre; 12, 100-bp DNA ladder (Gibco-BRL, Cergy-Pontoise, France).
