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Journal of Clinical Microbiology
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Bacteriology

One-Step Reverse Transcriptase PCR Method for Detection of Borrelia burgdorferi mRNA in Mouse Lyme Arthritis Tissue Samples

F. X. Limbach, B. Jaulhac, Y. Piémont, J. L. Kuntz, H. Monteil, J. Sibilia
F. X. Limbach
Institut de Bactériologie de la Faculté de Médecine, Université Louis Pasteur et Hôpitaux Universitaires de Strasbourg,and
Service de Rhumatologie, Hôpitaux Universitaires de Strasbourg, 67000 Strasbourg, France
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B. Jaulhac
Institut de Bactériologie de la Faculté de Médecine, Université Louis Pasteur et Hôpitaux Universitaires de Strasbourg,and
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Y. Piémont
Institut de Bactériologie de la Faculté de Médecine, Université Louis Pasteur et Hôpitaux Universitaires de Strasbourg,and
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J. L. Kuntz
Service de Rhumatologie, Hôpitaux Universitaires de Strasbourg, 67000 Strasbourg, France
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H. Monteil
Institut de Bactériologie de la Faculté de Médecine, Université Louis Pasteur et Hôpitaux Universitaires de Strasbourg,and
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J. Sibilia
Service de Rhumatologie, Hôpitaux Universitaires de Strasbourg, 67000 Strasbourg, France
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DOI: 10.1128/JCM.37.6.2037-2039.1999
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    Fig. 1.

    Comparison of the sensitivities of the two RT-PCR methods by gel electrophoresis (A) and Southern blotting (B) with serial dilutions of RNA from culture of B. burgdorferi sensu stricto PBre. Lanes: 1 to 6, one-step RT-PCR protocol; 8 to 13, two-step RT-PCR protocol; 1 and 8, sterile water (negative controls); 2 and 9, 10−4-fold dilution; 3 and 10, 10−3-fold dilution; 4 and 11, 10−2-fold dilution; 5 and 12, 10-fold dilution; 6 and 13, no dilution; 7, 100-bp DNA ladder (Gibco-BRL, Cergy-Pontoise, France).

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    Fig. 2.

    Gel electrophoresis (A) and Southern blot (B) analysis of one-step RT-PCR products from synovial and heart samples from four mice infected with B. burgdorferi sensu stricto PBre. Lanes: 1, sterile water (negative control); 2, 4, 6, and 8, synovial samples from infected mice; 3, 5, 7, and 9, heart samples from the corresponding infected mice. 10, synovial sample from a control mouse (uninfected); 11, 30 fg of RNA of B. burgdorferi PBre; 12, 100-bp DNA ladder (Gibco-BRL, Cergy-Pontoise, France).

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One-Step Reverse Transcriptase PCR Method for Detection of Borrelia burgdorferi mRNA in Mouse Lyme Arthritis Tissue Samples
F. X. Limbach, B. Jaulhac, Y. Piémont, J. L. Kuntz, H. Monteil, J. Sibilia
Journal of Clinical Microbiology Jun 1999, 37 (6) 2037-2039; DOI: 10.1128/JCM.37.6.2037-2039.1999

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One-Step Reverse Transcriptase PCR Method for Detection of Borrelia burgdorferi mRNA in Mouse Lyme Arthritis Tissue Samples
F. X. Limbach, B. Jaulhac, Y. Piémont, J. L. Kuntz, H. Monteil, J. Sibilia
Journal of Clinical Microbiology Jun 1999, 37 (6) 2037-2039; DOI: 10.1128/JCM.37.6.2037-2039.1999
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KEYWORDS

Arthritis, Infectious
Borrelia burgdorferi Group
Lyme disease
RNA, Messenger

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