Article Figures & Data
Figures
- Fig. 1.
RAPD patterns of the 19 isolates of Bacillusspecies generated by APPCR with the primers OPA-1 (A) and ERIC1 (B). Lanes: M, molecular size marker; 1 to 13, B. cereus isolates P2 to P5, P8, P9, P12 to P15, S1, N1, and L1, respectively; 14 to 16,B. sphaericus isolates P1, L11, and L12, respectively; 17 to 19, isolates U1 to U3; 20 to 28, nine additional epidemiologically unrelated B. cereus isolates (see Table 1).
Tables
- Table 1.
Phenotypic and genotypic characteristics of 50 isolates of Bacillus species
Isolate (location)a Species Date of isolation (day/mo/yr) Source Antibiotype as determined by: Biotype as determined by Vitek Bacilluscard RAPD pattern as determined with OPA-1 or ERIC1 primer Clone (B. cereus) Disk diffusion E test Isolates from patients P1 (OW) B. sphaericus 1/5/1990 Blood 2 III C f P2 (OW) B. cereus 20/9/1990 Pleural effusion 1 I A a A P3 (SICU) B. cereus 10/9/1990 Blood 1 II B b B P4 (ED) B. cereus 19/9/1990 Blood 1 II B b B P5 (ED) B. cereus 5/10/1990 Blood 1 I A a A P6 (OW) B. cereus 20/9/1990 Blood 1 II B b B P7 (PW) B. cereus 20/9/1990 Blood 1 II B b B P8 (ED) B. cereus 20/9/1990 Blood 1 II B b B P9 (ED) B. cereus 20/9/1990 Blood 1 II B b B P10 (ED) B. cereus 20/9/1990 Blood 1 II B b B P11 (ED) B. cereus 20/9/1990 Blood 1 II B b B P12 (ED) B. cereus 4/10/1990 Blood 1 II B b B P13 (OW) B. cereus 4/10/1990 Blood 1 II B b B P14 (ED) B. cereus 5/10/1990 Blood 1 II B b B P15 (ED) B. cereus 5/10/1990 Blood 1 II B b B P16 (ED) B. cereus 5/10/1990 Blood 1 II B b B Isolates from alcohol used in hospital S1–S9 (ED) B. cereus 8/10/1990 70% alcohol 1 II B b B S10–S14 (OW) B. cereus 8/10/1990 70% alcohol 1 II B b B N1–N5 (PD) B. cereus 12/10/1990 95% alcohol 1 II B b B Isolates from factory L1–L10 B. cereus 23/10/1990 95% alcohol 1 II B b B L11–12 B. sphaericus 23/10/1990 95% alcohol 2 IV C g Epidemiologically unrelated isolates U-1 B. cereus 9/3/1998 Blood 1 V B c C U-2 B. cereus 13/8/1998 Blood 1 VI A d D U-3 B. cereus 15/8/1998 Blood 1 VII B e E ↵a OW, oncology ward; SICU, surgical intensive care unit; ED, emergency department; PW, pediatric ward; PD, pharmacy department.
- Table 2.
Susceptibilities to 16 antimicrobial agents of 50 isolates of Bacillus species identified by antibiotype
Antimicrobial agent MIC (μg/ml) forBacillus sp. E-test antibiotype: Result forBacillus sp. disk antibiotypea: I II III IV V VI VII 1 2 Penicillin 16 64 16 4 4 ≥256 4 R R Ampicillin ≥256 ≥256 8 2 8 ≥256 64 R S Ampicillin-sulbactam 4 32 4 1 4 32 4 Cefazolin 64 ≥256 8 2 64 ≥256 128 R S Cefotaxime 128 ≥256 4 0.5 32 ≥256 32 Cefepime ≥256 64 4 0.25 ≥256 ≥256 ≥256 Imipenem 0.25 0.06 0.5 0.06 1 2 4 Chloramphenicol 1.0 0.5 0.5 0.5 4 0.25 0.25 S S Erythromycin 0.03 0.03 0.03 0.06 0.25 0.12 0.5 S S Gentamicin 1 2 1 1 2 2 1 S S Trimethoprim-sulfamethoxazole ≥32 ≥32 ≥32 ≥32 ≥32 ≥32 ≥32 Vancomycin 2 2 2 1 4 4 4 S S Teicoplanin 4 2 2 1 2 2 2 Ciprofloxacin 1.0 1.0 1.0 0.5 4 8 4 Minocycline S S Tetracycline S S ↵a R, resistant; S, susceptible.
