Differentiation among Members of the Mycobacterium tuberculosis Complex by Molecular and Biochemical Features: Evidence for Two Pyrazinamide-Susceptible Subtypes ofM. bovis

DOI: 10.1128/JCM.38.1.152-157.2000

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Fig. 1.
Spoligotypes and spoligotype groups among the M. tuberculosis complex isolates analyzed.

Table 1.

Biochemical and genetical characteristics of M. tuberculosis H37Rv and the strains analyzed^a

Organism and group	PZA sensitivity	Niacin accumulation	Nitrate reduction	Growth in presence of TCH	Change of color of bromcresol medium	Colony morphology (growth)	Growth on Lebek medium	Presence of mtb40	Presence of pncA mutation (C to G)	Presence of oxyR mutation (G to A)
M. tuberculosis H37Rv (n = 1)	S	+	+	+	+	Eugonic	Aerophilic	+	−	−
M. bovis
Group 1 (n = 35)	R (100)^b	− (100)	− (100)	− (100)	− (100)	Dysgonic (100)	Microaerophilic (100)	− (100)^c	+ (100)^d	+ (100)^d
Group 2 (n = 15)	S (100)	− (100)	− (100)	− (94), + (6)^e	− (100)	Dysognic (100)	Microaerophilic (100)	− (100)	− (100)^d	+ (100)^d
Group 3 (n = 2)	S (100)	− (100)	− (100)	− (100)	− (100)	Dysognic (100)	Microaerophilic (100)	− (100)	− (100)	+ (100)
M. africanum
Group 4 (n = 20)	S (100)	+ (55), − (45)	+ (65), − (35)	− (85), + (15)^e	− (100)	Dysognic (100)	Microaerophilic (100)	+ (70), − (30)	− (100)^d	− (100)^d
Group 5 (n = 24)	S (100)	+ (30), − (70)	+ (100)	+ (100)	− (100)	Dysognic (100)	Microaerophilic (100)	+ (100)	− (100)^d	− (100)^d

↵a Abbreviations and symbols: S, susceptible, R, resistant; +, positive test result; −, negative test result.
↵b Values in parentheses are percentage of isolates.
↵c Ten randomly chosen strains were tested.
↵d Five randomly chosen strains were tested.
↵e Strains were resistant to isoniazid and cross-resistant to TCH.