A Clonal Subpopulation of Leptospira interrogans Sensu Stricto Is the Major Cause of Leptospirosis Outbreaks in Brazil

AP-PCR fingerprints of genomic DNAs of theLeptospira isolates described in Table 1 and Table 2. An autoradiograph of a 4% acrylamide–50% urea gel is shown. (A) Lane 1, serovar copenhageni strain Winjberg; lanes 2 to 33, SP-03, SP-04, SP-10, SP-22, SP-25, SP-42, SP-44, SP-53, SP-58, SP-59, SP-67, SP-69, SP-73, SP-80, SP-104, SP-13, SP-14, SP-18, SP-20, SP-28, SP-36, SP-49, SP-50, SP-61, SP-70, SP-102, SP-149, SP-150, SP-163, SP-167, SP-212, and SP-217, respectively; lane M, marker. (B) Lanes 1 and 16, serovar copenhageni strain Winjberg; lanes 2 to 13; SP-74, SP-75, SP-86, SP-17, SP-52, SP-68, SP-69, SP-103, SP-151, SP-204, 1010, and 1015, respectively; lanes 14 and 15, serovar canicola strains chiffon and Hond Utrecht, respectively. The arrows correspond to major differences between the profiles of serovars canicola and copenhageni. (C) Lane 1, SP-58; lane 2, serovar grippotyphosa strain Moskva V.