Article Figures & Data
Figures
- Fig. 1.
Results of agarose gel electrophoresis demonstrating the two different fragment sizes obtained with PCR primers STMA17 and STMA18. Lane 1, amplified product of B. pertussis patient isolate KP92\2\92; lane 2, B. pertussis ATCC 9797; lane 3,B. parapertussis ATCC 15311; lane 4, B. bronchiseptica ATCC 786; lane 5, B. bronchiseptica19395; lane M, a 50-bp ladder.
- Fig. 2.
The five different sequences encompassing the PT promoter region (the 239 or 249 nucleotides upstream of the start codon of the PT subunit 1 gene amplified with flanking primers) found among the 33 ATCC reference strains and patient isolates sequenced. The three restriction sites used for species identification are marked with boxes. Restriction enzyme recognition sequences are underlined. The nucleotide positions in sequence b (B. pertussis ATCC 9797 and ATCC 9340) that differ from those in sequence a (B. pertussis patient isolates) are marked in boldface. The nucleotide positions in sequence e (B. bronchiseptica ATCC 19395) that differ from those in sequence d (B. bronchiseptica ATCC 786 and AB 1254) are also marked in boldface. Sequence c is the sequence common to all of the B. parapertussis strains.
- Fig. 3.
The four different restriction patterns resulting from the five different sequence types in Fig. 2 obtained with restriction enzymes TaqI, BglI, and HaeII.
- Fig. 4.
Results from colorimetric restriction enzyme analysis ofBordetella strains representing the five different PT promoter sequence types in Fig. 2 to confirm the four restriction patterns in Fig. 3. The negative control corresponds to a PCR-negative sample that was immobilized onto magnetic beads and mixed with fusion protein and substrate (see text for details).
Tables
- Table 1.
Strains used in the study
Strain or patient isolate Sequence type Restriction pattern Source Year of isolation B. pertussis KP92\2\92 a I PVTa92-95 1992 KP92\9\92 a I PVT 92-95 1992 KP92\12\92 a I PVT 92-95 1992 KP92\42\92 a I PVT 92-95 1992 KP92\47\92 a I PVT 92-95 1992 KP92\357\93 a I PVT 92-95 1993 KP92\757\94 a I PVT 92-95 1994 KP92\1145\94 a I PVT 92-95 1994 KP92\1162\94 a I PVT 92-95 1994 R613257 SBL 77-09-29 a I Routine clinical sample 1977 RA776 SBL 75-10-02 a I Routine clinical sample 1975 ATCC 9340 b I ATCC ATCC 9797 b I ATCC B. parapertussis KP92\11\92 c II PVT 92-95 1992 KP92\41\92 c II PVT 92-95 1992 KP92\83\93 c II PVT 92-95 1993 KP92\106\93 c II PVT 92-95 1993 KP86\148\86 c II PVT 86-87 1986 KP92\214\93 c II PVT 92-95 1993 KP92\218\93 c II PVT 92-95 1993 KP86\250\86 c II PVT 86-87 1986 KP92\283\93 c II PVT 92-95 1993 KP92\308\93 c II PVT 92-95 1993 KP92\373\93 c II PVT 92-95 1993 KP92\377\93 c II PVT 92-95 1993 KP92\437\93 c II PVT 92-95 1993 KP92\756\94 c II PVT 92-95 1994 KP92\1180\94 c II PVT 92-95 1994 ATCC 15237 c II ATCC ATCC 15311 c II ATCC B. bronchiseptica ATCC 786 d III ATCC AB 1254 d III SSb ATCC 19395 e IV ATCC
