Article Figures & Data
Tables
- Table 1.
Comparison of DNA extraction kit sensitivity from spiked specimens as determined by mean time-resolved fluorescence valuesa
Specimen (concn [PFU/ml]) NS PG QIA MP IQ GCC Mean value No. posb Mean value No. posb Mean value No. posb Mean value No. posb Mean value No. posb Mean value No. posb BAL (4) 293,768 6/6 242,640 6/6 123,226 6/6 375,405 5/6 97,325 4/6 14,300 2/6 BLD (4) 292,375 6/6 79,808 5/6 587,679 6/6 42,176 4/6 63,402 4/6 220,722 6/6 Plasma (4) 359,998 6/6 296,304 6/6 123,173 6/6 303,136 6/6 47,882 2/6 8,894 1/6 CSF 4 662,433 6/6 527,460 5/6 65,469 3/6 97,573 2/6 31,787 2/6 1,486 0/6 2 72,042 5/6 64,069 4/6 0.8 35,542 5/6 25,847 3/6 0.4 28,943 3/6 8,193 1/6 - Table 2.
Comparison of the NucliSens isolation kit sensitivity to other extraction methods from spiked specimens as determined by mean time-resolved fluorescence valuesa
Specimen (concn [PFU/ml]) Mean time-resolved fluorescence values (P value) for paired tests: NS/PG NS/QIA NS/MP NS/IQ NS/GCC BAL (4) 293,768/242,640 (0.3807b) 293,768/123,226 (0.0255) 293,768/375,405 (0.3637b) 293,768/97,325 (0.0654b) 293,768/14,300 (0.0009) BLD (4) 292,375/79,808 (0.0265) 292,375/587,679 (0.5139b) 292,375/42,176 (0.0235) 292,375/63,402 (0.0087) 292,375/220,722 (0.4887b) Plasma (4) 359,998/296,304 (0.6317b) 359,998/123,173 (0.0105) 359,998/303,136 (0.3558b) 359,998/47,882 (0.0022) 359,998/8,894 (0.0027) CSF 4 662,433/527,460 (0.4165b) 662,433/65,469 (0.0121) 662,433/97,573 (0.0416) 662,433/31,787 (0.0085) 662,433/1,486 (0.0076) 2 72,042/64,069 (0.7741b) 0.8 35,542/25,847 (0.4816b) 0.4 28,943/8,193 (0.1353b) - Table 3.
Comparison of cost, processing time, and other miscellaneous aspects between the evaluated DNA extraction kits
Extraction method Cost/testa Processing timeb (h:min) Maximum sample vol (μl) Recovered extraction vol (μl) Required reagents not included in kit Special equipmente Hands-onc Totald NSf $4.00 02:28 03:08 200g 50 70% ethanol Vacuum aspirator Acetone PG 0.23 01:49 04:39 100 20 Proteinase K 100% isopropanol 70% ethanol Glycogen QIA 1.10 01:35 01:55 200 200 100% ethanol MP 0.69 01:04 01:59 150 (BAL, CSF) 35 100% isopropanol Refrigerated centrifuge 50 (PLM) 75% ethanol 12.5 (BLD) IQ 0.84 01:53 02:38 100 100 100% isopropanol 70% ethanol GCC 1.08 00:45 00:55 200 200 Fitted heat blockh ↵a Based on pricing quote (early 1999) from each manufacturer for large-volume orders.
↵b Determined for an 18-sample run. Timing began with aliquoting of first reagent or sample and concluded with recovery of extracted DNA.
↵c Time required to perform manual manipulation of tubes, reagents, and samples.
↵d Time required to obtain extracted DNA, including hands-on time and incubation periods.
↵e Excluding standard laboratory equipment.
↵f An automated extractor is available for laboratories with high sample volumes.
↵g Kit available for larger-sample-volume processing.
↵h Bore size must be large enough to completely contain capture column. Manufacturer recommends specific models.