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Journal of Clinical Microbiology
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Bacteriology

Correlation of Oxacillin MIC with mecAGene Carriage in Coagulase-Negative Staphylococci

Zafar Hussain, Luba Stoakes, Viki Massey, Deb Diagre, Viivi Fitzgerald, Sameer El Sayed, Robert Lannigan
Zafar Hussain
London Health Sciences Centre 1 and Department of Microbiology and Immunology, The University of Western Ontario, 2 London, Ontario, Canada
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Luba Stoakes
London Health Sciences Centre 1 and
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Viki Massey
London Health Sciences Centre 1 and
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Deb Diagre
London Health Sciences Centre 1 and
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Viivi Fitzgerald
London Health Sciences Centre 1 and
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Sameer El Sayed
London Health Sciences Centre 1 and Department of Microbiology and Immunology, The University of Western Ontario, 2 London, Ontario, Canada
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Robert Lannigan
London Health Sciences Centre 1 and Department of Microbiology and Immunology, The University of Western Ontario, 2 London, Ontario, Canada
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ABSTRACT

The National Committee for Clinical Laboratory Standards has recently changed the oxacillin breakpoint from ≥4 mg/liter to ≥0.5 mg/liter to detect methicillin-resistant coagulase-negative staphylococci (CoNS) because the previous breakpoint lacked sensitivity. To determine the correlation between the new oxacillin breakpoint and the presence of themecA gene, 493 CoNS of 11 species were tested. The presence of the mecA gene was determined by PCR, and oxacillin susceptibility was determined by the agar dilution method with Mueller-Hinton agar containing 2% NaCl and oxacillin (0.125 to 4.0 mg/liter). The new breakpoint correctly classified all CoNS strains with mecA as methicillin resistant and strains ofStaphylococcus epidermidis, S. haemolyticus, and S. hominiswithout mecA as methicillin susceptible. The breakpoint of ≥0.5 mg/liter was not specific for S. cohnii, S. lugdunensis, S. saprophyticus, S. warneri, and S. xylosus, in that it categorized 70 of 74 strains of these species withoutmecA (94.6%) as methicillin resistant. The results of this study indicate that the new oxacillin breakpoint accurately identifies strains of CoNS with mecAbut is not specific for strains of certain species of CoNS withoutmecA.

  • Copyright © 2000 American Society for Microbiology
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Correlation of Oxacillin MIC with mecAGene Carriage in Coagulase-Negative Staphylococci
Zafar Hussain, Luba Stoakes, Viki Massey, Deb Diagre, Viivi Fitzgerald, Sameer El Sayed, Robert Lannigan
Journal of Clinical Microbiology Feb 2000, 38 (2) 752-754; DOI:

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Correlation of Oxacillin MIC with mecAGene Carriage in Coagulase-Negative Staphylococci
Zafar Hussain, Luba Stoakes, Viki Massey, Deb Diagre, Viivi Fitzgerald, Sameer El Sayed, Robert Lannigan
Journal of Clinical Microbiology Feb 2000, 38 (2) 752-754; DOI:
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