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Journal of Clinical Microbiology
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Bacteriology

Multiplex PCR for Detection of Genes forStaphylococcus aureus Enterotoxins, Exfoliative Toxins, Toxic Shock Syndrome Toxin 1, and Methicillin Resistance

Manisha Mehrotra, Gehua Wang, Wendy M. Johnson
Manisha Mehrotra
Special Project Unit, Bureau of Microbiology, Canadian Science Centre for Human and Animal Health, Winnipeg, Manitoba, Canada
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Gehua Wang
Special Project Unit, Bureau of Microbiology, Canadian Science Centre for Human and Animal Health, Winnipeg, Manitoba, Canada
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Wendy M. Johnson
Special Project Unit, Bureau of Microbiology, Canadian Science Centre for Human and Animal Health, Winnipeg, Manitoba, Canada
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DOI: 10.1128/JCM.38.3.1032-1035.2000
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ABSTRACT

A multiplex PCR assay for detection of genes for staphylococcal enterotoxins A to E (entA, entB,entC, entD, and entE), toxic shock syndrome toxin 1 (tst), exfoliative toxins A and B (etaA and etaB), and intrinsic methicillin resistance (mecA) was developed. Detection offemA was used as an internal positive control. The multiplex PCR assay combined the primers for sea tosee and femA in one set and those foreta, etb, tst, mecA, and femA in the other set. Validation of the assay was performed using 176 human isolates of Staphylococcus aureus. This assay offers a very specific, quick, reliable, and inexpensive alternative to conventional PCR assays used in clinical laboratories to identify various staphylococcal toxin genes.

  • Copyright © 2000 American Society for Microbiology
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Multiplex PCR for Detection of Genes forStaphylococcus aureus Enterotoxins, Exfoliative Toxins, Toxic Shock Syndrome Toxin 1, and Methicillin Resistance
Manisha Mehrotra, Gehua Wang, Wendy M. Johnson
Journal of Clinical Microbiology Mar 2000, 38 (3) 1032-1035; DOI: 10.1128/JCM.38.3.1032-1035.2000

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Multiplex PCR for Detection of Genes forStaphylococcus aureus Enterotoxins, Exfoliative Toxins, Toxic Shock Syndrome Toxin 1, and Methicillin Resistance
Manisha Mehrotra, Gehua Wang, Wendy M. Johnson
Journal of Clinical Microbiology Mar 2000, 38 (3) 1032-1035; DOI: 10.1128/JCM.38.3.1032-1035.2000
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KEYWORDS

Bacterial Toxins
Enterotoxins
Genes, Bacterial
methicillin resistance
polymerase chain reaction
Staphylococcus aureus
Superantigens

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