DOI: 10.1128/JCM.38.4.1545-1551.2000
Article Figures & Data
Figures
- Fig. 1.
Dendrogram based on Bousfield's coefficient values (SO) between the 29 strains of Y. pestis studied and generated by cluster analysis (UPGMA). (a) O, Orientalis; M, Medievalis; A, Antiqua. (b) Ribotypes previously determined by Guiyoule and collaborators (19). ND, not determined.
- Fig. 2.
Comparison of Y. pestis with otherYersinia species by plotting the ratios of 12:0 and 16:0 and 14:0 and 16:0 fatty acids. The ratios for the species other thanY. pestis were obtained from a previous study (26).
Tables
- Table 1.
Characteristics of the 29 strains of Y. pestisused for cellular fatty acid analysis
Strain no. Pasteur Institut no. Geographic origina Year of isolation Host Biotype Ribotypeb 613 548 Burma 1970 UNc Orientalis G 579 195 Bombay 1908 Human Orientalis B 569 EXU 56 Brazil 1967 Rodent Orientalis B 1049 197 Dalat 1965 Human Orientalis G 685 H.10 Hamburg 1952 UN Orientalis B 696 H.26 Hamburg 1952 UN Orientalis B 544 Keny. MMI Kenya UNc Human Antiqua F 552 Margar Kenya UN Human Antiqua M 520 PKR VIII Kurdistan 1947 Rodent Medievalis O 304 6/69+ Madagascar 1969 Human Orientalis B 529 112 Madagascar 1951 Human Orientalis B 1511 197/96 Madagascar 1996 Human Orientalis B 1491 167/96 Madagascar 1996 Human Orientalis B 643 4/83 Madagascar 1983 Human Orientalis Q 649 12/85 Madagascar 1985 Human Orientalis Q 1512 198/96 Madagascar 1996 Rodent Orientalis Q 666 100-92 Madagascar 1992 Human Orientalis R 635 11/85 Madagascar 1985 Human Orientalis R 668 41/93 Madagascar 1993 Human Orientalis R 1484 61/94 Madagascar 1994 Human Orientalis T 1482 57/94 Madagascar 1994 Human Orientalis T 28 EV76 Madagascar 1926 Human Orientalis UN 1537 254 Namibia 1984 Human Orientalis V 940 65-30 Nha Trang 1965 UN Orientalis G 513 64-65 Nha Trang 1964 UN Orientalis G 507 55.720 Saigon 1955 UN Orientalis E 532 55.801 Saigon 1955 UN Orientalis E 524 Fay. Sy Senegal 1944 Human Orientalis D 572 A1122 United States <1942 Human Orientalis B - Table 2.
Cellular fatty acid composition of Yersinia pestis strains: major components
Strain of Y. pestis Ribotype Amountaof indicated compoundb 12:0 3-OH- 12:0 2-OH- 12:0 14:0 a15:1 15:0 3-OH- 14:0 16:1ω9c 16:0 17:0-cyc 17:0 18:2ω9,12 18:1ω9c 18:1ω9t 18:0 19:0-cyc 304 B 1.25 0.19 — 1.32 0.49 0.14 6.40 30.40 35.03 8.61 — 0.78 0.80 11.16 1.47 — 529 B 2.19 — — 1.09 0.39 0.26 11.84 33.49 29.60 7.20 — 0.97 0.84 9.90 1.24 — 569 B 1.18 0.38 — 0.63 0.48 — 7.58 34.42 32.95 5.64 — 0.87 1.04 11.79 1.42 — 572 B 2.09 — — 1.64 0.39 — 10.23 32.94 31.86 8.45 — 0.68 — 10.08 1.01 — 579 B 1.69 — — 1.96 0.51 0.20 7.81 32.19 34.87 9.26 — 1.10 0.81 7.69 0.92 — 685 B 2.35 — — 3.22 0.37 0.14 8.16 39.40 35.23 5.49 — 0.31 0.20 3.38 0.58 — 696 B 3.15 0.08 — 2.10 0.39 0.16 13.08 31.40 33.69 7.03 — 0.75 0.59 4.88 1.81 — 1491 B 1.07 — — 1.38 0.21 0.18 10.12 28.44 35.50 8.23 — 0.76 0.74 10.93 1.76 — 1511 B 1.30 — — 1.65 0.22 0.15 10.63 32.37 34.37 7.00 — 0.56 0.10 9.62 1.62 — 524 D 1.22 — 0.15 0.71 0.45 — 5.67 34.78 31.74 3.60 — 0.91 0.75 17.07 1.86 — 507 E 2.18 — — 1.57 0.47 0.13 8.55 31.31 34.54 8.40 — 0.93 0.90 8.68 1.47 — 532 E 2.96 — — 1.94 0.45 0.17 11.49 40.20 32.62 1.88 — 0.56 — 6.10 0.92 — 544 F 1.23 — — 1.75 0.43 — 8.60 35.62 34.23 8.22 — 0.36 0.54 7.00 0.71 — 513 G 2.74 — 0.09 1.75 0.47 0.16 13.98 35.91 30.29 5.25 — 0.46 — 7.25 0.79 — 613 G 2.70 — — 1.51 0.39 0.10 11.30 36.19 31.02 5.18 — 0.58 0.55 8.71 1.33 — 940 G 1.82 — — 1.62 0.39 0.21 11.30 30.89 33.58 8.26 — 0.64 0.46 8.32 1.24 — 1049 G 1.15 — 0.39 1.07 0.48 0.11 8.45 34.24 32.77 5.47 — 0.89 0.97 11.80 1.43 — 552 M 3.25 — — 1.51 0.59 0.19 10.90 18.24 34.21 17.77 — 0.83 0.68 8.48 1.30 0.44 520 O 1.73 — 0.37 2.51 0.48 0.21 8.47 27.02 37.86 11.07 — 0.65 0.79 7.24 1.20 — 643 Q 2.65 — 0.07 2.00 0.40 0.14 12.57 37.14 28.01 5.21 — 0.60 0.43 8.46 1.08 — 649 Q 2.14 — — 2.29 0.43 0.14 11.37 32.58 36.52 8.06 — 0.66 0.23 3.91 0.95 — 1512 Q 1.07 — — 1.39 0.22 0.21 11.02 30.99 34.08 7.46 — 0.62 0.49 10.19 1.48 — 635 R 1.56 — — 1.40 0.31 0.15 12.48 31.82 33.83 6.75 — 0.54 0.43 9.04 1.15 — 666 R 1.89 — — 1.69 0.29 0.14 11.85 32.81 32.84 6.87 — 0.54 0.44 8.90 1.18 — 668 R 1.04 — — 1.18 0.22 0.15 10.73 29.14 33.33 7.26 0.13 0.89 0.81 12.46 1.73 — 1482 T 1.11 — — 1.38 0.18 0.22 10.72 29.09 33.24 8.01 — 0.27 0.95 10.48 2.79 — 1484 T 1.11 — — 1.38 0.25 0.11 11.74 29.35 34.40 7.99 — 0.80 0.71 9.99 1.29 — 1537 V 1.84 — — 1.59 0.34 0.20 11.17 31.84 32.37 6.93 0.13 0.67 0.55 9.83 1.53 — 28 UNc 2.54 — — 1.80 0.43 — 12.19 30.82 31.93 9.27 — 0.57 0.58 7.96 1.00 — ↵a Values are percentages of total fatty acids and are arithmetic means; —, unintegrated or nonexistent peak.
↵b Number before the colon, number of carbon atoms; number after the colon, number of double bonds; ω9cis, double-bond position from hydrocarbon end of cis isomer; ω9t, double-bond position from hydrocarbon end of trans isomer; cyc, cyclopropane fatty acid; 2-OH or 3-OH, hydroxy group at carbon 2 or 3.
↵c UN, unknown.
- Table 3.
Bousfield's coefficient SO values between Y. pestis strains
Strain SOvaluesa for Y. pestis strain 304 529 569 572 579 685 696 1491 1511 524 507 532 544 513 613 940 1049 552 520 643 649 1512 635 666 668 1482 1484 1537 28 304 1.000 529 0.902 1.000 569 0.936 0.927 1.000 572 0.925 0.958 0.931 1.000 579 0.936 0.911 0.908 0.867 1.000 685 0.857 0.858 0.884 0.874 0.884 1.000 696 0.891 0.919 0.887 0.918 0.917 0.890 1.000 1491 0.953 0.908 0.910 0.932 0.932 0.847 0.902 1.000 1511 0.932 0.943 0.931 0.962 0.946 0.885 0.936 0.947 1.000 524 0.889 0.880 0.934 0.885 0.853 0.831 0.834 0.859 0.878 1.000 507 0.933 0.914 0.894 0.934 0.963 0.863 0.928 0.954 0.951 0.841 1.000 532 0.827 0.886 0.874 0.891 0.867 0.918 0.898 0.836 0.888 0.845 0.863 1.000 544 0.914 0.908 0.925 0.938 0.952 0.918 0.910 0.907 0.940 0.872 0.924 0.901 1.000 513 0.911 0.929 0.930 0.966 0.937 0.883 0.946 0.922 0.971 0.875 0.940 0.909 0.931 1.000 613 0.899 0.950 0.943 0.953 0.886 0.896 0.903 0.863 0.907 0.868 0.889 0.934 0.908 0.929 1.000 940 0.934 0.942 0.913 0.957 0.955 0.875 0.948 0.941 0.960 0.855 0.963 0.890 0.936 0.968 0.912 1.000 1049 0.930 0.933 0.990 0.936 0.914 0.885 0.891 0.915 0.937 0.930 0.900 0.882 0.928 0.937 0.908 0.916 1.000 552 0.827 0.818 0.791 0.834 0.848 0.755 0.832 0.851 0.841 0.735 0.870 0.768 0.814 0.837 0.797 0.863 0.795 1.000 520 0.916 0.847 0.864 0.877 0.917 0.840 0.865 0.905 0.883 0.824 0.918 0.805 0.883 0.877 0.827 0.899 0.861 0.837 1.000 643 0.845 0.937 0.892 0.912 0.875 0.886 0.902 0.853 0.902 0.856 0.874 0.918 0.896 0.930 0.962 0.907 0.899 0.783 0.816 1.000 649 0.895 0.910 0.879 0.930 0.929 0.916 0.947 0.908 0.936 0.823 0.927 0.891 0.927 0.939 0.889 0.942 0.884 0.819 0.890 0.882 1.000 1512 0.938 0.936 0.921 0.946 0.936 0.861 0.927 0.951 0.969 0.863 0.944 0.868 0.919 0.953 0.891 0.964 0.927 0.841 0.877 0.883 0.918 1.000 635 0.921 0.948 0.922 0.950 0.942 0.877 0.953 0.932 0.973 0.866 0.944 0.893 0.927 0.982 0.915 0.969 0.929 0.841 0.880 0.920 0.933 0.963 1.000 666 0.844 0.929 0.892 0.916 0.883 0.889 0.917 0.855 0.905 0.856 0.882 0.923 0.915 0.929 0.957 0.907 0.898 0.785 0.821 0.958 0.894 0.883 0.919 1.000 668 0.934 0.922 0.927 0.929 0.916 0.834 0.910 0.964 0.947 0.883 0.944 0.846 0.895 0.933 0.874 0.939 0.933 0.836 0.873 0.864 0.890 0.951 0.938 0.867 1.000 1482 0.938 0.928 0.919 0.941 0.928 0.839 0.914 0.966 0.951 0.864 0.953 0.853 0.908 0.938 0.877 0.953 0.925 0.848 0.884 0.869 0.901 0.960 0.942 0.868 0.970 1.000 1484 0.945 0.939 0.915 0.943 0.940 0.854 0.927 0.967 0.961 0.856 0.962 0.863 0.921 0.952 0.887 0.966 0.920 0.860 0.895 0.882 0.922 0.970 0.962 0.881 0.959 0.973 1.000 1537 0.930 0.930 0.910 0.948 0.910 0.856 0.923 0.923 0.949 0.897 0.917 0.883 0.896 0.957 0.926 0.941 0.944 0.816 0.847 0.920 0.907 0.941 0.951 0.919 0.942 0.939 0.936 1.000 28 0.913 0.941 0.895 0.956 0.949 0.863 0.946 0.917 0.937 0.849 0.954 0.892 0.918 0.960 0.917 0.970 0.901 0.857 0.895 0.918 0.931 0.938 0.956 0.923 0.919 0.931 0.945 0.937 1.000 ↵a The values were calculated by the method of Bousfield (7).
