Skip to main content
  • ASM
    • Antimicrobial Agents and Chemotherapy
    • Applied and Environmental Microbiology
    • Clinical Microbiology Reviews
    • Clinical and Vaccine Immunology
    • EcoSal Plus
    • Eukaryotic Cell
    • Infection and Immunity
    • Journal of Bacteriology
    • Journal of Clinical Microbiology
    • Journal of Microbiology & Biology Education
    • Journal of Virology
    • mBio
    • Microbiology and Molecular Biology Reviews
    • Microbiology Resource Announcements
    • Microbiology Spectrum
    • Molecular and Cellular Biology
    • mSphere
    • mSystems
  • Log in
  • My alerts
  • My Cart

Main menu

  • Home
  • Articles
    • Current Issue
    • Accepted Manuscripts
    • COVID-19 Special Collection
    • Archive
    • Minireviews
  • For Authors
    • Submit a Manuscript
    • Scope
    • Editorial Policy
    • Submission, Review, & Publication Processes
    • Organization and Format
    • Errata, Author Corrections, Retractions
    • Illustrations and Tables
    • Nomenclature
    • Abbreviations and Conventions
    • Publication Fees
    • Ethics Resources and Policies
  • About the Journal
    • About JCM
    • Editor in Chief
    • Editorial Board
    • For Reviewers
    • For the Media
    • For Librarians
    • For Advertisers
    • Alerts
    • RSS
    • FAQ
  • Subscribe
    • Members
    • Institutions
  • ASM
    • Antimicrobial Agents and Chemotherapy
    • Applied and Environmental Microbiology
    • Clinical Microbiology Reviews
    • Clinical and Vaccine Immunology
    • EcoSal Plus
    • Eukaryotic Cell
    • Infection and Immunity
    • Journal of Bacteriology
    • Journal of Clinical Microbiology
    • Journal of Microbiology & Biology Education
    • Journal of Virology
    • mBio
    • Microbiology and Molecular Biology Reviews
    • Microbiology Resource Announcements
    • Microbiology Spectrum
    • Molecular and Cellular Biology
    • mSphere
    • mSystems

User menu

  • Log in
  • My alerts
  • My Cart

Search

  • Advanced search
Journal of Clinical Microbiology
publisher-logosite-logo

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Accepted Manuscripts
    • COVID-19 Special Collection
    • Archive
    • Minireviews
  • For Authors
    • Submit a Manuscript
    • Scope
    • Editorial Policy
    • Submission, Review, & Publication Processes
    • Organization and Format
    • Errata, Author Corrections, Retractions
    • Illustrations and Tables
    • Nomenclature
    • Abbreviations and Conventions
    • Publication Fees
    • Ethics Resources and Policies
  • About the Journal
    • About JCM
    • Editor in Chief
    • Editorial Board
    • For Reviewers
    • For the Media
    • For Librarians
    • For Advertisers
    • Alerts
    • RSS
    • FAQ
  • Subscribe
    • Members
    • Institutions
Virology

Detection of Anti-Arboviral Immunoglobulin G by Using a Monoclonal Antibody-Based Capture Enzyme-Linked Immunosorbent Assay

Alison J. Johnson, Denise A. Martin, Nick Karabatsos, John T. Roehrig
Alison J. Johnson
Division of Vector-Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, U.S. Department of Health and Human Services, Fort Collins, Colorado 80522
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Denise A. Martin
Division of Vector-Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, U.S. Department of Health and Human Services, Fort Collins, Colorado 80522
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Nick Karabatsos
Division of Vector-Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, U.S. Department of Health and Human Services, Fort Collins, Colorado 80522
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
John T. Roehrig
Division of Vector-Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, U.S. Department of Health and Human Services, Fort Collins, Colorado 80522
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
DOI: 10.1128/JCM.38.5.1827-1831.2000
  • Article
  • Figures & Data
  • Info & Metrics
  • PDF
Loading

Article Figures & Data

Figures

  • Tables
  • Fig. 1.
    • Open in new tab
    • Download powerpoint
    Fig. 1.

    Standardization of serum dilution for the arboviral IgG ELISAs. Antisera to EEE, SLE, and LAC were used to represent their respective viral genera. P/N ratios for each serum dilution were derived by dividing the arithmetic mean OD of the positive serum specimens reacted with viral antigen by the mean OD of the known negative serum specimens reacted with viral antigen.

  • Fig. 2.
    • Open in new tab
    • Download powerpoint
    Fig. 2.

    Comparison of representative IgG ELISA and PRNT results. Scatter plots are of EEE, SLE, and LAC. P/N ratios were determined at a serum dilution of 1:400. A P/N ratio of <2.0 was considered to be a negative result. The PRNT values plotted are of the log10endpoint titers. A PRNT titer of <5 (<log10 0.7) was considered to be a negative result.

Tables

  • Figures
  • Table 1.

    Reagent dilutionsa used in the IgG ELISA

    GenusViral antigen (strain)Dilution
    AntigenPositive serum
    AlphavirusChikungunya (S27)1:5,1201:100
    EEE (NJ/60)1:1,2801:400
    Evergladesb(Fe3-7c)1:101:400
    Mayaro (D218)1:1601:3,200
    Ross River (T48)1:1601:200
    Sindbis (EgAr 339)1:401:100
    VEE (TC-83)1:1201:400
    WEE (Fleming)1:801:100
    FlavivirusDEN2c (New Guinea C)1:2401:200
    Japanese encephalitis (Nakayama)1:3201:400
    MVE (Original)1:6401:400
    Powassan (64-7483)1:401:1,600
    SLE (TBH-28)1:3201:1,600
    WN encephalitis (Eg 101)1:1001:1,200
    Yellow fever (17D)1:2001:400
    BunyavirusCalifornia encephalitis (BFS 283)1:301:400
    LAC (Original)1:201:400
    Snowshoe hare (Original)1:401:400
    Tahyna (Bardos 92)1:201:400
    • ↵a The reagent dilutions pertain only to the specific lot numbers used in this study. New lots require titration to achieve optimum test performance.

    • ↵b Everglades is a VEE virus, subtype 2.

    • ↵c DEN2 viral antigen was used to represent all four DEN serotypes. The IgG ELISA is not specific enough to be able to differentiate between the serotypes.

PreviousNext
Back to top
Download PDF
Citation Tools
Detection of Anti-Arboviral Immunoglobulin G by Using a Monoclonal Antibody-Based Capture Enzyme-Linked Immunosorbent Assay
Alison J. Johnson, Denise A. Martin, Nick Karabatsos, John T. Roehrig
Journal of Clinical Microbiology May 2000, 38 (5) 1827-1831; DOI: 10.1128/JCM.38.5.1827-1831.2000

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Print

Alerts
Sign In to Email Alerts with your Email Address
Email

Thank you for sharing this Journal of Clinical Microbiology article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Detection of Anti-Arboviral Immunoglobulin G by Using a Monoclonal Antibody-Based Capture Enzyme-Linked Immunosorbent Assay
(Your Name) has forwarded a page to you from Journal of Clinical Microbiology
(Your Name) thought you would be interested in this article in Journal of Clinical Microbiology.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Detection of Anti-Arboviral Immunoglobulin G by Using a Monoclonal Antibody-Based Capture Enzyme-Linked Immunosorbent Assay
Alison J. Johnson, Denise A. Martin, Nick Karabatsos, John T. Roehrig
Journal of Clinical Microbiology May 2000, 38 (5) 1827-1831; DOI: 10.1128/JCM.38.5.1827-1831.2000
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Top
  • Article
    • ABSTRACT
    • MATERIALS AND METHODS
    • RESULTS
    • DISCUSSION
    • ACKNOWLEDGMENTS
    • FOOTNOTES
    • REFERENCES
  • Figures & Data
  • Info & Metrics
  • PDF

KEYWORDS

Antibodies, Monoclonal
Antibodies, Viral
Arbovirus Infections
enzyme-linked immunosorbent assay
immunoglobulin G

Related Articles

Cited By...

About

  • About JCM
  • Editor in Chief
  • Board of Editors
  • Editor Conflicts of Interest
  • For Reviewers
  • For the Media
  • For Librarians
  • For Advertisers
  • Alerts
  • RSS
  • FAQ
  • Permissions
  • Journal Announcements

Authors

  • ASM Author Center
  • Submit a Manuscript
  • Article Types
  • Resources for Clinical Microbiologists
  • Ethics
  • Contact Us

Follow #JClinMicro

@ASMicrobiology

       

ASM Journals

ASM journals are the most prominent publications in the field, delivering up-to-date and authoritative coverage of both basic and clinical microbiology.

About ASM | Contact Us | Press Room

 

ASM is a member of

Scientific Society Publisher Alliance

 

American Society for Microbiology
1752 N St. NW
Washington, DC 20036
Phone: (202) 737-3600

 

Copyright © 2021 American Society for Microbiology | Privacy Policy | Website feedback

Print ISSN: 0095-1137; Online ISSN: 1098-660X