Article Figures & Data
Figures
- Fig. 1.
Standardization of serum dilution for the arboviral IgG ELISAs. Antisera to EEE, SLE, and LAC were used to represent their respective viral genera. P/N ratios for each serum dilution were derived by dividing the arithmetic mean OD of the positive serum specimens reacted with viral antigen by the mean OD of the known negative serum specimens reacted with viral antigen.
- Fig. 2.
Comparison of representative IgG ELISA and PRNT results. Scatter plots are of EEE, SLE, and LAC. P/N ratios were determined at a serum dilution of 1:400. A P/N ratio of <2.0 was considered to be a negative result. The PRNT values plotted are of the log10endpoint titers. A PRNT titer of <5 (<log10 0.7) was considered to be a negative result.
Tables
- Table 1.
Reagent dilutionsa used in the IgG ELISA
Genus Viral antigen (strain) Dilution Antigen Positive serum Alphavirus Chikungunya (S27) 1:5,120 1:100 EEE (NJ/60) 1:1,280 1:400 Evergladesb(Fe3-7c) 1:10 1:400 Mayaro (D218) 1:160 1:3,200 Ross River (T48) 1:160 1:200 Sindbis (EgAr 339) 1:40 1:100 VEE (TC-83) 1:120 1:400 WEE (Fleming) 1:80 1:100 Flavivirus DEN2c (New Guinea C) 1:240 1:200 Japanese encephalitis (Nakayama) 1:320 1:400 MVE (Original) 1:640 1:400 Powassan (64-7483) 1:40 1:1,600 SLE (TBH-28) 1:320 1:1,600 WN encephalitis (Eg 101) 1:100 1:1,200 Yellow fever (17D) 1:200 1:400 Bunyavirus California encephalitis (BFS 283) 1:30 1:400 LAC (Original) 1:20 1:400 Snowshoe hare (Original) 1:40 1:400 Tahyna (Bardos 92) 1:20 1:400 ↵a The reagent dilutions pertain only to the specific lot numbers used in this study. New lots require titration to achieve optimum test performance.
↵b Everglades is a VEE virus, subtype 2.
↵c DEN2 viral antigen was used to represent all four DEN serotypes. The IgG ELISA is not specific enough to be able to differentiate between the serotypes.
