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Bacteriology

Rapid Detection of mecA-Positive andmecA-Negative Coagulase-Negative Staphylococci by an Anti-Penicillin Binding Protein 2a Slide Latex Agglutination Test

Zafar Hussain, Luba Stoakes, Shaunalee Garrow, Susan Longo, Viivi Fitzgerald, Robert Lannigan
Zafar Hussain
London Health Sciences Centre and
Department of Microbiology and Immunology, The University of Western Ontario, London, Ontario, Canada
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Luba Stoakes
London Health Sciences Centre and
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Shaunalee Garrow
London Health Sciences Centre and
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Susan Longo
London Health Sciences Centre and
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Viivi Fitzgerald
London Health Sciences Centre and
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Robert Lannigan
London Health Sciences Centre and
Department of Microbiology and Immunology, The University of Western Ontario, London, Ontario, Canada
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DOI: 10.1128/JCM.38.6.2051-2054.2000
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Tables

  • Table 1.

    Comparison of NCCLS breakpoints and LA test results with PCR results

    CategoryNo. of isolates
    TotalOxacillin MICLA test
    ≤0.25 mg/liter≥0.5 mg/literPositiveNegative
    mecA positive25102512510
    mecA negative212129831211
  • Table 2.

    Oxacillin MICs and LA test results after overnight induction with an oxacillin disc

    SpeciesaNo. of isolates
    TotalOxacillin MIC, ≤0.25 mg/literOxacillin MIC, ≥0.5 mg/literPositive LA test result
    S. epidermidis
     mecA+1530153153
     mecA−686800
    S. haemolyticus
     mecA +2502525
     mecA−6600
    S. hominis
     mecA +4704747
     mecA−333300
    S. capitis subsp.capitis
     mecA +0000
     mecA −8800
    S. capitis subsp. urealyticus
     mecA+6066
     mecA −4040
    S. caprae
     mecA+2022
     mecA −6060
    S. cohnii
     mecA+5055
     mecA−15015b0
    S. lugdunensis
     mecA +0000
     mecA −190190
    S. saprophyticus
     mecA +2022
     mecA −190190
    S. schleiferi
     mecA +0000
     mecA −101000
    S. simulans
     mecA +5055
     mecA −532c1
    S. warneri
     mecA+6066
     mecA −8170
    S. xylosus
     mecA+0000
     mecA −110110
    Total463
     mecA+2510251251
     mecA−212129831
    • ↵a mecA +, mecA positive;mecA −, mecA negative.

    • ↵b For one strain the oxacillin MIC was 4 mg/liter.

    • ↵c The oxacillin MICs for one these strains were >4 mg/liter.

  • Table 3.

    MRSA-Screen results for 125 mecA-positive strains with and without oxacillin induction

    SpeciesNo. of isolates
    TotalLA with inductionLA without induction
    Observed at:Not observed
    3 min6 min15 min
    S. epidermidis70701810438
    S. capitissubsp. urealyticus555000
    S. cohnii subsp. cohnii222000
    S. haemolyticus111111000
    S. hominis3333171015
    S. warneri444000
    Total12512541171053
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Rapid Detection of mecA-Positive andmecA-Negative Coagulase-Negative Staphylococci by an Anti-Penicillin Binding Protein 2a Slide Latex Agglutination Test
Zafar Hussain, Luba Stoakes, Shaunalee Garrow, Susan Longo, Viivi Fitzgerald, Robert Lannigan
Journal of Clinical Microbiology Jun 2000, 38 (6) 2051-2054; DOI: 10.1128/JCM.38.6.2051-2054.2000

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Rapid Detection of mecA-Positive andmecA-Negative Coagulase-Negative Staphylococci by an Anti-Penicillin Binding Protein 2a Slide Latex Agglutination Test
Zafar Hussain, Luba Stoakes, Shaunalee Garrow, Susan Longo, Viivi Fitzgerald, Robert Lannigan
Journal of Clinical Microbiology Jun 2000, 38 (6) 2051-2054; DOI: 10.1128/JCM.38.6.2051-2054.2000
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KEYWORDS

Bacterial Proteins
Carrier Proteins
Coagulase
Hexosyltransferases
Latex Fixation Tests
Muramoylpentapeptide Carboxypeptidase
Peptidyl Transferases
Reagent Kits, Diagnostic
Staphylococcus

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