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Bacteriology

Recovery of Bordetella holmesii from Patients with Pertussis-Like Symptoms: Use of Pulsed-Field Gel Electrophoresis To Characterize Circulating Strains

Eyob Mazengia, Ellen A. Silva, Joseph A. Peppe, Ralph Timperi, Harvey George
Eyob Mazengia
Massachusetts State Laboratory Institute, Boston, Massachusetts
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Ellen A. Silva
Massachusetts State Laboratory Institute, Boston, Massachusetts
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Joseph A. Peppe
Massachusetts State Laboratory Institute, Boston, Massachusetts
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Ralph Timperi
Massachusetts State Laboratory Institute, Boston, Massachusetts
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Harvey George
Massachusetts State Laboratory Institute, Boston, Massachusetts
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DOI: 10.1128/JCM.38.6.2330-2333.2000
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    Fig. 1.

    XbaI (A) and SpeI (B) B. holmesii restriction patterns.

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  • Table 1.

    Phenotypic characteristics of B. holmesiiisolates, other representative Bordetella spp., and a nonhemolytic asaccharolytic Acinetobacter

    Testa% of positive isolates (no. of isolates with delayed reactions)
    B. holmesii (n = 32 [nasopharyngeal])bB. pertussis(n = 5)B. parapertussis (n = 5)B. bronchiseptica (n = 4)Asaccharolytic nonhemolyticAcinetobacterc
    Fluorescent-antibody test with:
     B. pertussisconjugate010000NTd
     B. parapertussisconjugate001000NT
    Beta-like hemolysis0100e1005022
    Acid produced from dextrose00000
    Growth on MacConkey agar100f010010073 (4)
    Oxidase010001000.8e
    Urea hydrolysis001001005 (14)
    Motility0001000
    Brown soluble pigment1000100010
    Acinetobactertransformation assay0000100
    • ↵a All isolates tested negative by Gram staining. Except for Gram staining, the oxidase test, theAcinetobacter transformation assay, and the fluorescent-antibody test, test results were read daily. Final readings were taken after 7 days of incubation.

    • ↵b Isolates include two strains previously confirmed at the CDC as B. holmesii. One B. holmesii blood isolate (95R0375) was negative by all tests except the oxidase test (it showed a light reaction) and the brown soluble pigment test.

    • ↵c Data are from reference10.

    • ↵d NT, not tested.

    • ↵e The reaction was weak.

    • ↵f The reaction was light.

  • Table 2.

    Recovery of Bordetella species on selective and nonselective media

    Species (no. tested)% of isolates recovered on:
    CACA-LEXBG agarBG-LEXBG-METBG-OXA
    B. holmesii (33)10001000100100
    B. pertussis(5)100100100NTa100100
    B. parapertussis(5)100100100NT100100
    B. bronchiseptica(4)100100100NT100100
    • ↵a NT, not tested (the effect of cephalexin in BG agar on B. pertussis, B. parapertussis, and B. bronchiseptica was not evaluated since all of them were originally recovered from a medium containing cephalexin).

  • Table 3.

    Bordetella spp. isolated at the SLI from nasopharyngeal cultures

    OrganismNo. of specimens isolated in:Total
    1995199619971998
    B. holmesii3681532
    B. pertussis140325129146740
    B. parapertussis2032113396
     Total2,4623,6772,3432,48410,966
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Recovery of Bordetella holmesii from Patients with Pertussis-Like Symptoms: Use of Pulsed-Field Gel Electrophoresis To Characterize Circulating Strains
Eyob Mazengia, Ellen A. Silva, Joseph A. Peppe, Ralph Timperi, Harvey George
Journal of Clinical Microbiology Jun 2000, 38 (6) 2330-2333; DOI: 10.1128/JCM.38.6.2330-2333.2000

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Recovery of Bordetella holmesii from Patients with Pertussis-Like Symptoms: Use of Pulsed-Field Gel Electrophoresis To Characterize Circulating Strains
Eyob Mazengia, Ellen A. Silva, Joseph A. Peppe, Ralph Timperi, Harvey George
Journal of Clinical Microbiology Jun 2000, 38 (6) 2330-2333; DOI: 10.1128/JCM.38.6.2330-2333.2000
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KEYWORDS

Bordetella
Electrophoresis, Gel, Pulsed-Field
Polymorphism, Restriction Fragment Length
whooping cough

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