DOI: 10.1128/JCM.38.8.2929-2932.2000
Article Figures & Data
Figures
- Fig. 1.
Real-time quantitative LC-PCR test using fluorescence energy transfer. (A) Duplicate amplification plots obtained forT. gondii DNA dilutions from 0.75 × 106(right) to 0.75 parasite in 10 μl (left). Each slope corresponds to a particular input target quantity. (B) Plot of the duplicate Cp against the input target quantity (common log scale) showing the linearity of the results. The computer-calculated correlation coefficient is 1.
Tables
- Table 1.
Main features, pretransplant toxoplasma serological status of the recipient and donor, antitoxoplasma treatment, and outcomes for four allogeneic stem cell transplant recipients with toxoplasma PCR-positive blood samples studied by quantitative LC-PCR testa
Patient no. Sex, age (yr), underlying disease Stem cell transplant type Toxoplasma serological status Graft-versus-host disease Main symptom(s) No. of samples tested (no. of PCR-positive samples) Time (day) of first and last PCR-positive blood samples Antitoxoplasma drug treatment Outcome (cause of death) 613 M, 47, aplastic anemia Familial mismatch R+, D+ Acute II Fever 6 (4) 21–41 TMP-SMZ on days 19–37; Pyr-Clin from day 38 until death Death on day 180 (bacterial sepsis) 685 F, 11, sickle-cell disease HLA-identical sibling R+, D− None Fever 9 (7) 12–38 TMP-SMZ on day 29, continued Alive 696 M, 40, lymphoma HLA-identical sibling R+, D+ Acute I Fever, enlarged lymph nodes 6 (4) 34–45 TMP-SMZ on day 34, continued Alive 708 M, 47, lymphoma Unrelated R+, D− Acute III Fever, behavior trouble, diffuse lesions on cerebral CT scan 9 (8) 61b until death Aerosolized pentamidine on days 50–70; Pyr-Clin on day 75 until death Death on day 109 (coma of uncertain cause)
