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Parasitology

Real-Time PCR for Diagnosis and Follow-Up of Toxoplasma Reactivation after Allogeneic Stem Cell Transplantation Using Fluorescence Resonance Energy Transfer Hybridization Probes

J.-M. Costa, C. Pautas, P. Ernault, F. Foulet, C. Cordonnier, S. Bretagne
J.-M. Costa
Laboratoire de Biologie Moléculaire, Hôpital Américain, BP 109, 92202, Neuilly, and
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C. Pautas
Service d'Hématologie Clinique, Hôpital H. Mondor-APHP, and
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P. Ernault
Laboratoire de Biologie Moléculaire, Hôpital Américain, BP 109, 92202, Neuilly, and
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F. Foulet
Laboratoire de Parasitologie, Hôpital H. Mondor-APHP et UniversitéParis XII, 94010, Créteil, France
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C. Cordonnier
Service d'Hématologie Clinique, Hôpital H. Mondor-APHP, and
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S. Bretagne
Laboratoire de Parasitologie, Hôpital H. Mondor-APHP et UniversitéParis XII, 94010, Créteil, France
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DOI: 10.1128/JCM.38.8.2929-2932.2000
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    Fig. 1.

    Real-time quantitative LC-PCR test using fluorescence energy transfer. (A) Duplicate amplification plots obtained forT. gondii DNA dilutions from 0.75 × 106(right) to 0.75 parasite in 10 μl (left). Each slope corresponds to a particular input target quantity. (B) Plot of the duplicate Cp against the input target quantity (common log scale) showing the linearity of the results. The computer-calculated correlation coefficient is 1.

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    Main features, pretransplant toxoplasma serological status of the recipient and donor, antitoxoplasma treatment, and outcomes for four allogeneic stem cell transplant recipients with toxoplasma PCR-positive blood samples studied by quantitative LC-PCR testa

    Patient no.Sex, age (yr), underlying diseaseStem cell transplant typeToxoplasma serological statusGraft-versus-host diseaseMain symptom(s)No. of samples tested (no. of PCR-positive samples)Time (day) of first and last PCR-positive blood samplesAntitoxoplasma drug treatmentOutcome (cause of death)
    613M, 47, aplastic anemiaFamilial mismatchR+, D+Acute IIFever6 (4)21–41TMP-SMZ on days 19–37; Pyr-Clin from day 38 until deathDeath on day 180 (bacterial sepsis)
    685F, 11, sickle-cell diseaseHLA-identical siblingR+, D−NoneFever9 (7)12–38TMP-SMZ on day 29, continuedAlive
    696M, 40, lymphomaHLA-identical sibling R+, D+Acute IFever, enlarged lymph nodes6 (4)34–45TMP-SMZ on day 34, continuedAlive
    708M, 47, lymphomaUnrelatedR+, D−Acute IIIFever, behavior trouble, diffuse lesions on cerebral CT scan9 (8)61b until deathAerosolized pentamidine on days 50–70; Pyr-Clin on day 75 until deathDeath on day 109 (coma of uncertain cause)
    • ↵a Abbreviations and symbols: AML, acute myeloid leukemia; TMP-SMZ, trimethoprim-sulfamethoxazole; Pyr-Clin, pyrimethamine-clindamycin; R, recipient; D, donor; +, positive; −, negative.

    • ↵b PCR-positive CSF on day 75.

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Real-Time PCR for Diagnosis and Follow-Up of Toxoplasma Reactivation after Allogeneic Stem Cell Transplantation Using Fluorescence Resonance Energy Transfer Hybridization Probes
J.-M. Costa, C. Pautas, P. Ernault, F. Foulet, C. Cordonnier, S. Bretagne
Journal of Clinical Microbiology Aug 2000, 38 (8) 2929-2932; DOI: 10.1128/JCM.38.8.2929-2932.2000

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Real-Time PCR for Diagnosis and Follow-Up of Toxoplasma Reactivation after Allogeneic Stem Cell Transplantation Using Fluorescence Resonance Energy Transfer Hybridization Probes
J.-M. Costa, C. Pautas, P. Ernault, F. Foulet, C. Cordonnier, S. Bretagne
Journal of Clinical Microbiology Aug 2000, 38 (8) 2929-2932; DOI: 10.1128/JCM.38.8.2929-2932.2000
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KEYWORDS

Hematopoietic Stem Cell Transplantation
polymerase chain reaction
Toxoplasma
toxoplasmosis

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