Article Figures & Data
Tables
- Table 1.
Distribution of MICs reported by eight participating facilities each testing two control strains on 10 separate days
Antifungal agent and control straina Incubation time (h) No. of times the following MICs (μg/ml) were reported (n = 240)b: ≤0.016 0.03 0.06 0.12 0.25 0.5 1.0 2.0 4.0 8.0 16 32 64 128 Amphotericin B C. parapsilosis 24 7 [1 120 109 3] 48 20 [1 88 123 8] C. krusei 24 [2 141 97] 48 [10 202 28] Flucytosine C. parapsilosis 24 [43c 152 43] 1 1 48 4c [12 189 34] 1 C. krusei 24 1 5 [36 119 79] 48 1 [74 162 3] Fluconazoled C. parapsilosis 24 1 2 [4 55 91 15] 48 1 2 1 [42 119 41] C. krusei 24 [5 95 76 4] 48 [4 108 98 0] Voriconazole C. parapsilosis 24 [9 86 141 4] 48 [21 113 83 3] C. krusei 24 4 [17 76 139 4] 48 [0 110 125 5] Ketoconazole C. parapsilosis 24 6 [7 106 108 13] 48 2 [23 114 82 17] 2 C. krusei 24 1 10 [32 74 123 0] 48 [25 143 70] 1 Itraconazole C. parapsilosis 24 5 4 [34 129 66] 1 48 5 [28 149 57] 1 C. krusei 24 1 9 [23 68 136 3] 48 [23 170 46] Caspofungin (MK0991) C. parapsilosis 24 6 1 1 [29 159 44] 48 1 5 [11 143 67 2] 9 2c C. krusei 24 3 [22 98 116 1] 48 4 [22 164 48] 2 Ravuconazole (BMS 207147) C. parapsilosis 24 [9 93 121 7] 10 48 3 [8 114 92 22] 1 C. krusei 24 6 10 [17 72 96 39] 48 [83 152 5] Posaconazole (SCH 56592) C. parapsilosis 24 7 [36 119 77] 1 48 1 1 [15 161 61] 1 C. krusei 24 [12 51 107 70] 48 1 [3 93 140 3] LY 303366 C. parapsilosis 24 4 3 2 [43 111 77 0] 48 1 [31 83 123 2] C. krusei 24 1 [8 130 75 23] 2 1 48 1 2 [59 108 44 14] 10 2 ↵a C. parapsilosis ATCC 22019 and C. krusei ATCC 6258.
↵b Proposed QC limits are indicated by brackets.
↵c MICs outside the range of flucytosine or caspofungin that were actually tested.
↵d Data from one laboratory were excluded because MICs of the control drug, amphotericin B, were outside of the proposed limits; another laboratory reported only 48-h readings. A third laboratory experienced growth failures with two lots of broth, especially after 24 h. Consequently, the total number of evaluable fluconazole MICs was less than the 240 expected test results.
- Table 2.
Proposed MIC ranges of various antifungal agents for two quality control strains of Candida spp. when tested by the NCCLS microdilution procedure
Antifungal agent MIC (μg/ml) ranges for microdilution tests with: C. parapsilosisATCC 22019 at: C. krusei ATCC 6258 at: 24 h 48 h 24 h 48 h Amphotericin B 0.25–2.0 0.5–4.0 0.5–2.0 1.0–4.0 Flucytocine 0.06–0.25 0.12–0.5 4.0–16 8.0–32 Fluconazole 0.5–4.0 1.0–4.0 8.0–64 16–128 Voriconazole 0.016–0.12 0.03–0.25 0.06–0.5 0.12–1.0 Ketoconazole 0.03–0.25 0.06–0.5 0.12–1.0 0.25–1.0 Itraconazole 0.12–0.5 0.12–0.5 0.12–1.0 0.25–1.0 Caspofungin (MK0991) 0.25–1.0 0.5–4.0 0.12–1.0 0.25–1.0 Ravuconazole (BMS 207147) 0.016–0.12 0.03–0.25 0.06–0.5 0.25–1.0 Posaconazole (SCH 56592) 0.06–0.25 0.06–0.25 0.06–0.5 0.12–1.0 LY303366 1.0–8.0 1.0–8.0 0.03–0.25 0.06–0.5