Skip to main content
  • ASM
    • Antimicrobial Agents and Chemotherapy
    • Applied and Environmental Microbiology
    • Clinical Microbiology Reviews
    • Clinical and Vaccine Immunology
    • EcoSal Plus
    • Eukaryotic Cell
    • Infection and Immunity
    • Journal of Bacteriology
    • Journal of Clinical Microbiology
    • Journal of Microbiology & Biology Education
    • Journal of Virology
    • mBio
    • Microbiology and Molecular Biology Reviews
    • Microbiology Resource Announcements
    • Microbiology Spectrum
    • Molecular and Cellular Biology
    • mSphere
    • mSystems
  • Log in
  • My alerts
  • My Cart

Main menu

  • Home
  • Articles
    • Current Issue
    • Accepted Manuscripts
    • Archive
    • Minireviews
  • For Authors
    • Submit a Manuscript
    • Scope
    • Editorial Policy
    • Submission, Review, & Publication Processes
    • Organization and Format
    • Errata, Author Corrections, Retractions
    • Illustrations and Tables
    • Nomenclature
    • Abbreviations and Conventions
    • Publication Fees
    • Ethics Resources and Policies
  • About the Journal
    • About JCM
    • Editor in Chief
    • Editorial Board
    • For Reviewers
    • For the Media
    • For Librarians
    • For Advertisers
    • Alerts
    • RSS
    • FAQ
  • Subscribe
    • Members
    • Institutions
  • ASM
    • Antimicrobial Agents and Chemotherapy
    • Applied and Environmental Microbiology
    • Clinical Microbiology Reviews
    • Clinical and Vaccine Immunology
    • EcoSal Plus
    • Eukaryotic Cell
    • Infection and Immunity
    • Journal of Bacteriology
    • Journal of Clinical Microbiology
    • Journal of Microbiology & Biology Education
    • Journal of Virology
    • mBio
    • Microbiology and Molecular Biology Reviews
    • Microbiology Resource Announcements
    • Microbiology Spectrum
    • Molecular and Cellular Biology
    • mSphere
    • mSystems

User menu

  • Log in
  • My alerts
  • My Cart

Search

  • Advanced search
Journal of Clinical Microbiology
publisher-logosite-logo

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Accepted Manuscripts
    • Archive
    • Minireviews
  • For Authors
    • Submit a Manuscript
    • Scope
    • Editorial Policy
    • Submission, Review, & Publication Processes
    • Organization and Format
    • Errata, Author Corrections, Retractions
    • Illustrations and Tables
    • Nomenclature
    • Abbreviations and Conventions
    • Publication Fees
    • Ethics Resources and Policies
  • About the Journal
    • About JCM
    • Editor in Chief
    • Editorial Board
    • For Reviewers
    • For the Media
    • For Librarians
    • For Advertisers
    • Alerts
    • RSS
    • FAQ
  • Subscribe
    • Members
    • Institutions
Epidemiology

Cattle Can Be a Reservoir of Sorbitol-Fermenting Shiga Toxin-Producing Escherichia coli O157:H−Strains and a Source of Human Diseases

Martina Bielaszewska, Herbert Schmidt, Almut Liesegang, Rita Prager, Wolfgang Rabsch, Helmut Tschäpe, Alois Cízek, Jan Janda, Kveta Bláhová, Helge Karch
Martina Bielaszewska
Institute for Medical Microbiology, The 2nd Medical Faculty, Charles University, 1 and Institut für Hygiene und Mikrobiologie der Universität Würzburg, 97080 Würzburg, 2 and
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Herbert Schmidt
Institut für Hygiene und Mikrobiologie der Universität Würzburg, 97080 Würzburg, 2 and
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Almut Liesegang
Robert Koch Institut, Bereich Wernigerode, 38855 Wernigerode, 3 Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Rita Prager
Robert Koch Institut, Bereich Wernigerode, 38855 Wernigerode, 3 Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Wolfgang Rabsch
Robert Koch Institut, Bereich Wernigerode, 38855 Wernigerode, 3 Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Helmut Tschäpe
Robert Koch Institut, Bereich Wernigerode, 38855 Wernigerode, 3 Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Alois Cízek
Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences, 602 00 Brno, 4 Czech Republic, and
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jan Janda
1st Clinic of Pediatrics, University Hospital Motol, 5 150 06 Prague, and
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Kveta Bláhová
1st Clinic of Pediatrics, University Hospital Motol, 5 150 06 Prague, and
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Helge Karch
Institut für Hygiene und Mikrobiologie der Universität Würzburg, 97080 Würzburg, 2 and
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
DOI: 
  • Article
  • Figures & Data
  • Info & Metrics
  • PDF
Loading

Article Figures & Data

Figures

  • Tables
  • Fig. 1.
    • Open in new tab
    • Download powerpoint
    Fig. 1.

    Agarose gel electrophoresis of fliC PCR products of SF STEC O157:H− strains and of controlE. coli O157 strains after restriction with RsaI. Lanes M, molecular size marker (1-kb DNA ladder; Gibco BRL). In lanes 1 to 7, the following SF STEC O157:H− strains are shown: lane 1, 550/98; lane 2, 258/98; lane 3, 269/98; lane 4, 703/88; lane 5, 221/95; lane 6, 1995/96; and lane 7, 3573/98. Lanes 8 to 10 contain control E. coli O157 strains as follows: lane 8, EDL 933 (O157:H7); lane 9, 1083-36/91 (O157:H45); and lane 10, 693/91 (O157:H19).

  • Fig. 2.
    • Open in new tab
    • Download powerpoint
    Fig. 2.

    PFGE patterns of XbaI-digested genomic DNAs of SF STEC O157:H− strains and control E. coli O157 strains. Lane S, molecular size standard (DNA from E. coli strain G5244 restricted withXbaI). In lanes 1 to 7, the following SF STEC O157:H− strains are shown: lane 1, 258/98; lane 2, 269/98; lane 3, 550/98; lane 4, 221/95; lane 5, 703/88; lane 6, 1995/96; and lane 7, 3573/98. In lanes 8 to 10, control E. coli O157 strains are shown as follows: lane 8, EDL933 (NSF STEC O157:H7); lane 9, 1083-36/91 (SF, Stx-negative E. coli O157:H45); and lane 10, 693/91 (SF, Stx-negative E. coli O157:H19).

  • Fig. 3.
    • Open in new tab
    • Download powerpoint
    Fig. 3.

    Cluster analysis, derived from PFGE data, of SF STEC O157:H− isolates from the cow and patients, NSF STEC O157:H7 strain EDL 933, and SF Stx-negative strains O157:H45 and O157:H19 with the RFLPscan software.

Tables

  • Figures
  • Table 1.

    Characteristics of SF STEC O157:H− strains isolated from a cow and patients and of control NSF STEC O157:H7 strain EDL 933

    StrainaDisease; countryb (referencec)Phenotypic characteristicsdChromosomal characteristicsPlasmid-encoded genesg
    SerotypePTSF/GUDStxEHEC Hlystxe eaeP-gene profilefEHEChlykatPespPetp
    258/98h HUS; CRO157:H− 88+/+2− stx 2/18.0γ17−/−−/−−/−−/−
    269/98h WD; CRO157:H− 88+/+2− stx 2/18.0γ17−/−−/−−/−−/−
    550/98h Cow; CRO157:H− 88+/+2− stx 2/18.0γ17−/−−/−−/−−/−
    703/88HUS; G (10)O157:H− 88+/+2− stx 2/18.0γ17+/48.5−/−−/−+/3.9, 1.9i
    221/95HUS; CR (2)O157:H− 88+/+2− stx 2/18.0γ9+/15.0−/−−/−+/3.7, 1.7
    1995/96HUS; G (2)O157:H− 88+/+2− stx 2/18.0γ16+/15.0−/−−/−+/3.9, 1.9
    3573/98HUS; GO157:H− 88+/+2− stx 2/18.0γ16+/15.0−/−−/−+/3.9, 1.9
    EDL 933HC; US (15)O157:H721−/−1 and 2+ stx 1/NPγ2+/12.0+/9.0+/7.5+/3.9, 1.9
    stx 2/4.7
    • ↵a For the SF STEC O157:H−strains, the last two numbers indicate the year of isolation.

    • ↵b WD, watery diarrhea; HC, hemorrhagic colitis; CR, Czech Republic; G, Germany; US, United States.

    • ↵c Strains for which no references are given are from this study.

    • ↵d PT, phage type; SF/GUD, sorbitol fermentation/β-d-glucuronidase activity; Stx, Stx phenotype; EHEC Hly, production of EHEC hemolysin; +, positive result; −, negative result.

    • ↵e stx PCR result/size (in kilobases) of EcoRI restriction fragment of the genomic DNA hybridizing to the stx 2 probe. NP, not performed.

    • ↵f P-gene profile 17 is characterized by three fragments of 20, 6.4, and 3.7 kb; the relatedP-gene profile 16 is characterized by two fragments of 20 and 3.7 kb, and the other related P-gene profile 9 is characterized by two fragments of 6.4 and 3.7 kb. P-gene profile 2 consists of two fragments of 21.2 and 16.2 kb and is unrelated to P-gene profiles 17, 16, and 9.

    • ↵g Detection of the gene by PCR/size (in kilobases) of the plasmid DNA fragment hybridizing to the respective probe. +, positive result; −, no signal obtained.

    • ↵h Epidemiologically related strains; the other four SF STEC O157:H− strains are representative isolates from 1988 to 1998 and are epidemiologically unrelated.

    • ↵i Fragments of two different sizes hybridized with the probe.

PreviousNext
Back to top
Download PDF
Citation Tools
Cattle Can Be a Reservoir of Sorbitol-Fermenting Shiga Toxin-Producing Escherichia coli O157:H−Strains and a Source of Human Diseases
Martina Bielaszewska, Herbert Schmidt, Almut Liesegang, Rita Prager, Wolfgang Rabsch, Helmut Tschäpe, Alois Cízek, Jan Janda, Kveta Bláhová, Helge Karch
Journal of Clinical Microbiology Sep 2000, 38 (9) 3470-3473; DOI:

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Print

Alerts
Sign In to Email Alerts with your Email Address
Email

Thank you for sharing this Journal of Clinical Microbiology article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Cattle Can Be a Reservoir of Sorbitol-Fermenting Shiga Toxin-Producing Escherichia coli O157:H−Strains and a Source of Human Diseases
(Your Name) has forwarded a page to you from Journal of Clinical Microbiology
(Your Name) thought you would be interested in this article in Journal of Clinical Microbiology.
Share
Cattle Can Be a Reservoir of Sorbitol-Fermenting Shiga Toxin-Producing Escherichia coli O157:H−Strains and a Source of Human Diseases
Martina Bielaszewska, Herbert Schmidt, Almut Liesegang, Rita Prager, Wolfgang Rabsch, Helmut Tschäpe, Alois Cízek, Jan Janda, Kveta Bláhová, Helge Karch
Journal of Clinical Microbiology Sep 2000, 38 (9) 3470-3473; DOI:
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Top
  • Article
    • ABSTRACT
    • ACKNOWLEDGMENTS
    • FOOTNOTES
    • REFERENCES
  • Figures & Data
  • Info & Metrics
  • PDF

Related Articles

Cited By...

About

  • About JCM
  • Editor in Chief
  • Board of Editors
  • Editor Conflicts of Interest
  • For Reviewers
  • For the Media
  • For Librarians
  • For Advertisers
  • Alerts
  • RSS
  • FAQ
  • Permissions
  • Journal Announcements

Authors

  • ASM Author Center
  • Submit a Manuscript
  • Article Types
  • Resources for Clinical Microbiologists
  • Ethics
  • Contact Us

Follow #JClinMicro

@ASMicrobiology

       

ASM Journals

ASM journals are the most prominent publications in the field, delivering up-to-date and authoritative coverage of both basic and clinical microbiology.

About ASM | Contact Us | Press Room

 

ASM is a member of

Scientific Society Publisher Alliance

Copyright © 2019 American Society for Microbiology | Privacy Policy | Website feedback

Print ISSN: 0095-1137; Online ISSN: 1098-660X