Article Figures & Data
Figures
- Fig. 1.
Agarose gel electrophoresis of fliC PCR products of SF STEC O157:H− strains and of controlE. coli O157 strains after restriction with RsaI. Lanes M, molecular size marker (1-kb DNA ladder; Gibco BRL). In lanes 1 to 7, the following SF STEC O157:H− strains are shown: lane 1, 550/98; lane 2, 258/98; lane 3, 269/98; lane 4, 703/88; lane 5, 221/95; lane 6, 1995/96; and lane 7, 3573/98. Lanes 8 to 10 contain control E. coli O157 strains as follows: lane 8, EDL 933 (O157:H7); lane 9, 1083-36/91 (O157:H45); and lane 10, 693/91 (O157:H19).
- Fig. 2.
PFGE patterns of XbaI-digested genomic DNAs of SF STEC O157:H− strains and control E. coli O157 strains. Lane S, molecular size standard (DNA from E. coli strain G5244 restricted withXbaI). In lanes 1 to 7, the following SF STEC O157:H− strains are shown: lane 1, 258/98; lane 2, 269/98; lane 3, 550/98; lane 4, 221/95; lane 5, 703/88; lane 6, 1995/96; and lane 7, 3573/98. In lanes 8 to 10, control E. coli O157 strains are shown as follows: lane 8, EDL933 (NSF STEC O157:H7); lane 9, 1083-36/91 (SF, Stx-negative E. coli O157:H45); and lane 10, 693/91 (SF, Stx-negative E. coli O157:H19).
- Fig. 3.
Cluster analysis, derived from PFGE data, of SF STEC O157:H− isolates from the cow and patients, NSF STEC O157:H7 strain EDL 933, and SF Stx-negative strains O157:H45 and O157:H19 with the RFLPscan software.
Tables
- Table 1.
Characteristics of SF STEC O157:H− strains isolated from a cow and patients and of control NSF STEC O157:H7 strain EDL 933
Straina Disease; countryb (referencec) Phenotypic characteristicsd Chromosomal characteristics Plasmid-encoded genesg Serotype PT SF/GUD Stx EHEC Hly stxe eae P-gene profilef EHEChly katP espP etp 258/98h HUS; CR O157:H− 88 +/+ 2 − stx 2/18.0 γ 17 −/− −/− −/− −/− 269/98h WD; CR O157:H− 88 +/+ 2 − stx 2/18.0 γ 17 −/− −/− −/− −/− 550/98h Cow; CR O157:H− 88 +/+ 2 − stx 2/18.0 γ 17 −/− −/− −/− −/− 703/88 HUS; G (10) O157:H− 88 +/+ 2 − stx 2/18.0 γ 17 +/48.5 −/− −/− +/3.9, 1.9i 221/95 HUS; CR (2) O157:H− 88 +/+ 2 − stx 2/18.0 γ 9 +/15.0 −/− −/− +/3.7, 1.7 1995/96 HUS; G (2) O157:H− 88 +/+ 2 − stx 2/18.0 γ 16 +/15.0 −/− −/− +/3.9, 1.9 3573/98 HUS; G O157:H− 88 +/+ 2 − stx 2/18.0 γ 16 +/15.0 −/− −/− +/3.9, 1.9 EDL 933 HC; US (15) O157:H7 21 −/− 1 and 2 + stx 1/NP γ 2 +/12.0 +/9.0 +/7.5 +/3.9, 1.9 stx 2/4.7 ↵a For the SF STEC O157:H−strains, the last two numbers indicate the year of isolation.
↵b WD, watery diarrhea; HC, hemorrhagic colitis; CR, Czech Republic; G, Germany; US, United States.
↵c Strains for which no references are given are from this study.
↵d PT, phage type; SF/GUD, sorbitol fermentation/β-d-glucuronidase activity; Stx, Stx phenotype; EHEC Hly, production of EHEC hemolysin; +, positive result; −, negative result.
↵e stx PCR result/size (in kilobases) of EcoRI restriction fragment of the genomic DNA hybridizing to the stx 2 probe. NP, not performed.
↵f P-gene profile 17 is characterized by three fragments of 20, 6.4, and 3.7 kb; the relatedP-gene profile 16 is characterized by two fragments of 20 and 3.7 kb, and the other related P-gene profile 9 is characterized by two fragments of 6.4 and 3.7 kb. P-gene profile 2 consists of two fragments of 21.2 and 16.2 kb and is unrelated to P-gene profiles 17, 16, and 9.
↵g Detection of the gene by PCR/size (in kilobases) of the plasmid DNA fragment hybridizing to the respective probe. +, positive result; −, no signal obtained.
↵h Epidemiologically related strains; the other four SF STEC O157:H− strains are representative isolates from 1988 to 1998 and are epidemiologically unrelated.
↵i Fragments of two different sizes hybridized with the probe.
