Parasitology

TaqMan-Based Detection of Trichomonas vaginalis DNA from Female Genital Specimens

Jeanne A. Jordan, Donna Lowery, Massimo Trucco
DOI: 10.1128/JCM.39.11.3819-3822.2001

Article Figures & Data

Figures

  • Fig. 1.
    Fig. 1.

    The calculated ΔRQ values from 552 female genital specimens are plotted based upon established cutoff values. There were 494 PCR-negative (circles) results and 58 PCR-positive (squares) results.

Tables

  • Table 1.

    Comparison of TaqMan-coupled PCR and broth culture for detection of T. vaginalis from female genital specimensa

    PCR resultNo. with broth culture resultTotal
    PositiveNegative
    Positive44 14b 58
    Negative1493494
     Total45507552

    • ↵a Sensitivity, 97.8%; specificity, 97.4%; positive predictive value, 77.6%; negative predictive value, 99.8%; prevalence by culture, 8.2%; prevalence by PCR, 10.5%.

    • ↵b One PCR-positive, culture-negative specimen contained motile, characteristic trichomonads by direct microscopic examination.

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KEYWORDS

DNA, Protozoan
polymerase chain reaction
Taq Polymerase
Trichomonas Vaginitis
Trichomonas vaginalis
Vagina

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