DOI: 10.1128/JCM.39.11.4076-4081.2001
Article Figures & Data
Figures
- Fig. 1.
Allele size distribution at the microsatellite lociCDC3 (A), EF3 (B), andHIS3 (C) upon analysis of 200 alleles of 73 C. albicans isolates and 27 reference strains.
Tables
- Table 1.
Features of the three sets of primers retained in the upstream sequence of the elongation factor 3 gene (EF3), downstream of the cell division cycle protein gene (CDC3), and downstream of the coding sequence for the imidazole glycerol phosphate deshydratase gene (HIS3)
Locus (GenBank accession no.), chromosome Microsatellite sequence Primer sequences (forward and reverse) CDC3 (Z25869 ), chromosome 1 (AGTA)8 5′-CAGATGATTTTTTGTATGAGAAGAA-3′ 5′-CAGTCACAAGATTAAAATGTTCAAG-3′ EF3(Z11484 ), chromosome 5 (TTTC)5(TTC)5 5′-TTTCCTCTTCCTTTCATATAGAA-3′ 5′-GGATTCACTAGCAGCAGACA-3′ HIS3(AF006605 ), chromosome 2 (ATTT)13 5′-TGGCAAAAATGATATTCCAA-3′ 5′-TACACTATGCCCCAAACACA-3′ - Table 2.
Origin and genotype of the 100 isolates tested, including 27 reference strains and 73 independent clinical isolates with the number of isolates (n) with the same profile for calculation of the discriminatory power
Isolate origin Length (bp) determined by PCR analysis of: n CDC3 marker EF3 marker HIS3 marker Allele 1a Allele 2 Allele 1 Allele 2 Allele 1 Allele 2 Reference strain Ca4918 113 113 130 136 150 162 1 Anal swab 113 117 130 130 150 166 1 Abdominal drainage 113 117 130 136 150 162 1 Abdominal drainage 113 117 130 136 150 162 3 Expectoration 113 117 130 136 150 162 Reference strain IP887/65 113 117 130 136 150 162 Stools 113 117 130 136 150 166 1 Oral mucosa 113 117 130 136 162 162 1 Vascular catheter 117 117 126 126 162 162 1 Expectoration 117 117 130 136 150 162 1 Urine 117 117 130 136 154 194 1 Reference strain IP1548/84 117 117 130 136 178 178 2 Reference strain IP1407/82 117 117 130 136 178 178 Anal swab 117 117 131 131 162 162 1 Urine 117 117 131 131 162 182 1 Tracheal aspirate 117 117 133 140 154 154 1 Reference strain 38696 117 117 133 144 154 154 1 Tracheal aspirate 117 117 133 144 154 186 2 Stools 117 117 133 144 154 186 Blood culture 117 117 136 136 150 154 1 Anal swab 117 117 137 139 182 182 1 Stools 117 117 146 146 154 154 1 Abdominal drainage 117 121 133 136 154 154 1 Reference strain IP1876/89 117 121 135 135 162 162 2 Reference strain IP1877/89 117 121 135 135 162 162 Anal swab 117 121 136 136 150 150 1 Urine 117 125 126 126 162 162 1 Stools 117 125 126 126 162 238 1 Anal swab 117 125 126 131 162 170 1 Anal swab 117 125 126 135 150 150 1 Tracheal aspirate 117 125 126 135 158 158 1 Vascular catheter 117 125 126 135 162 162 17 Blood culture 117 125 126 135 162 162 Anal swab 117 125 126 135 162 162 Abdominal drainage 117 125 126 135 162 162 Tracheal aspirate 117 125 126 135 162 162 Stools 117 125 126 135 162 162 Stools 117 125 126 135 162 162 Surgical wound 117 125 126 135 162 162 Tracheal aspirate 117 125 126 135 162 162 Reference strain SC5314 117 125 126 135 162 162 Reference strain 3153A 117 125 126 135 162 162 Reference strain 441B 117 125 126 135 162 162 Reference strain A81Pu 117 125 126 135 162 162 Reference strain IP884/65 117 125 126 135 162 162 Reference strain IP993/69 117 125 126 135 162 162 Reference strain IP996/69 117 125 126 135 162 162 Reference strain IP2146/93 117 125 126 135 162 162 Vascular catheter 117 125 126 135 162 170 1 Stools 117 125 126 135 162 182 1 Stools 117 125 126 135 162 194 2 Oral mucosa 117 125 126 135 162 194 Stools 117 125 126 135 162 198 1 Stools 117 125 126 135 162 206 1 Vascular catheter 117 125 126 135 162 210 1 Vascular catheter 117 125 126 135 162 214 1 Stools 117 125 126 135 162 218 1 Stools 117 125 126 135 170 218 1 Vascular catheter 117 125 126 135 210 210 2 Reference strain 1332/82 117 125 126 135 210 210 Tracheal aspirate 117 125 130 144 154 154 1 Stools 117 125 131 131 162 162 4 Anal swab 117 125 131 131 162 162 Reference strain IP1880/89 117 125 131 131 162 162 Reference strain IP1878/89 117 125 131 131 162 162 Vascular catheter 117 125 131 131 162 186 1 Surgical wound 117 125 131 131 162 190 2 Stools 117 125 131 131 162 190 Vascular catheter 117 125 131 131 166 166 2 Skin 117 125 131 131 166 166 Vascular catheter 117 125 131 131 166 186 2 Blood culture 117 125 131 131 166 186 Bile aspirate 117 125 133 144 154 182 1 Anal swab 117 125 136 139 154 154 2 Blood culture 117 125 136 139 154 154 Stools 117 129 130 145 170 170 1 Urine 117 129 136 146 154 154 2 Blood culture 117 129 136 146 154 154 Urine 117 129 136 146 154 170 1 Reference strain IP1213/80 121 121 130 130 154 154 1 Vaginal swab 121 121 130 131 154 154 1 Anal swab 121 121 130 144 154 166 2 Stools 121 121 130 144 154 166 Stools 121 121 130 145 154 166 1 Reference strain IP1180/79 121 121 131 131 178 178 1 Reference strain IP1663/86 121 121 131 135 170 170 1 Reference strain H12 121 125 130 144 154 154 1 Reference strain B311 121 125 130 144 154 166 1 Tracheal aspirate 125 125 130 130 154 166 1 Reference strain B792 125 125 131 139 166 178 3 Reference strain IP886/65 125 125 131 139 166 178 Reference strain 28367 125 125 131 139 166 178 Blood culture 125 125 131 139 186 186 1 Stools 125 125 131 139 194 194 1 Abdominal drainage 125 125 136 136 150 150 1 Reference strain 10231 125 125 139 139 190 190 1 Oral mucosa 125 129 131 139 186 194 1 Stools 129 129 136 141 150 162 2 Abdominal drainage 129 129 136 141 150 162 Vascular catheter 129 129 136 141 150 166 1 ↵a The terms allele 1 and allele 2 indicate two different alleles for a given locus.
- Table 3.
Comparison of 17 pairs of isolates from blood or central catheter and the corresponding isolates from peripheral anatomical sites collected at the same timea
Patient Isolate origin Length (bp) determined by PCR analysis CDC3 marker EF3 marker HIS3 marker Allele 1b Allele 2 Allele 1 Allele 2 Allele 1 Allele 2 Patient 1 Blood culture 125 125 136 136 150 150 Surgical wound 125 125 136 136 150 150 Patient 2 Central catheter 117 117 126 126 162 162 Oral mucosa 117 117 126 126 162 162 Patient 3 Blood culture 117 117 131 131 162 182 Urine 117 117 131 131 162 182 Patient 4 Blood culture 125 129 131 139 186 194 Oral mucosa 125 129 131 139 186 194 Patient 5 Blood culture 117 125 131 131 166 186 Stools 117 125 131 131 166 186 Patient 6 Central catheter 117 125 126 135 158 158 Tracheal aspirate 117 125 126 135 158 158 Patient 7 Central catheter 117 129 136 146 154 170 Urine 117 125 131 131 190 190 Patient 8 Central catheter 113 117 130 136 162 162 Oral mucosa 113 117 130 136 162 162 Patient 9 Blood culture 117 125 126 135 162 210 Urine 117 125 126 135 162 210 Patient 10 Central catheter 117 125 126 135 210 210 Rectal swab 117 125 126 135 210 210 Patient 11 Central catheter 129 129 136 141 150 166 Tracheal aspirate 129 129 136 141 150 166 Patient 12 Central catheter 117 125 126 135 162 194 Oral mucosa 117 125 126 135 162 194 Patient 13 Blood culture 125 125 131 139 186 186 Stools 125 125 131 139 186 186 Patient 14 Blood culture 117 125 126 135 162 214 Urine 117 125 126 135 162 214 Patient 15 Blood culture 121 121 130 144 154 166 Stools 121 121 130 144 154 166 Patient 16 Central catheter 117 125 131 131 162 186 Tracheal aspirate 117 125 131 131 162 186 Patient 17 Central catheter 117 125 126 135 162 162 Skin 117 129 136 146 154 154
