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Bacteriology

PCR-Based Detection and Identification ofBurkholderiacepacia Complex Pathogens in Sputum from Cystic Fibrosis Patients

Andrew McDowell, Eshwar Mahenthiralingam, John E. Moore, Kerstin E. A. Dunbar, A. Kevin Webb, Mary E. Dodd, S. Lorraine Martin, B. Cherie Millar, Christopher J. Scott, Mary Crowe, J. Stuart Elborn
Andrew McDowell
Molecular Epidemiology Research Unit, Northern Ireland Public Health Laboratory, Department of Bacteriology, and
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Eshwar Mahenthiralingam
Cardiff School of Biosciences, Cardiff University, Cardiff, Wales, United Kingdom CF1 3US;
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John E. Moore
Molecular Epidemiology Research Unit, Northern Ireland Public Health Laboratory, Department of Bacteriology, and
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Kerstin E. A. Dunbar
Molecular Epidemiology Research Unit, Northern Ireland Public Health Laboratory, Department of Bacteriology, and
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A. Kevin Webb
Bradbury Adult Cystic Fibrosis Center, Wythenshawe Hospital, Manchester, England, United Kingdom M23 9LT; and
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Mary E. Dodd
Bradbury Adult Cystic Fibrosis Center, Wythenshawe Hospital, Manchester, England, United Kingdom M23 9LT; and
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S. Lorraine Martin
Biomolecular Sciences Group, School of Pharmacy, The Queen's University of Belfast, Belfast, Northern Ireland, United Kingdom BT9 7BL
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B. Cherie Millar
Molecular Epidemiology Research Unit, Northern Ireland Public Health Laboratory, Department of Bacteriology, and
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Christopher J. Scott
Biomolecular Sciences Group, School of Pharmacy, The Queen's University of Belfast, Belfast, Northern Ireland, United Kingdom BT9 7BL
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Mary Crowe
Molecular Epidemiology Research Unit, Northern Ireland Public Health Laboratory, Department of Bacteriology, and
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J. Stuart Elborn
Northern Ireland Regional Adult Cystic Fibrosis Center, Belfast City Hospital, Belfast, Northern Ireland, United Kingdom BT9 7AB;
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DOI: 10.1128/JCM.39.12.4247-4255.2001
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Article Information

DOI 
https://doi.org/10.1128/JCM.39.12.4247-4255.2001
PubMed 
11724828

Published By 
American Society for Microbiology Journals
History 
  • Received June 22, 2001
  • Returned for modification August 5, 2001
  • Accepted September 8, 2001
  • Published online December 1, 2001.

Copyright & Usage 
Copyright © 2001 American Society for Microbiology

Author Information

  1. Andrew McDowell1*,
  2. Eshwar Mahenthiralingam2,
  3. John E. Moore1,
  4. Kerstin E. A. Dunbar1,
  5. A. Kevin Webb3,
  6. Mary E. Dodd3,
  7. S. Lorraine Martin4,
  8. B. Cherie Millar1,
  9. Christopher J. Scott4,
  10. Mary Crowe1 and
  11. J. Stuart Elborn5
  1. Molecular Epidemiology Research Unit, Northern Ireland Public Health Laboratory, Department of Bacteriology,1 and
  2. Northern Ireland Regional Adult Cystic Fibrosis Center,5 Belfast City Hospital, Belfast, Northern Ireland, United Kingdom BT9 7AB;
  3. Cardiff School of Biosciences, Cardiff University, Cardiff, Wales, United Kingdom CF1 3US2;
  4. Bradbury Adult Cystic Fibrosis Center, Wythenshawe Hospital, Manchester, England, United Kingdom M23 9LT3; and
  5. Biomolecular Sciences Group, School of Pharmacy, The Queen's University of Belfast, Belfast, Northern Ireland, United Kingdom BT9 7BL4
  • ↵* Corresponding author. Present address: Biomolecular Sciences Group, School of Pharmacy, The Queen's University of Belfast, Medical Biology Center, 97 Lisburn Rd., Belfast, Northern Ireland, United Kingdom BT9 7BL. Phone: 44 (0)2890 272047. Fax: 44 (0)2890 247794. E-mail: a.mcdowell{at}qub.ac.uk.

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PCR-Based Detection and Identification ofBurkholderiacepacia Complex Pathogens in Sputum from Cystic Fibrosis Patients
Andrew McDowell, Eshwar Mahenthiralingam, John E. Moore, Kerstin E. A. Dunbar, A. Kevin Webb, Mary E. Dodd, S. Lorraine Martin, B. Cherie Millar, Christopher J. Scott, Mary Crowe, J. Stuart Elborn
Journal of Clinical Microbiology Dec 2001, 39 (12) 4247-4255; DOI: 10.1128/JCM.39.12.4247-4255.2001

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PCR-Based Detection and Identification ofBurkholderiacepacia Complex Pathogens in Sputum from Cystic Fibrosis Patients
Andrew McDowell, Eshwar Mahenthiralingam, John E. Moore, Kerstin E. A. Dunbar, A. Kevin Webb, Mary E. Dodd, S. Lorraine Martin, B. Cherie Millar, Christopher J. Scott, Mary Crowe, J. Stuart Elborn
Journal of Clinical Microbiology Dec 2001, 39 (12) 4247-4255; DOI: 10.1128/JCM.39.12.4247-4255.2001
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KEYWORDS

Burkholderia cepacia
cystic fibrosis
polymerase chain reaction
Polymorphism, Restriction Fragment Length
sputum

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