Article Figures & Data
Figures
- Fig. 1.
PFGE of XbaI-digested genomic DNAs from 17 nonrepetitive blaIMP-8-positive K. pneumoniae isolates. (A) Lane M, bacteriophage lambda DNA concatemers (Gibco BRL), which served as molecular size markers; lanes 1 to 10, pattern A, isolates 99a300, 99c025, 99c196, 99c893, 99e843, 00m393, 00m749, 00f195, 2907/00, and 00j910. (B) Lane M, bacteriophage lambda DNA concatemers; lane 1, pattern A, isolate 00m578; lanes 2 to 4, pattern C, isolates 00m869, 3396/00, and 00t801; lane 5, pattern D, isolate 00d401; lane 6, pattern E, isolate 99w853; lane 7, pattern B, isolate 3599/00. Isolates 00f195 and 00d401 were from the same patient.
Tables
- Table 1.
Origins of blaIMP-8-containingK. pneumoniae isolates and clinical characteristics of 16 patients with these isolates
Patient no. Age (yr)/sexa Diseaseb Isolate Collection date (day/mo/yr) Wardc Sourced Type of infection Previous therapye Outcome 1 79/M CAD, DM 99w853 23/7/99 SICU Urine Colonization None Death 2 70/M Laryngeal cancer 99a300 1/9/99 SICU Sputum Colonization CAZ Death 99b203 11/9/99 MICU Sputum Colonization 3 73/M PPU, COPD 99c025 20/9/99 SICU Sputum Colonization None Recovered 4 35/F Placenta percreta complicated by perforation of urinary bladder 99c196 22/9/99 SICU Wound Wound infection None Recovered 99e790 23/10/99 Surgical ward Wound Wound infection 5 65/M Traumatic ICH 99c515 27/9/99 SICU Sputum Colonization None Recovered 99c893 30/9/99 SICU Wound Wound infection 6 79/M PPU, DM 00e843 25/10/99 SICU Wound Wound infection CTX, ATM Death 7 47/M Traumatic ICH, DM, liver cirrhosis 3396/00 1/1/00 SICU Blood Bactermia None Death 3397/00 1/1/00 SICU Blood Bacteremia 8 69/F Traumatic ICH, multiple fractures 00k621 3/1/00 SICU Wound Wound infection None Recovered 00k622 3/1/00 SICU Wound Wound infection 3599/00 4/1/00 SICU Blood Bacteremia 3606/00 4/1/00 SICU Blood Bacteremia 00k908 5/1/00 SICU Wound Wound infection 001060 7/1/00 SICU Wound Wound infection 001330 10/1/00 SICU Wound Wound infection 001545 12/1/00 SICU Wound Wound infection 001650 13/1/00 SICU Wound Wound infection 9 68/M Traumatic multiple fractures 00m393 26/1/00 Surgical ward Sputum Colonization None Death 00p177 24/2/00 SICU Sputum Colonization 00p251 25/2/00 SICU Sputum Colonization 00q795 13/3/00 SICU Wound Wound infection 00r091 17/3/00 SICU Sputum Colonization 00r134 17/3/00 SICU Sputum Colonization 00r149 17/3/00 SICU Wound Wound infection 10 73/F Urinary tract cancer 00m749 26/1/00 SICU Sputum Colonization None Recovered 11 69/F PPU, liver cirrhosis 00m869 27/1/00 SICU Wound Wound infection CTX, ATM Death 00n070 31/1/00 SICU Wound Wound infection 00o044 11/2/00 SICU Wound Wound infection 00o227 24/2/00 SICU Wound Wound infection 12 71/M Lung cancer 00t801 17/4/00 SICU Wound Wound infection None Death 13 73/M CAD, DM 00d401 2/8/00 MICU Sputum Colonization CAZ Death 00f195 22/8/00 MICU Sputum Colonization 14 95/F Parkinsonism, COPD 00h908 20/9/00 MICU CVC tip Colonization CAZ, ATM Death 2907/00 23/9/00 MICU Blood Catheter-associated bacteremia MEM 15 76/F Cholangitis, cholelithiasis 00j910 12/10/00 Surgical ward Wound Wound infection None Recovered 00j911 12/10/00 Surgical ward Wound Wound infection 16 76/F CAD, congestive heart failure 00m578 13/11/00 MICU CVC tip Colonization None Recovered ↵a M, male; F, female.
↵b CAD, coronary artery disease; COPD, chronic obstructive pulmonary disease; DM, diabetes mellitus; ICH, intracranial hemorrhage; PPU, perforated peptic ulcer.
↵c MICU, medical intensive care unit; SICU, surgical intensive care unit.
↵d CVC, central venous catheter.
↵e Only broad-spectrum β-lactams are listed. ATM, aztreonam; CAZ, ceftazidime; CTX, cefotaxime; MEM, meropenem.
- Table 2.
Antimicrobial susceptibilities and PFGE patterns of 17 nonrepetitive K. pneumoniae isolates and their transconjugants
Genotypes of β-lactamases, isolate and transconjugant MIC (μg/ml)a Non-β-lactam antibioticsb to which resistance is shown PFGE pattern CAZ CTX FOX ATM IPM MEM Isolate IMP-8 + SHV-12 + TEM1 99a300 >256 64 >256 >256 0.5 0.25 CHL, SXT, GEN, TOB, PEF, OFX, CIP A 99c025 >256 16 >256 256 1 0.5 CHL, SXT, GEN, TOB, PEF, OFX, CIP A 99c196 >256 128 >256 >256 1 1 CHL, SXT, GEN, TOB, PEF, OFX, CIP A 99c893 256 32 >256 >256 1 0.5 CHL, SXT, GEN, TOB, PEF, OFX, CIP A 00m393 >256 256 >256 >256 0.5 0.25 CHL, SXT, GEN, TOB, PEF, OFX, CIP A 00m749 >256 64 >256 >256 0.5 0.25 CHL, SXT, GEN, TOB, PEF, OFX, CIP A 2907/00 >256 32 >256 64 0.5 0.25 CHL, SXT, GEN, TOB, PEF, OFX, CIP A 00j910 >256 128 >256 >256 1 0.5 CHL, SXT, GEN, TOB, PEF, OFX, CIP A 00m578 >256 64 >256 >256 4 1 CHL, SXT, GEN, TOB, PEF, OFX, CIP A 99e843 >256 128 >256 128 1 0.5 CHL, SXT, GEN, TOB A 00f195 >256 64 >256 64 0.5 0.25 CHL, SXT, GEN, TOB A 3599/00 >256 128 >256 >256 2 0.5 CHL, SXT, GEN, TOB, PEF, OFX, CIP B 00m869 >256 128 >256 >256 >256 16 CHL, SXT, GEN, TOB, PEF, OFX, CIP C 00d401 >256 >256 >256 >256 >256 8 CHL, SXT, GEN, TOB D IMP-8 + SHV-11 + TEM1 99w853 >256 64 >256 0.25 2 0.5 CHL, SXT, GEN, TOB, AMK, PEF, OFX, CIP E 3396/00 >256 32 >256 0.06 0.25 0.25 CHL, SXT, TOB, AMK, PEF, OFX, CIP C 00t801 >256 32 >256 0.25 0.5 0.5 CHL, SXT, TOB, AMK, PEF, OFX, CIP C Transconjugant IMP-8 + SHV-12 + TEM1 99a300 256 64 >256 16 0.5 0.25 CHL, SXT, GEN, TOB 99c025 >256 16 >256 32 0.5 0.25 CHL, SXT, GEN, TOB 99c196 256 32 256 16 1 0.25 CHL, SXT, GEN, TOB 99c893 128 16 >256 32 0.5 0.25 CHL, SXT, GEN, TOB 00m393 128 16 128 8 0.25 0.13 CHL, SXT, GEN 00m749 256 64 256 16 0.25 0.25 CHL, SXT, GEN, TOB 2907/00 >256 32 256 16 1 0.25 CHL, SXT, GEN, TOB 00m578 256 16 >256 32 0.5 0.25 CHL, SXT, GEN, TOB 99e843 256 64 >256 16 0.5 0.25 CHL, SXT, GEN, TOB 00f195 128 32 128 8 0.5 0.25 CHL, SXT, GEN, TOB 00d401 128 32 256 16 0.5 0.25 CHL, SXT, GEN, TOB IMP-8 + TEM-1 00m869 256 32 >256 0.13 0.5 0.25 SXT, GEN, TOB 99w853 256 64 >256 0.06 0.5 0.5 CHL, SXT, GEN, TOB
