Article Figures & Data
Figures
- Fig. 1.
Alignment of nucleotide sequences of theBartonella rpoB genes amplified by PCR. The primers used were 1400F and 2300R. Homologies are indicated by dots. 1, B. alsatica; 2, B. bacilliformis; 3, B. berkhoffii; 4, B. clarridgeiae; 5, B. doshiae; 6, B. elizabethae; 7, B. grahamii; 8, B. henselae Houston; 9, B. henselae Marseille; 10, B. quintana; 11, B. taylorii; 12, B. tribocorum; 13, B. vinsonii.
- Fig. 2.
Similarity values between rpoB sequences of various Bartonella species. Values were deduced from the data presented in Fig. 1 by comparison of 825 nucleotides.BhensH, B. henselae Houston; BhensM, B. henselaeMarseille; the remaining abbreviations across the top correspond to the species on the left, from top to bottom, respectively.
- Fig. 3.
Restriction profiles obtained after digestion of a portion of the rpoB gene with ApoI. Ethidium bromide-stained agarose gels of ApoI restriction endonuclease digests of DNA amplified by using primers 1400F and 2300R are shown. Lane A, molecular mass markers (marker IV; Boehringer).Ba, B. alsatica; Bba, B. bacilliformis; Bbe, B. berkhoffii; Bc, B; clarridgeiae; Bd, B. doshiae; Be, B. elizabethae; Bg, B. grahamii; Bh1, B. henselae Houston; Bh2, B. henselae Marseille;Bq, B. quintana; Bta, B. taylorii; Btr, B. tribocorum; Bv, B. vinsonii. Numbers on the left are in base pairs.
- Fig. 4.
ApoI digestion profiles of rpoBamplicons from either B. henselae or B. quintana. Ethidium bromide-stained agarose gels of ApoI restriction endonuclease digests of DNA amplified by using primers 1400F and 2300R are shown. Lane A, molecular mass (marker IV; Boehringer); lanes 1 to 5, DNA extracts from blood of patients infected with B. quintana; lanes 6 to 10, DNA extracts from lymph node or pus aspirate samples from patients suspected of having cat scratch disease and identified as B. henselae-positive samples. Numbers on the left are in base pairs.
Tables
- Table 1.
Bacterial strains used in this study
Species Strain Source (reference)a GenBank accession no. B. alsatica IBS 382T Gift from Pr. PIÉMONT, Strasbourg, France AF165987 B. bacilliformis KC583 ATCC 35685 AF195988 B. berkhoffii NCSU 93-CO1 ATCC 51672 AF165989 B. clarridgeiae Houston-2 cat ATCC 51734 AF165990 B. doshiae R18T NCTC 12862 AF165991 B. elizabethae F9251T ATCC 49927 AF165992 B. henselae Houston-1T ATCC 49882 AF171070 B. henselae Serotype Marseille Endocarditis isolate (8) AF171071 B. grahamii V2T NCTC 12860 AF165993 B. quintana FullerT ATCC VR-358 AF165994 B. taylorii M6T NTCC 12861 AF165995 B. tribocorum CIP 105476T Gift from Pr. PIEMONT, Strasbourg, France AF165996 B. vinsonii BakerT ATCC VR-152 AF165997 ↵a ATCC, American Type Culture Collection, Manassas, Va.; NCTC, National Collection of Type Cultures, Central Public Health Laboratory, London, United Kingdom.
- Table 3.
Endonucleases available for identification ofBartonella group 2 species by PCR-RFLP analysisa
Group 2 species Sizes of fragments (bp) after digestion with: AciI AluI Hin6I MaeII MnlI B. bacilliformis 72, 750 9, 69, 141, 255, 348 94, 218, 510 33, 55, 57, 99, 120, 158, 300 139, 309, 374 B. grahamii 105, 285, 432 2, 36, 39, 54, 63, 118, 154, 158, 198 23, 78, 88, 200, 433 23, 78, 88, 200, 433 164, 658 ↵a Computer analysis was performed from the same partial rpoB gene sequences shown in Fig. 2.
- Table 4.
Endonucleases available for identification ofBartonella group 3 species by PCR-RFLP analysisa
Group 3 species Sizes of fragments (bp) after digestion with: AflIII BsaA1 FnuDII HhaI Hin6I MaeII B. alsatica 822 165, 657 2, 116, 191, 201, 312 154, 160, 198, 310 154, 158, 198, 312 166, 198, 200, 258 B. henselae Houston 57, 765 54, 111, 657 2, 85, 116, 189, 201, 229 203, 205, 314 203, 307, 312 55, 111, 198, 223, 235 B. henselae Marseille 57, 314, 451 54, 111, 342, 315 2, 85, 116, 189, 430 305, 517 307, 515 55, 91, 111, 144, 198, 223 ↵a See footnote a of Table 3.
- Table 5.
Endonucleases available for identification ofBartonella group 4 species by PCR-RFLP analysisa
Group 4 species Sizes of fragments (bp) after digestion with: AluI HhaI Hin6I MaeII MnlI B. berkhoffii 249, 573 105, 154, 160, 403 107, 154, 158, 403 23, 166, 200, 433 10, 812 B. clarridgeiae 6, 69, 141, 255, 351 8, 154, 660 8, 156, 658 8, 23, 57, 88, 150, 196, 300 40, 384, 398 B. doshiae 13, 75, 105, 122, 150, 357 247, 261, 314 249, 261, 312 8, 18, 21, 42, 78, 88, 150, 417 27, 52, 139, 154, 450 B. elizabethae 78, 105, 639 2, 8, 44, 61, 93, 97, 160, 357 2, 8, 44, 61, 95, 97, 158, 357 223, 599 75, 164, 209, 374 B. taylorii 6, 141, 249, 426 8, 52, 146, 148, 154, 154, 160 8, 52, 146, 148, 154, 156, 158 23, 200, 599 398, 424 B. tribocorum 24, 105, 123, 570 5, 58, 247, 512 5, 58, 249, 510 23, 166, 198, 200, 235 154, 658 B. vinsonii 102, 147, 573 2, 105, 144, 154, 160, 257 2, 107, 144, 154, 158, 257 166, 223, 433 52, 770 ↵a See footnote a of Table 3.
- Table 6.
Identification of Bartonella from clinical samples
Clinical material No. of samples No. of samples positive by: Identification (no. of isolates) by: ITS PCR rpoB PCR ITS and 16S rRNA sequencing rpoB PCR-RFLP analysis and rpoBsequencing Lymph node or pus aspirate 94 21 21 B. henselae (21) B. henselaea (21) Blood of patients 10 10 10 B. quintana (10) B. quintana(10) Cat blood 14 14 14 B. clarridgeiae (4),B. henselae (10) B. clarridgeiae (4), B. henselae (10) ↵a DNAs from 5 to 21 samples were positive for Bartonella.
