Article Figures & Data
Figures
- Fig. 1.
PCR-RFLP analysis of 16S rRNA products of B. cepacia complex bacteria and related organisms. Lanes: M, molecular size markers (the sizes [in base pairs] of appropriate bands are indicated on the left); I, II, III, IV, V, VI, and VII, RFLP profiles for genomovars I, II, III, IV, V, VI, and VII, respectively, of the B. cepacia complex; Pan, RFLP profile forPandoreae spp.; Bf, RFLP profile forfungorum.
Tables
- Table 1.
Characteristics of B. cepacia complex and phenotypically similar organismsa
Test or specimen source % of strains positive for test specimen or source Genomovar I Genomovar II Genomovar III Genomovar IV Genomovar V Genomovar VI Genomovar VII B. gladioli Pandoraea species R. pickettii Tests Oxidationbof: Glucose 100 100 95 (96) 100 100 100 100 100 11 (89) 100 Maltose 39 (70) 98 (99) 78 (86) 93 97 (100) 100 100 0 0 92 Lactose 61 (91) 100 79 (88) 93 97 (100) 100 100 0 0 92 Xylose 87 (100) 98 (99) 88 (92) 44 (78) 75 (86) 100 100 96 0 83 (92) Sucrose 87 (91) 0 88 (91) 0 94 (97) 0 94 0 0 0 Adonitol 70 (78) 91 (92) 79 (87) 78 (96) 0 100 100 93 (96) 0 0 Lysine decarboxylase 100 53 99 100 100 0 100 0 0 0 Ornithine decarboxylase 30 0 71 100 0 0 0 0 0 0 Growth at 42°C 43 100 84 0 100 100 22 4 89 83 PNPGc or ONPG 100 98 99 0 100 100 100 100 0 0 Oxidased 100 100 100 100 100 100 100 0 67 100 Nitrate reductionc 4 94 31 4 47 100 67 33 11 17 Gelatin liquefactionc 74 2 55 93 0 0 94 70 0 33 Esculin hydrolysisc 56 2 33 0 0 0 56 11 0 0 Growth on MacConkey agar 83 96 84 93 83 100 100 96 100 50 Pigment brown 4 2 14 0 0 0 6 33 0 0 Pigment yellow 78 2 3 0 0 0 0 44 0 0 Alpha hemolysis 9 1 9 0 36 0 83 22 0 0 Beta hemolysis 9 0 3 0 36 0 83 22 0 0 BCSA 100 100 100 100 100 100 100 18 100 100 API 20 NEe 96 94 86 4 83 100 100 70 0 0 BCESM 0 0 63 0 3 0 0 0 0 0 Specimen sources CF 26 88 76 56 56 100 22 82 100 75 Non-CF clinical 30 6 20 33 19 0 0 11 0 25 Environmental 44 6 4 11 25 0 78 7 0 0 ↵a The number of strains positively identified as being of a particular genomovar or species are as follows: for genomovar I, 23; for genomovar II (B. multivorans), 109; for genomovar III, 139; for genomovar IV (B. stabilis), 27; for genomovar V (B. vietnamiensis), 36; for genomovar VI, 9; for genomovar VII (B. ambifaria), 18; for B. gladioli, 27; forPandoraea species, 9; and for R. pickettii, 12.
↵b Oxidation test results were recorded after 3 days of incubation (data in parentheses were recorded after 7 days of incubation).
↵c Results presented are from API 20 NE strip test.
↵d In tests using the Pathotech cytochrome oxidase strip, the slow, weak reaction occurred in 10 to 30 s and the fast, strong reaction occurred in less than 10 s. OnlyR. pickettii displayed a fast, strong reaction.
↵e For B. cepacia complex strains, results represent very good or excellent identification (≥99.0%), although API database admits that any given strain may be B. gladioli.
