DOI: 10.1128/JCM.39.3.1172-1174.2001
Article Figures & Data
Tables
- Table 1.
Analysis of clinical isolates by trehalose-sucrose assimilation test based on Rosco diagnostic tablets
Organism No. of isolates No. of isolates testing positive Trehalose Sucrose 4 h 24 h 4 h 24 h C. glabrata 180 180 180 0 0 C. albicans 84 0 0 21 25 C. tropicalis 62 8 21 53 53 C. kefyr 38 0 0 33 37 C. krusei 22 0 0 0 0 C. parapsilosis 19 0 0 0 1 S. cerevisiae 11 0 1 10 10 C. sake 9 0 1 1 3 C. famata 7 0 0 0 1 C. lusitaniae 4 0 0 2 3 C. guilliermondii 2 0 0 1 1 C. sphaerica 1 0 0 1 1 T. cutaneum 1 0 0 0 0 - Table 2.
Analysis of reference strains by trehalose-sucrose assimilation test based on Rosco diagnostic tablets
Organism Test result Trehalose Sucrose 4 h 24 h 4 h 24 h C. albicans ATCC 26278 − − − − C. parapsilosis ATCC 22019 − − − − C. glabrata ATCC 66032 + + − − C. krusei ATCC 6258 − − − − C. conglobata CBS 2018 − − − − C. conglobata CBS 2019 − − − − C. zeylanoides CBS 619 − − − − C. zeylanoides CBS 947 − − − − P. farinosa CBS 185 − − − − P. farinosa CBS 2001 − − − − P. wickerhamii IP 1202-79 − − − − - Table 3.
Use of trehalose-sucrose assimilation test to identify yeast isolates obtained from patients receiving antifungal treatment at time of sampling
Organism No. of isolates No. of isolates testing positive Trehalose Sucrose 4 h 24 h 4 h 24 h C. albicans 9 0 0 0 0 C. glabrata 10 10 10 0 0 C. kefyr 2 0 0 2 2 C. tropicalis 1 0 0 1 1 C. krusei 1 0 0 0 0 S. cerevisiae 1 0 0 1 1 - Table 4.
Sensitivity, specificity, and cost of different tests for rapid identification of C. glabrata
Method (reference) Inoculum Incubation Sensitivity (%) Specificity (%) Cost ($) Hardy trehalose fermentation (1) Four to five colonies 24 h, 42°C 96 100 1.10 Mayo Clinic trehalose assimilation (1; Stockman and Robertsa) Heavy; not adjusted 1 h, 35°C 96.6 74.1 0.05 Remel rapid trehalose assimilation (1) Cloudy suspension 3 h, 42°C 91.5 96.3 1.59 Remel yeast fermentation, modified (1, 7) No. 3–4 McFarland standard 24 h, 42°C 95–97.8 89–95.8 4.15 Rosco diagnostic tablets (current study) No. 2 McFarland standard 4 h, 37°C 100 100 0.70 Trehalase-generated glucose (8) One colony/50 μl 3 h, 37°C 98.8 99.1 0.30 ↵a L. Stockman and G. Roberts, Abstr. 85th Annu. Meet. Am. Soc. Microbiol. 1985, abstr. F-80, p. 377, 1985.
