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Mycology

Rapid Identification of Candida glabrata Based on Trehalose and Sucrose Assimilation Using Rosco Diagnostic Tablets

José Lopez, Frédéric Dalle, Pierre Mantelin, Philippe Moiroux, Anne-Charlotte Nierlich, Agnès Pacot, Bernadette Cuisenier, Odile Vagner, Alain Bonnin
José Lopez
Laboratoire de Parasitologie et Mycologie, Hôpital du Bocage, 21034 Dijon Cedex, France
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Frédéric Dalle
Laboratoire de Parasitologie et Mycologie, Hôpital du Bocage, 21034 Dijon Cedex, France
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Pierre Mantelin
Laboratoire de Parasitologie et Mycologie, Hôpital du Bocage, 21034 Dijon Cedex, France
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Philippe Moiroux
Laboratoire de Parasitologie et Mycologie, Hôpital du Bocage, 21034 Dijon Cedex, France
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Anne-Charlotte Nierlich
Laboratoire de Parasitologie et Mycologie, Hôpital du Bocage, 21034 Dijon Cedex, France
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Agnès Pacot
Laboratoire de Parasitologie et Mycologie, Hôpital du Bocage, 21034 Dijon Cedex, France
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Bernadette Cuisenier
Laboratoire de Parasitologie et Mycologie, Hôpital du Bocage, 21034 Dijon Cedex, France
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Odile Vagner
Laboratoire de Parasitologie et Mycologie, Hôpital du Bocage, 21034 Dijon Cedex, France
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Alain Bonnin
Laboratoire de Parasitologie et Mycologie, Hôpital du Bocage, 21034 Dijon Cedex, France
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DOI: 10.1128/JCM.39.3.1172-1174.2001
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Tables

  • Table 1.

    Analysis of clinical isolates by trehalose-sucrose assimilation test based on Rosco diagnostic tablets

    OrganismNo. of isolatesNo. of isolates testing positive
    TrehaloseSucrose
    4 h24 h4 h24 h
    C. glabrata18018018000
    C. albicans84002125
    C. tropicalis628215353
    C. kefyr38003337
    C. krusei220000
    C. parapsilosis190001
    S. cerevisiae11011010
    C. sake90113
    C. famata70001
    C. lusitaniae40023
    C. guilliermondii20011
    C. sphaerica10011
    T. cutaneum10000
  • Table 2.

    Analysis of reference strains by trehalose-sucrose assimilation test based on Rosco diagnostic tablets

    OrganismTest result
    TrehaloseSucrose
    4 h24 h4 h24 h
    C. albicans ATCC 26278−−−−
    C. parapsilosis ATCC 22019−−−−
    C. glabrata ATCC 66032++−−
    C. krusei ATCC 6258−−−−
    C. conglobata CBS 2018−−−−
    C. conglobata CBS 2019−−−−
    C. zeylanoides CBS 619−−−−
    C. zeylanoides CBS 947−−−−
    P. farinosa CBS 185−−−−
    P. farinosa CBS 2001−−−−
    P. wickerhamii IP 1202-79−−−−
  • Table 3.

    Use of trehalose-sucrose assimilation test to identify yeast isolates obtained from patients receiving antifungal treatment at time of sampling

    OrganismNo. of isolatesNo. of isolates testing positive
    TrehaloseSucrose
    4 h24 h4 h24 h
    C. albicans90000
    C. glabrata10101000
    C. kefyr20022
    C. tropicalis10011
    C. krusei10000
    S. cerevisiae10011
  • Table 4.

    Sensitivity, specificity, and cost of different tests for rapid identification of C. glabrata

    Method (reference)InoculumIncubationSensitivity (%)Specificity (%)Cost ($)
    Hardy trehalose fermentation (1)Four to five colonies24 h, 42°C961001.10
    Mayo Clinic trehalose assimilation (1; Stockman and Robertsa)Heavy; not adjusted1 h, 35°C96.674.10.05
    Remel rapid trehalose assimilation (1)Cloudy suspension3 h, 42°C91.596.31.59
    Remel yeast fermentation, modified (1, 7)No. 3–4 McFarland standard24 h, 42°C95–97.889–95.84.15
    Rosco diagnostic tablets (current study)No. 2 McFarland standard4 h, 37°C1001000.70
    Trehalase-generated glucose (8)One colony/50 μl3 h, 37°C98.899.10.30
    • ↵a L. Stockman and G. Roberts, Abstr. 85th Annu. Meet. Am. Soc. Microbiol. 1985, abstr. F-80, p. 377, 1985.

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Rapid Identification of Candida glabrata Based on Trehalose and Sucrose Assimilation Using Rosco Diagnostic Tablets
José Lopez, Frédéric Dalle, Pierre Mantelin, Philippe Moiroux, Anne-Charlotte Nierlich, Agnès Pacot, Bernadette Cuisenier, Odile Vagner, Alain Bonnin
Journal of Clinical Microbiology Mar 2001, 39 (3) 1172-1174; DOI: 10.1128/JCM.39.3.1172-1174.2001

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Rapid Identification of Candida glabrata Based on Trehalose and Sucrose Assimilation Using Rosco Diagnostic Tablets
José Lopez, Frédéric Dalle, Pierre Mantelin, Philippe Moiroux, Anne-Charlotte Nierlich, Agnès Pacot, Bernadette Cuisenier, Odile Vagner, Alain Bonnin
Journal of Clinical Microbiology Mar 2001, 39 (3) 1172-1174; DOI: 10.1128/JCM.39.3.1172-1174.2001
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KEYWORDS

Candida
Sucrose
Trehalose

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