Article Figures & Data
Figures
- Fig. 1.
Representative fivefold dilution series of HIV-1 group M and group O isolates. (a) Group M subtypes A, B, C, and D. (b) Group M subtypes CRF-AE, F, and G and group O. Log10 RNA copies per milliliter are plotted versus the dilution series order, whereby numbers 1 through 7 correspond to neat, 1:5, 1:25, 1:125, 1:625, 1:3,125, and 1:15,625 dilutions, respectively. Quantitative values and linear regression trend lines for each of the three commercial viral load tests are shown as follows: solid circle and solid line, LCx HIV assay; open circle and dotted line, MONITOR v1.5 test; and triangle and dotted-dashed line, bDNA v3.0 assay.
- Fig. 2.
Comparison of HIV-1 group M virus isolate dilution panel members quantified by the LCx HIV assay and the MONITOR v1.5 test. Group (Grp) O dilution panel members were not quantified by MONITOR v1.5 but are shown for comparison. The lower limit of quantification is shown by broken lines at 1.7 log10 RNA copies/ml (50 copies/ml).
- Fig. 3.
Comparison of HIV-1 group M and O virus isolate dilution panel members quantified by the LCx HIV assay and the bDNA v3.0 assay. The lower limit of quantification is shown by broken lines at 1.7 log10 RNA copies/ml (50 copies/ml).
- Fig. 4.
Comparison of HIV-1 group M virus isolate dilution panel members quantified by the MONITOR v1.5 test and the bDNA v3.0 assay. Group O dilution panel members were not quantified by MONITOR v1.5 but are shown for comparison. The lower limit of quantification is shown by broken lines at 1.7 log10 RNA copies/ml (50 copies/ml).
Tables
- Table 1.
Characteristics of the viral-isolate panel
Group Subtypea Isolate Country M A UG273 Uganda CRF-AG (IbNG) (A/G/A) DJ258 Djibouti CRF-AG (IbNG) (A/G/A) DJ263 Djibouti B US1 United States B US2 United States B US3 United States B US4 United States B/A/B CM237 Thailand B BK132 Thailand B BZ167 Brazil C ZAM18 Zambia C UG268 Uganda C ETH2220 Ethiopia C SE364 Senegal C SM145 Somalia D SE365 Senegal D UG270 Uganda D UG274 Uganda CRF-AE (A/A/E)b CM235 Thailand CRF-AE (A/A/E) CM238 Thailand CRF-AE (A/A/E) CM240 Thailand CRF-AE (A/A/E) CM243 Thailand CRF-AE (A/A/E) POC30506 Thailand CRF-AE (A/A/E) ID12 Indonesia CRF-AE (A/A/E) ID17 Indonesia CRF-AE (A/A/E) NP1465 Thailand F BZ126 Brazil F BZ162 Brazil F BZ163 Brazil G HH8793 Kenya A UG95-327 Uganda A UG95-422 Uganda B TH1600 Thailand C UG95-175 Uganda D UG95-306 Uganda F BR57 Brazil A/G/G IVCO3671 Ivory Coast O 3012 Spain 08692A United States - Table 2.
Nucleotide mismatches at primer and probe binding sites for the LCx HIV assay and the MONITOR v1.5 test
Assay or test Primer or probe Mean (range) no. of nucleotide mismatches for: Group M subtype Group O A B C D CRF-AE F G LCx HIV HIV-1 forward 0 0.1 (0–1) 1.0 (0–2) 0 1 (1) 1 (1) 1 (1) 4 (4) HIV-1 probe 0 0 0.2 (0–1) 0 0.1 (0–1) 0 0 0 HIV-1 reverse 1 (0–3) 0.4 (0–2) 0.2 (0–1) 0 0 1 (1) 0 1 (1) Total 1 (0–3) 0.6 (0–2) 1.3 (0–3) 0 1.1 (1–2) 2 (2) 1 (1) 5 (5) MONITOR v1.5 SK145 forward 3.3 (3–4) 0 0 0 3 (3) 0.5 (0–1) 2 (2) 9.5 (9–10) SK102 probe 2 (2) 0.4 (0–1) 2.2 (2–3) 0.2 (0–1) 0.9 (0–1) 2.5 (2–3) 4 (4) 7.5 (7–8) SKCC1B reverse 0.7 (0–1) 0.3 (0–1) 0.3 (0–1) 1.2 (1–2) 0 2 (2) 2 (2) 5 (5) Total 6 (6) 0.7 (0–1) 2.5 (2–4) 1.5 (1–2) 3.9 (3–4) 5 (4–6) 8 (8) 22 (21–23)
