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Journal of Clinical Microbiology
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Mycology

Inoculum Standardization for Antifungal Susceptibility Testing of Filamentous Fungi Pathogenic for Humans

Eva Petrikkou, Juan L. Rodrı́guez-Tudela, Manuel Cuenca-Estrella, Alicia Gómez, Ana Molleja, Emilia Mellado
Eva Petrikkou
Servicio de Micologia, Centro Nacional de Microbiologia, Instituto de Salud Carlos III, 28220 Majadahonda, Spain
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Juan L. Rodrı́guez-Tudela
Servicio de Micologia, Centro Nacional de Microbiologia, Instituto de Salud Carlos III, 28220 Majadahonda, Spain
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Manuel Cuenca-Estrella
Servicio de Micologia, Centro Nacional de Microbiologia, Instituto de Salud Carlos III, 28220 Majadahonda, Spain
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Alicia Gómez
Servicio de Micologia, Centro Nacional de Microbiologia, Instituto de Salud Carlos III, 28220 Majadahonda, Spain
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Ana Molleja
Servicio de Micologia, Centro Nacional de Microbiologia, Instituto de Salud Carlos III, 28220 Majadahonda, Spain
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Emilia Mellado
Servicio de Micologia, Centro Nacional de Microbiologia, Instituto de Salud Carlos III, 28220 Majadahonda, Spain
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DOI: 10.1128/JCM.39.4.1345-1347.2001
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ABSTRACT

Two methods of inoculum preparation for filamentous fungi were compared: counting with a hematocytometer and spectrophotometric adjustment. One hundred eighty-two filamentous fungi pathogenic for humans were used. Colony counts were done for all inoculum preparations. The agreement between the hematocytometer counts and the colony counts (CFU per milliliter) was 97.2%. The reproducibility between the hematocytometer counts and the colony counts by means of an intraclass correlation coefficient was 0.70. Pearson's correlation index for hematocytometer counts versus colony counts was 0.56, whereas that for optical density versus colony counts was 0.008. Both methods can be used for inoculum size adjustment. However, the use of the spectrophotometric method requires that each species be standardized separately.

  • Copyright © 2001 American Society for Microbiology
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Inoculum Standardization for Antifungal Susceptibility Testing of Filamentous Fungi Pathogenic for Humans
Eva Petrikkou, Juan L. Rodrı́guez-Tudela, Manuel Cuenca-Estrella, Alicia Gómez, Ana Molleja, Emilia Mellado
Journal of Clinical Microbiology Apr 2001, 39 (4) 1345-1347; DOI: 10.1128/JCM.39.4.1345-1347.2001

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Inoculum Standardization for Antifungal Susceptibility Testing of Filamentous Fungi Pathogenic for Humans
Eva Petrikkou, Juan L. Rodrı́guez-Tudela, Manuel Cuenca-Estrella, Alicia Gómez, Ana Molleja, Emilia Mellado
Journal of Clinical Microbiology Apr 2001, 39 (4) 1345-1347; DOI: 10.1128/JCM.39.4.1345-1347.2001
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KEYWORDS

Microbial Sensitivity Tests
Mitosporic Fungi
mycoses

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