Genotyping Encephalitozoon hellemIsolates by Analysis of the Polar Tube Protein Gene

DOI: 10.1128/JCM.39.6.2191-2196.2001

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Fig. 1.
Sequence differences in the rRNA ITS among E. hellem genotypes. Dots denote sequence identity to genotype 1A, and dashes depict nucleotide deletions. Genotypes 1A, 1B, and 1C = genotype 1 of Mathis et al.; genotype 2A = genotype 2 of Mathis et al.; genotype 2C = genotype 3 of Mathis et al. (21).
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Fig. 2.
Sequence diversity among E. hellem genotypes in the PTP gene. Dots denote sequence identity to genotype 1A (AF044915 ), and dashes depict nucleotide deletions. The repeat region is underlined, and the primer sequences used in direct genotyping PCR are double underlined. The numbers at the ends of lines are nucleotide positions in AF044915 .
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Fig. 3.
Genotyping E. hellem isolates by PCR analysis of the PTP gene. Lanes 1 and 9, 100-bp ladders; lanes 2 and 5, genotype 1B; lanes 3 and 4, genotype 1A; lane 6, genotype 2B; and lanes 7 and 8, genotype 1C.

Table 1.

Human E. hellem isolates used in this study and genotyping results from analyses of ITS, SSU rRNA, and PTP genes

Isolate	Origin^a	Sex/age (yr)^b	Isolation (mo/yr)	Country/state	ITS genotype^c	SSU rRNA genotype^d	PTP genotype	Reference(s)
CDC:V278	Urine	M	11/92	Puerto Rico	1A	1A	1A	37
CDC:V242	Urine	M/26^*	01/92	USA/Ga.	1A	1A	1A	31, 37
CDC:V274	BAL	M/26^*	10/92	USA/Ga.	1A	1A	1A	31, 37
Le	Conjunctiva	M/46	12/89	USA/N.Y.	1A	1A	1A	7, 13
CDC:V257	Urine	F/35^{^†}	06/92	USA/N.Y.	1A	1A	1A	31, 37
CDC:V258	Sputum	F/35^{^†}	06/92	USA/N.Y.	1A	1A	1A	31, 37
CDC:V281	Nasal	F/35^{^†}	12/92	USA/N.Y.	1A	1A	1A	31, 37
Wo	Conjunctiva	M/37	1994	USA/Mass.	1A	1A	1A	7, 9
Ye	Cornea	M/29	12/89	USA/Tex.	1A	1A	1A	7, 40
Ly	Cornea	M/30	1990	USA/Ohio	1A	1A	1A	Unpublished data
He	Conjunctiva	M/33	12/89	USA/N.Y.	1A	1A	1B	7, 13
CDC:V213	Urine	M/30	02/91	USA/Ga.	1A	1A	1B	31, 37
CDC:SP-1	BAL	M/41	1996	Spain	1A	1A	1B	22
LEPV-2-93	BAL	M/27	10/93	Italy	1A	1A	1B	30, 37
PV-3-93	BAL	M/30	12/93	Italy	1A	1A	1B	29, 37
PV-8-95	Sputum	F/34	03/95	Italy	1A	1C	1C	14, 37
VRPV-1-93	Urine	M/33	09/93	Italy	1A	1C	1C	14, 37
PV-5-95	BAL	M/38	01/95	Italy	1A	1C	1C	29, 37
PV-11-98	Nasal	M/34	01/98	Italy	1A	1C	1C	14, 37
PV-10-97	Ocular	M/12^●	01/97	Italy	1A	1C	1C	14, 37
PV-10-97EI	Sputum	M/12^●	01/97	Italy	1A	1C	1C	14, 37
MIPV-6-95	BAL	F/33	10/95	Italy	1A	1C	1C	14, 37
PV-9-96	BAL	M/38^e	07/96	Italy	1A	1C	1C	28
CDC:V261	Urine	M/49	06/92	Switzerland	2B	2B	2B	37, 38

↵a BAL, bronchoalveolar lavage.
↵b Superscript symbols indicate multiple samples from the same patient with the same symbol.
↵c Genotypes 1A, 1B, and 1C had an identical ITS sequence.
↵d Genotypes 1A and 1B had an identical SSU rRNA sequence.
↵e HIV negative.