Article Figures & Data
Figures
- Fig. 1.
Bronchiole in the lung of a pig inoculated only withM. hyopneumoniae and euthanatized 28 DPI. The bronchiole is lined by normal tall columnar epithelial cells and is surrounded by a prominent cuff of infiltrating lymphocytes.
- Fig. 2.
Bronchiole in the lung of a pig inoculated only with SIV and euthanatized 3 DPI. The bronchiole is lined by a thin layer of attenuated epithelium subsequent to necrosis and sloughing of epithelial cells infected with the virus. A light loose lymphocytic infiltrate surrounds the bronchiole.
- Fig. 3.
Bronchiole in the lung of a pig inoculated with bothM. hyopneumoniae and SIV. The pig was euthanatized 3 DPI with SIV and 24 DPI with M. hyopneumoniae. The bronchiole exhibits the characteristic disruption and attenuation of the epithelial layer and early irregular reactive proliferation subsequent to SIV infection and intense peribronchiolar lymphocyte infiltration induced by both virus and mycoplasma.
Tables
- Table 1.
Experimental design—infection status and number of pigs necropsied on each of 4 postinfection days
Group Infection status (day of inoculationa) No. of pigs necropsied at day: Total no. of pigs necropsied 3 7 14 21 MHYO M. hyopneumoniae (−21) 5 5 5 5 20 SIV SIV (0) 5 5 5 5 20 DUAL SIV (0) and M. hyopneumoniae (−21) 5 5 5 5 20 CONTROL No infection 3 3 3 3 12 ↵a Pigs were 8 weeks old on day 0.
- Table 2.
Summary (group mean ± standard deviation) of clinical observations following challenge with M. hyopneumoniaeand/or SIVa
Group Infection status Rectal temperatureb Coughing scorec Gain per dayd MHYO M. hyopneumoniae 0.05 ± 0.09 A,B 0.10 ± 0.23 A,B 1.45 ± 0.11 A,B SIV SIV 0.37 ± 0.22 B,C 0.27 ± 0.27 B 1.30 ± 0.16 A DUAL SIV and M. hyopneumoniae 0.45 ± 0.22 C 0.78 ± 0.38 C 1.51 ± 0.10 B CONTROL No infection 0.27 ± 0.26 B 0.0 ± 0.0 A 1.92 ± 0.06 C ↵a Within each column, values followed by different letters (A, B, C) are significantly different (P < 0.05).
↵b Proportion of days (out of 7 total) that each pig's rectal temperature was ≥104°F.
↵c Proportion of days (out of 7 total) that each pig was observed coughing.
↵d Average daily weight gain (pounds) of pigs at 21 days post-SIV inoculation.
- Table 3.
Percentage of lung with visible pneumonia lesions as determined by lesion sketches and image analysis for pigs challenged with M. hyopneumoniae and/or SIV
Group Infection status % of lung with visible lesions on necropsy at DPIa: 3 7 14 21 MHYO M. hyopneumoniae 2.1 ± 1.8 A,B 3.6 ± 3.1 A,B 3.3 ± 2.6 A 7.6 ± 7.4 SIV SIV 10.3 ± 7.4 B 8.8 ± 5.8 B,C 3.3 ± 2.5 A 1.1 ± 1.4 DUAL SIV and M. hyopneumoniae 15.0 ± 6.0 B 9.8 ± 3.5 C 11.4 ± 6.3 B 4.6 ± 4.0 CONTROL No infection 0.0 ± 0.0 A 0.0 ± 0.0 A 0.0 ± 0.0 A 0.0 ± 0.0 ↵a Pigs were 8 weeks old on day 0. Within each column, values followed by different letters (A, B, or C) are significantly different (P < 0.05) . Data are means ± standard deviations.
