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Journal of Clinical Microbiology
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Bacteriology

Pasteurellamultocida subsp.multocida and P. multocida subsp.septica Differentiation by PCR Fingerprinting and α-Glucosidase Activity

Sharon Hunt Gerardo, Diane M. Citron, Marina C. Claros, Helen T. Fernandez, Ellie J. C. Goldstein
Sharon Hunt Gerardo
R. M. Alden Research Laboratory, Santa Monica/UCLA Medical Center, Santa Monica, California 90404;
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Diane M. Citron
R. M. Alden Research Laboratory, Santa Monica/UCLA Medical Center, Santa Monica, California 90404;
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Marina C. Claros
Institute of Medical Microbiology, University of Leipzig, Leipzig, Germany; and
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Helen T. Fernandez
R. M. Alden Research Laboratory, Santa Monica/UCLA Medical Center, Santa Monica, California 90404;
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Ellie J. C. Goldstein
R. M. Alden Research Laboratory, Santa Monica/UCLA Medical Center, Santa Monica, California 90404;
University of California School of Medicine, Los Angeles, California 90095
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DOI: 10.1128/JCM.39.7.2558-2564.2001
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ABSTRACT

Pasteurella multocida is composed of three subspecies that are often differentiated by fermentation of sorbitol and dulcitol. We studied 35 dulcitol-negative P. multocida isolates from infected dog and cat bite wounds, 16 of which yielded weak and/or conflicting fermentation reactions in Andrades sorbitol, thus making it difficult to distinguish between the two dulcitol-negative subspecies of P. multocida, i.e.,P. multocida subsp. multocida andP. multocida subsp. septica. All isolates and two control strains were further analyzed using a PCR fingerprinting technique with a single primer (M13 core) and assessed for α-glucosidase (α-Glu) activity. Although the PCR fingerprint patterns and α-Glu activity did not correlate well with the sorbitol fermentation reactions, they did correlate well with each other. All strains identified as P. multocida subsp.septica were positive for α-Glu activity and exhibited the group I PCR fingerprint profile. All strains categorized asP. multocida subsp. multocida displayed either the group II or group III PCR fingerprint profile; 9 of 11 of these isolates were α-Glu negative. These data suggest that both PCR fingerprinting and α-Glu activity provide reliable means for differentiating P. multocida subsp.multocida from P. multocida subsp.septica, particularly in strains that produce weak and/or discrepant sorbitol fermentation reactions.

  • Copyright © 2001 American Society for Microbiology
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Pasteurellamultocida subsp.multocida and P. multocida subsp.septica Differentiation by PCR Fingerprinting and α-Glucosidase Activity
Sharon Hunt Gerardo, Diane M. Citron, Marina C. Claros, Helen T. Fernandez, Ellie J. C. Goldstein
Journal of Clinical Microbiology Jul 2001, 39 (7) 2558-2564; DOI: 10.1128/JCM.39.7.2558-2564.2001

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Pasteurellamultocida subsp.multocida and P. multocida subsp.septica Differentiation by PCR Fingerprinting and α-Glucosidase Activity
Sharon Hunt Gerardo, Diane M. Citron, Marina C. Claros, Helen T. Fernandez, Ellie J. C. Goldstein
Journal of Clinical Microbiology Jul 2001, 39 (7) 2558-2564; DOI: 10.1128/JCM.39.7.2558-2564.2001
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KEYWORDS

Bites and Stings
Pasteurella Infections
Pasteurella multocida
polymerase chain reaction
Wound Infection
alpha-Glucosidases

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