DOI: 10.1128/JCM.39.8.2975-2977.2001
Article Figures & Data
Figures
- Fig. 1.
Agarose gel electrophoresis of PCR-RFLP products showing the cleavage patterns of the 956-bp amplified products. Lane M, molecular standards (100-bp digest).
- Fig. 2.
Comparison of protein sequences of our Ad (this Ad) and Ad9. Identical sequences are boxed.
Tables
- Table 1.
Primer sets for PCR
Forward Reverse Positions Primer Sequence Primer Sequence Ad1F 5′-ATGGCCACCCCCTCGATGCCG-3′ Ad1R 5′-GCAAATGAGCCATAGCATGG-3′ 119–758 Ad2F 5′-TCATTTGACATCCGGGGCGTCCTGGA-3′ Ad2R 5′-CTGTCCACCGCAGAGTTCCACAT-3′ 418–1237 Ad3F 5′-ATGTGGAACTCTGCGGTGGACAG-3′ Ad3R 5′-GGGATGGGGTAGAGCATGTTGGCGGC-3′ 1261–2022 Ad4F 5′-CGATGCTATTGAGAGAGAGG-3′ Ad4R 5′-GTAGGGTTTAAAGCTGGACC-3′ 33–421 Ad5F 5′-TCATGTGGTGTACAAGCCAGG-3′ Ad5R 5′-GCGTGGACTTAAAGGAGTCG-3′ 997–1551 AdnU-S 5′-TTCCCCATGGCNCACAACAC-3′ AdnU-A 5′-GCCTCGATGACGCCGGCGGTG-3′ 1866–2820 - Table 2.
Homology between hexon sequences of new Ad and members of Ad subgenus D
Ad % Homologya HVR1 HVR2 HVR3 HVR4 HVR5 HVR6 HVR7 All HVRs Ad8 15.2 20.0 42.9 50.0 25.0 33.3 41.7 28.9 Ad9 56.3 80.0 85.7 50.0 75.0 40.0 66.7 63.6 Ad15 47.1 40.0 42.9 18.8 52.9 60.0 68.0 49.0 Ad19 40.0 10.0 42.9 43.8 35.0 33.3 58.3 40.6 Ad37 31.6 55.6 40.0 25.0 12.5 55.6 32.0 32.3 Ad48 27.8 16.7 66.7 41.2 21.1 33.3 25.0 29.5 ↵a Percent homology between the indicated HVR of the new Ad and that of each subgenus D Ad.
