Article Figures & Data
Figures
- Fig. 1.
Biprobe peak profiles. (A) Peak profiles obtained with biprobe A. Peak 0 melted at 58°C, peak 1 melted at 52°C, and peak 2 melted at 50°C. (B) Peak profiles obtained with biprobe B. Peak 0 melted at 64°C, peak 1 melted at 60°C, peak 2 melted at 56°C, peak 3 melted at 58°C, peak 4 melted at 53°C, and peak 5 melted at 52°C. (C) Peak profiles obtained with biprobe C. Peak 0 melted at 68°C, peak 1 melted at 60°C, peak 2 melted at 56°C, and peak 3 melted at 54°C. (D) Peak profiles obtained with biprobe D. Peak 0 melted at 60°C, and peak 1 melted at 58°C.
Tables
- Table 1.
Type strains used in the study
Species Strain S. arlettae NCTCa 12413 S. aureus NCTC 8523 S. auricularis NCTC 12101 S. capitis NCTC 11045 S. caprae NCTC 12196 S. chromogenes NCTC 10530 S. cohnii NCTC 11041 S. delphini NCTC 12225 S. epidermidis NCTC 11047 S. equorum NCTC 12414 S. gallinarum NCTC 12195 S. haemolyticus NCTC 11042 S. hominis NCTC 11320 S. hyicus NCTC 10350 S. intermedius NCTC 11048 S. kloosii NCTC 12415 S. lentus NCTC 12102 S. lugdunensis NCTC 12217 S. saprophyticus NCTC 7292 S. sciuri NCTC 12103 S. schleiferi NCTC 12218 S. simulans NCTC 11046 S. warneri NCTC 11044 S. xylosus NCTC 11043 Stomatococcus mucilaginosus NCTC 10663 Micrococcus luteus NCTC 2665 ↵a NCTC, National Collection of Type Cultures (Central Public Health Laboratory, London, England).
- Table 2.
DNA sequences of primers and biprobes
Name of primer or probe Sequence Primers STAR1 5′-GCG GdT CCA TCT ATA AGT GA-3′a STAF1 5′-GGG TGA GTA ACA CGT GGA-3′ STAF2 5′-GGG TGA GTA ACA CGT GGG-3′ STBF1 5′-ATT CGA AGC AAC GCG AAG-3′ STBR1 5′-CCA TGC ACC ACC TGT CAC-3′ Probes CNS Probe A 5′-GGA TAA TAT ATT GAA CCG CA-3′ CNS Probe B 5′-TCC GGT TTC CCG AAG TTG TC-3′ CNS Probe C 5′-GAG CGG TCA AAG GAT GTC AAG-3′ CNS Probe D 5′-CCT CTG ATC CCT CTA GAA ATA-3′ ↵a d, A or G.
- Table 3.
Biprobe identification scheme for CNSa
Organism Biprobe melting peakb obtained with: CNS Probe A CNS Probe B CNS Probe C CNS Probe D S. epidermidis 0 3 3 1 S. warneri 1 1 0 – S. lugdunensis 2 0 0 – S. aureus 2 3 2 – S. intermedius 2 4 0 – S. chromogenes 2 4 3 – S. sciuri 2 5 0 – S. hominis – 1 or 2 1 – S. haemolyticus – 2 2 – S. capitis – 3 3 0 or 1 S. caprae – 3 3 1 S. schleiferi – 4 0 – S. simulans – 4 2 – S. saprophyticus – 4 3 – S. cohnii – 5 2 – - Table 4.
Results of testing of 142 clinical isolates by ID 32 Staph and PCR and by real-time PCR
Organism Total no. of isolates No. of isolates identified by: ID 32 Staph and PCR Real-time PCR 16S rRNA sequencing S. hominis 17 17 17 –b S. sciuri 3 3 3 – S. cohnii 2 2 2 – S. intermedius 2 2 2 – S. simulans 5 5 5 – S. epidermidis 30 26 30 4 S. aureus 11 10 11 1 S. saprophyticus 6 5 6 1 S. warneri 11 13 11 – S. haemolyticus 24 27 24 – S. schleiferi 4 6 4 – S. lugdunensis 10 12 10 – S. capitis 12 14 7 1 S. arlettae 1 0 0 1 S. pasteuri 1 0 0 1 Staphylococcussp. 1 0 0 1 Acinetobacter lwoffii 1 0 0 1 Micrococcus luteus 1 0 0 1 a Number of isolates identified using a combination of all tests and using 16S rRNA sequencing to confirm identity when discrepancies occurred between the ID 32 Staph and PCR and the real-time PCR identifications.
↵b –, not performed.
