Molecular and Epidemiological Analyses of Human Adenovirus Type 7 Strains Isolated from the 1995 Nationwide Outbreak in Japan

DOI: 10.1128/JCM.40.1.140-145.2002

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FIG. 1.
Restriction patterns of viral DNA of Ad7 strains digested with BglI, SacI, BamHI, BglII, HindIII, PstI, PvuII, SmaI, or BstEII. The lane numbers correspond with the codes of restriction pattern by each enzyme listed in Table 3. Molecular sizes are expressed as kilobase pairs.

TABLE 1.

List of Ad7 strains used in this study

Strain code in this study	Origin		No. of strains used	Genome type reported by^a:
Strain code in this study	Place	Year	No. of strains used	Wadell et al., Li et al.	Adrian et al.
Ad7p	United States	1954	1	Ad7p	D1
Ad7a	United States	1958	1	Ad7a	D2
CH/90	Beijing, China	1990	2	Ad7d
NR/87	Nara Prefecture	1987	2
KY/87	Kyoto Prefecture	1987	1
AI/92	Aichi Prefecture	1992	19
HR/95	Hiroshima City	1995	12
AK/95	Akita Prefecture	1995	1
YM/95	Yamanashi Prefecture	1995	4
YK/95	Yokohama City	1995	1
NR/95	Nara Prefecture	1995	8

TABLE 2.

List of primers used in this study

Primer code	Target region	Size of PCR products (bp)	Sequence of primer	Position^a
BH-S	Hexon	788	5′-TACCTCTCCGCCGCAAACAT-3′	1891–1910
BH-A			5′-TCCACCTCAAAAGTCATCTC-3′	2678–2659
BF-S	Fiber	1,255	5′-CACTTACTTGAAATCAGCAATA-3′	4354–4375
BF-A			5′-AGCTGTGCGTGGGGAGAGATTG-3′	5608–5587
BE3-1S	5′ End part of E3	1,146	5′-TCGAGACGCCCAGGCCGAAG-3′	219–238
BE3-1A			5′-AAGCGAAAGTAAAGCAAGCACGAC-3′	1364–1341
BE3-2S	Middle part of E3	1,380	5′-CTGGGTGGTAGCGGGTTTTGTA-3′	1206–1227
BE3-2A			5′-CTCTAGCGAACCCTCCATTATC-3′	2585–2564
BE3-3S	3′ End part of E3	1,912	5′-TACAAGGACCCCAA(G/C)AAGGCTA-3′	2520–2541
BE3-3A			5′-TACCACAGTTGGGAAGAGGG-3′	4431–4412
BE3-3AIN	3′ End part of E3	477	5′-CAGGCTATGCTACCA-3′	3508–3522
BE3-3SIN			5′-ATGAGTCGCTGTTCTGAGG-3′	3984–3966

↵ a Positions of the primers are indicated as those of Ad7 prototype (accession no. X76551 ) for hexon and Ad7h (accession no. Z48954 ) for fiber and the E3 region, respectively.

TABLE 3.

Genome types of Ad7 isolates and reference strains

Strain code (no. of strains tested)	Restriction pattern codes in panel of^a:			Genome type in this study
Strain code (no. of strains tested)	BgII, SacI	BamHI and others^a	BstEII	Genome type in this study
Ad7p (1)	1	1	1	Ad7p
Ad7a (1)	2	2	2	Ad7a
CH/90 (2)	2	3	2	Ad7d
NR/87 (2)	2	3	2	Ad7d
KY/87 (1)	2	3	2	Ad7d
AI/92 (19)	2	3	2	Ad7d
HR/95 (12)	2	3	3	Ad7d2
AK/95 (1)	2	3	3	Ad7d2
YM/95 (4)	2	3	3	Ad7d2
YK/95 (1)	2	3	3	Ad7d2
NR/95 (8)	2	3	3	Ad7d2

↵ a Restriction pattern codes correspond with the lane number in each panel in Fig. 1.
↵ b Other enzymes were BgIII, HindIII, PstI, PvuII, and SmaI.

TABLE 4.

Comparison of nucleotide sequences near 3′ end of E3 region among Ad7 strains

Strain code (no. of strains tested)	Accession no.	Nucleotide in PCR products at position^a:
Strain code (no. of strains tested)	Accession no.	186 (204)	247 (265)	276 (294)	342 (360)
Ad7p (1)	AB054824	A	G	T	G
Ad7a (1)	AB054825	C	G	C	G
CH/90 (2)	AB054818	C	G	C	A
NR/87 (1)	AB054819	C	G	C	A
KY/87 (1)	AB054820	C	G	C	A
AI/92 (2)	AB054821	C	G	C	A
HR/95 (1)	AB054822	C	A	C	A
YM/95 (1)	AB054823	C	A	C	A
Predicted amino acid substitution		Silent	Gly to Ser	Silent	Silent

↵ a Values in parentheses indicate the nucleotide positions in the 14.9-kDa protein-encoding open reading frame of the Ad7 prototype (Accession no. M23696 ).

TABLE 5.

Seroepidemiology of Ad7 and Ad3 infections in Hiroshima City in 1989