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Mycobacteriology and Aerobic Actinomycetes

Multicenter Evaluation of Fully Automated BACTEC Mycobacteria Growth Indicator Tube 960 System for Susceptibility Testing of Mycobacterium tuberculosis

Pascale Bemer, Frantiska Palicova, Sabine Rüsch-Gerdes, Henri B. Drugeon, Gaby E. Pfyffer
Pascale Bemer
1Department of Microbiology, Laënnec University Hospital, Nantes, France
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  • For correspondence: pascale.bemer@chu-nantes.fr
Frantiska Palicova
2Swiss National Center for Mycobacteria, Department of Medical Microbiology, Zurich, Switzerland
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Sabine Rüsch-Gerdes
3National Reference Center for Mycobacteria, Borstel, Germany
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Henri B. Drugeon
1Department of Microbiology, Laënnec University Hospital, Nantes, France
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Gaby E. Pfyffer
2Swiss National Center for Mycobacteria, Department of Medical Microbiology, Zurich, Switzerland
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DOI: 10.1128/JCM.40.1.150-154.2002
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  • TABLE 1.

    Reproducibility testing

    Drug (concn in μg/ml)No. of tests performedaNo. of results agreeing with reference method (BACTEC 460 TB)Agreement (%)
    INH (0.1)170170100
    INH (0.4)17016597.1
    RIF (1.0)17016999.4
    EMB (5.0)170170100
    EMB (7.5)16716699.4
    STR (1.0)17016999.4
    STR (4.0)170170100
     S   Total of tests1,1871,17999.3
    • ↵ a Center 1, 10 strains in duplicate at three different times (thus, six replicates per strain); center 2, 10 strains in triplicate at three different times (thus, nine replicates per strain); and center 3, 10 strains in triplicate (thus, two replicates per strain).

  • TABLE 2.

    Drug susceptibility results of clinical strains of M. tuberculosis as determined by BACTEC MGIT 960 and BACTEC 460 TB systema

    Drug (concn in μg/ml)No. of testsNo. of results that were:Agreement (%)
    S by both testsR by MGIT 960; S by 460 TBS by MGIT 960; R by 460 TBR by both tests
    INH (0.1)1108142596.4
    INH (0.4)29532189.7
    RIF (1.0)1109211799.1
    EMB (5.0)11093311396.4
    EMB (7.5)1773782.4
    STR (1.0)1107692591.8
    STR (4.0)3412101270.6
        Total of tests52036633112093.5
    • ↵ a S, susceptible; R, resistant.

  • TABLE 3.

    Resolution of discrepant results by proportion method on solid LJ mediuma

    Drug (concn in μg/ml)Initial results for:Resolved resultsb for:
    R by MGIT 960; S by 460 TBS by MGIT 960; R by 460 TBR by MGIT 960 and PM (true resistant)S by MGIT 960 and PM (true susceptible)R by MGIT 960; S by PM (false-resistant ME)
    INH (0.1)413
    INH (0.4)312
    RIF (1.0)110
    EMB (5.0)31013
    EMB (7.5)312
    STR (1.0)918
    STR (4.0)1064
        Total of tests33111122
    • ↵ a S, susceptible; R, resistant; PM, proportion method.

    • ↵ b Arbiter results based on the proportion method.

  • TABLE 4.

    Accuracy of BACTEC MGIT 960 compared with that of BACTEC 460 TB system after resolution of discrepanciesa

    Drug (concn in μg/ml)Sensitivity (%)Specificity (%)PPV (%)NPV (%)
    INH (0.1)10096.489.7100
    INH (0.4)10097.791.7100
    RIF (1.0)100100100100
    EMB (5.0)10096.981.3100
    EMB (7.5)10098.080100
    STR (1.0)10090.576.5100
    STR (4.0)10089.881.2100
    • ↵ a PPV, positive predictive value; NPV, negative predictive value.

  • TABLE 5.

    Time required for drug susceptibility testing of M. tuberculosis

    No. of strainsTime (days) required for:
    MGIT 960460 TB
    MedianRangeMedianRange
    85a6.34.6–11.77.04.0–10.0
    257.15.7–10.95.05.0–7.0
    1106.54.6–11.77.04.0–10.0
    • ↵ a Eighty-five strains were from center 1; 25 from center 2; and 110 from centers 1 and 2.

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Multicenter Evaluation of Fully Automated BACTEC Mycobacteria Growth Indicator Tube 960 System for Susceptibility Testing of Mycobacterium tuberculosis
Pascale Bemer, Frantiska Palicova, Sabine Rüsch-Gerdes, Henri B. Drugeon, Gaby E. Pfyffer
Journal of Clinical Microbiology Jan 2002, 40 (1) 150-154; DOI: 10.1128/JCM.40.1.150-154.2002

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Multicenter Evaluation of Fully Automated BACTEC Mycobacteria Growth Indicator Tube 960 System for Susceptibility Testing of Mycobacterium tuberculosis
Pascale Bemer, Frantiska Palicova, Sabine Rüsch-Gerdes, Henri B. Drugeon, Gaby E. Pfyffer
Journal of Clinical Microbiology Jan 2002, 40 (1) 150-154; DOI: 10.1128/JCM.40.1.150-154.2002
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KEYWORDS

Antitubercular Agents
Mycobacterium tuberculosis
Reagent Kits, Diagnostic

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